The determination of the effect of Curcumin on Saccharomyces cerevisiae totally protein expression changes and cell growth
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Keywords
Curcumin, H2O2, oxidative damage, protein, Saccharomyces cerevisiae, SDS-PAGE
Abstract
The use of turmeric for the treatment of various diseases stems mainly from its active biological functions, namely anti-inflammatory, antioxidant, anti-microbial, anti-alzheimer, anti-tumor, anti-diabetic and anti-rheumatism activities. Turmeric is a hypoglycemic, hepatoprotective, nephroprotective, cardioprotective and neuroprotective molecule, and it is reported to suppress thrombosis and protect against myocardial infarction. In this study, four groups were formed to investigate whether Curcumin has a protective role against the damage caused by hydrogen peroxide (H2O2) in Saccharomyces cerevisiae. Groups: (i) Control Group: Yeast cultivated group only; (ii) Curcumin Group: Curcumin group (% 8); (iii) H2O2 Group: Group given H2O2 (15 mM); (iv) Curcumin + H2O2 Group: Group given Curcumin (% 8) + H2O2 (15 mM). Saccharomyces cerevisiae cultures were developed at 30 °C for 1, 3, 5 and 24 hours. Cell growth, lipid peroxidation MDA (malondialdehyde) analysis and GSH (glutathione) levels were determined by spectrophotometer. Total protein changes were detected by SDS-PAGE electrophoresis and calculated by Bradford method. According to the results obtained; Cell growth (1, 3, 5 and 24 hours), total protein synthesis and GSH levels (24 hours) increased in Curcumin groups, while MDA level decreased (24 hours) when compared with H2O2 group. As a result, it was determined that Curcumin Saccharomyces cerevisiae culture has an effect that promotes cell growth and total protein synthesis as well as reducing oxidative damage.
References
2. Unal M. Effect of rations containing black pepper (Piper nigrum) and curcuma (Curcuma longa Linn) on the performance, egg yield, egg quality properties and blood parameters of hens. Cukurova Univ Institute Sci Techn 2019; Adana Turkey.
3. Akıncı N. Analysis of the effects of distinct chromatin modulators on NTH1 and TPS1 gene expression in yeast Saccharomyces cerevısiae. Çanakkale Onsekiz Mart Univ Manisa Turkey 2019.
4. Ekıncı S. The investigation of catalase localization of liquid tissue of curcumin-applied diabetic rats by immunohistochemical methods. Kafkas Univ Institute of Health Sci 2019; Kars Turkey.
5. Aslan A, Gok O, Erman O. The protective effect of kiwi fruit extract against to chromium effect on protein expression in Saccharomyces cerevisiae. Prog Nutr 2017; 19 (4): 472-476.
6. Jacewicz D, Siedlecka-Kroplewska K, Drzeżdżon J, Piotrowska A, Wyrzykowski D, Tesmar A, Zamojc K, et al. Method for detection of hydrogen peroxide in HT22 cells, Scien Rep 2017; 7: 45673.
7. Hewlings S, Kalman D. Curcumin: A review of its’ effects on human health. Foods 2017; 6 (10): 92-103.
8. Dai J, Gu L, Su Y, Wang Q, Zhao Y, Chen X, et al. Inhibition of Curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways. Int immunopharmacol 2018; 54: 177-187.
9. Aslan A, Beyaz S, Gok O. The protective effect of tomato extract against to chromium-induced damage in Saccharomyces cerevisiae. Erzincan Uni J Sci Tech 2019a; 12 (2): 1048-1055.
10. Aslan A, Gok O, Beyaz S. The protective effect of grape seed extract against to hydrogen peroxide-induced damage in Saccharomyces cerevisiae. Igdır Univ J Sci Tech 2019b; 9 (4): 2216-2224.
11. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 1970; 227: 680-685.
12. Cesurer G. The effect of magnesium on nitric oxide, malondialdehyde and glutathione in mice liver tissue fed on fat-diet. Kafkas Univ Institute of Health Sci 2015; Kars, Turkey.
13. Sosuncu E. Measurement of antioxidant enzymes and lipid peroxıdation levels in severe blunt head injuries. Yuzuncu Yıl Univ Institute of Health Sci 2015; Van Turkey.
14. Ghasemi F, Shafiee M, Banikazemi Z, Pourhanifeh MH, Khanbabaei H, Shamshirian A, et al. Curcumin inhibits NF-κB and Wnt/β-catenin pathways in cervical cancer cells. Pathology-Res Prac 2019; 215 (10): 152556.
15. Voulgaropoulou SD, Van Amelsvoort TAMJ, Prickaerts J, Vingerhoets C. The effect of Curcumin on cognition in alzheimer’s disease and healthy aging: A systematic review of pre-clinical and clinical studies. Brain Res 2019; 146476.
16. Mody D, AthamnehMohamed AIM, Seleem N. Curcumin: A natural derivative with antibacterial activity against Clostridium difficile. J Glob Antimicrob Res 2020; 21:154-161.
17. Aslan A. The effects of different essential fj and their combination on Saccharomyces cerevisiae cell growth. Prog Nutr 2015; 17 (1): 36-40.
18. Aslan A. Cell Culture developing and the imaging of total protein product changing with SDS-PAGE in Saccharomyces cerevisiae. Prog Nutr 2018; 20 (1): 128-132.
19. Samadian F, Dalili N, Gholi FPR, Fattah M, Malih N, Nafar M, et al. Evaluation of Curcumin's effect on inflammation in hemodialysis patients. Clin Nutr 2017; 22: 19-23.
20. Johnson JJ, Mukhtar H. Curcumin for chemoprevention of colon cancer. Cancer Lett 2007; 255 (2): 170-181.
21. Akbik D, Ghadiri M, Chrzanowski W, Rohanizadeh R. Curcumin as a wound healing agent. Life Sci 2014; 116 (1): 1-7.
22. Wongcharoen W, Phrommintikul A. The protective role of Curcumin in cardiovascular diseases. Int J Cardiol 2009; 133 (2): 145-151.
23. Martínez N, Herrera M, Frías L, Provencio M, Pérez-Carrión R, Díaz V, et al. A combination of hydroxytyrosol, omega-3 fatty acids and Curcumin improves pain and inflammation among early stage breast cancer patients receiving adjuvant hormonal therapy: Results of a pilot study. Clin Transl Oncol 2019; 21 (4): 489-498.
24. Eke D, Celik A, Yilmaz MB, Aras N, Sel SK, Alptekin D. Apoptotic gene expression profiles and DNA damage levels in rat liver treated with perfluorooctane sulfonate and protective role of Curcumin. Int J Bio Macromol 2017; 104: 515-520.
25. Cheng K, Yang A, Hu X, Zhu D, Liu K. Curcumin attenuates pulmonary inflammation in lipopolysaccharide induced acute lung injury in neonatal rat model by activating peroxisome proliferator-activated receptor γ (PPARγ) Pathway. Medical Science Monitor: Int Med J Exp Clin Res 2018; 24: 1178.
26. Salgıntas HH. The effects of Curcumin on antioxidant system at diabetic rats. Selcuk Univ Institute of Health Sci 2018; Konya Turkey.
27. Bırıncı H. The effect of Curcumin against cisplatin-caused kidney damage in rats. Gaziantep Univ Institute of Health Sci 2016; Gaziantep Turkey.
28. Gumuscu SA. Investigation of the possible protective effects of curcumin and epigallocatechin gallate against oxidative stress in rats. Mersin Univ Institute of Health Sci 2019; Mersin Turkey.
29. Saygılı Ü. The effect of Curcumin on some plasma cytokine levels at experimental diabetic rats. Selcuk Univ Institute of Health Sci 2018; Konya, Turkey.
30. Cotelı E, Karatas F. Determination of amounts of antioxidant vitamins and glutathione with total antioxidan capacity in plant Curcuma longa L. Erciyes Univ J Natur Appl Scie 2017; 33 (2): 91-101.
31. Plavcova Z, Salamunova P, Salon I, Stepanek F, Hanus J, Hosek J. Curcumin encapsulation in yeast glucan particles promotes its anti-inflammatory potential in vitro. Int J Pharm 2019; 568: 118532.
32. Kerdsomboon K, Tatip S, Kosasih S, Auesukaree C. Soluble Moringa oleifera leaf extract reduces intracellular cadmium accumulation and oxidative stress in Saccharomyces cerevisiae. J Bioscien Bioeng 2016; 121 (5): 543-549.
33. Jilani H, Cilla A, Barberá R, Hamdi M. Improved bioaccessibility and antioxidant capacity of olive leaf (Olea europaea L.) polyphenols through biosorption on Saccharomyces cerevisiae. Indust Crop Produc 2016; 84: 131-138.
34. Kiruthika B, Padma PR. Zea mays leaf extracts protect Saccharomyces cerevisiae cell against oxidative stress-induced cell death. J Acut Med 2013; 3 (3): 83-92.
35. Oprea E, Ruta LL, Nicolau I, Popa CV, Neagoe AD, Farcasanu IC. Vaccinium corymbosum L. (blueberry) extracts exhibit protective action against cadmium toxicity in Saccharomyces cerevisiae cells. Food Chem 2014; 152: 516-521.
36. Marques F, Azevedo F, Johansson B, Oliveira R. Stimulation of DNA repair in Saccharomyces cerevisiae by Ginkgo biloba leaf extract. Food ChemToxicol 2011; 49 (6): 1361-1366.
37. Selvam C, Prabu SL, Jordan BC, Purushothaman Y, Umamaheswari A, Zare MSH, et al. Molecular mechanisms of Curcumin and ıts analogs in colon cancer prevention and treatment. Life Sci 2019; 117032.
38. Huang Z, Yu Y, Fang Z, Deng Y, Shen Y, Shi P. OLE1 reduces cadmium-induced oxidative damage in Saccharomyces cerevisiae. FEMS Microbiol Lett 2018; 365 (18), fny193.
39. Albuz Ö. Investigation of cytotoxic effects of curcuma longa, zingiberaceae and Dianthus caryophyllus, which are commonly used as food supplements in daily life. Kocatepe Vet J 2019; 12 (3): 351-356.
40. Karaman BE, Köseler E. The relationship between turmeric and chronic diseases. Baskent Univ J Faculty Health Sci 2017; 2 (2): 96-112.
41. Beyaz S, Gok O, Aslan A. The indication of effect of ginger (Zingiber officinale) on Saccharomyces cerevisiae totally protein expression with SDS-PAGE technique. 1 st Int Malatya Congr App Sci 2019; 20-22 December Malatya Turkey.
42. Aslan A, Hussein YT, Gok O, Beyaz S, Erman O, Baspinar S. Ellagic acid ameliorates lung damage in rats via modulating antioxidant activities, inhibitory effects on inflammatory mediators and apoptosis-inducing activities. Env Sci Poll Res 2020a; 27(7): 7526-7537.
43. Aslan A, Beyaz S, Gok O, Erman O. The effect of ellagic acid on caspase-3/bcl-2/Nrf-2/NF-κB/TNF-α/COX-2 gene expression product apoptosis pathway: a new approach for muscle damage therapy. Mol Bio Rep 2020b; 47 (4): 2573–2582.
44. Gok O, Beyaz S, Aslan A. The investigation of effect of hawthorn fruit on Saccharomyces cerevisiae cell growth wıth molecular biological and biochemical process. 1 st Int Malatya Congr App Sci 2019; 20-22 December Malatya Turkey.
45. Aslan A, Gok O, Beyaz S, Arslan E, Erman O, Agca CA. The preventive effect of ellagic acid on brain damage in rats via regulating of Nrf-2, NF-κB and apoptotic pathway. J Food Biochem 2019c; 44 (6): e13217.
46. Mounce BC, Cesaro T, Carrau L, Vallet T, Vignuzzi M. Curcumin inhibits Zika and Chikungunya virus infection by inhibiting cell binding. Antivir Res 2017; 142: 148-157.
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Abdullah Aslan, Firat University, Faculty of Science, Department of Biology, Elazığ, Turkey
Firat University, Faculty of Science, Department of Biology, Elazığ, Turkey
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