Black cumin may be a potential drug for development of carbontetrachloride-induced lung damage in rats

Main Article Content

Abdullah Aslan
Didem Boydak
Muhammed Ismail Can
Tuncay Kuloglu
Serpil Baspinar

Keywords

Apoptosis, DNA damage, lung, new drug, TUNEL

Abstract

The study examines whether Black cumin (Nigella sativa L.) plays a protective role against the damage in the lung by administering carbontetrachloride (CCl4) to rats. 28 male Wistar albino (n=28, 8 weeks old) rats were used in the study. The rats divided into 4 groups according to their live weights. The groups were: (i) Negative Control (NC): Normal water consuming group to which no CCl4 and Black cumin (BC) is administered; (ii) Positive Control (PC): Normal water consuming group to which no CCl4 is administered but BC is administered; (iii) CCl4 Group: Normal water consuming and group to which CCl4 is administered (1.5 ml/kg live weight, ip); (iv) CCl4 + BC group: CCl4 and BC administered group (1.5 ml/kg live weight, ip). Tissue apoptotic index was examined via TUNEL method. MDA (malondialdehyde) determination in lung tissue was made using spectrophotometer. As a results, MDA amount decreased in the CCl4 + BC group (6,33 ± 1,54 nmol/g) in comparison to CCl4 group (8,66 ± 1,58 nmol/g) whereas it was observed in the CCl4+BC group (15,35 ± 0,21%) that the apoptotic index (TUNEL results) decreases in comparison with the CCl4 group (27,48 ± 0,28%) thus approaching normal values. DNA damage ratio decreased in the CCl4 + BC group in comparison to CCl4 group. These results show that BC plant protects the lung against oxidative damage.

Downloads

Download data is not yet available.
Abstract 241 | PDF Downloads 151