Main Article Content
Cell growth, Electrophoresis, Protein synthesis, Royal jelly, Saccharomyces cerevisiae
Study Objective: Royal jelly is a bee product that has a nutritious value and has been used in the treatment of many diseases since ancient times. Royal jelly has the ability to scavenge free radicals thanks to its antifungal, antiviral, antimicrobial, anti-inflammatory and antioxidant effects.
Methods: In this study, four groups were created to investigate whether Royal jelly has a protective role against copper chloride (CuCl2) damage in Saccharomyces cerevisiae. Study groups: (1) Control Group: Group in which only yeast was cultivated; (2) CuCl2 Group: the group given CuCl2 (30 mM); (3) Royal Jelly Group: The group given Royal jelly (10%); (4) Royal Jelly + CuCl2 Group: The group given Royal jelly (10%) + CuCl2 (30 mM). Saccharomyces cerevisiae cultures were grown at 30 °C for 1, 3, 5 and 24 hours. Cell growth, GSH (glutathione) levels, CAT (catalase) activities and lipid peroxidation MDA (malondialdehyde) analyzes were determined by spectrophotometer. Total protein concentrations were determined by SDS-PAGE electrophoresis and Lowry protein method.
Results: when compared with the CuCl2 group, cell growth (1, 3, 5 and 24 hours), total protein synthesis and GSH level (24 hours) increased in Royal jelly groups, while MDA level (24 hours) decreased.
Conclusions: Thanks to the antioxidant properties of Royal jelly, it has been determined that it increases cell growth and total protein synthesis by reducing oxidative stress in Saccharomyces cerevisiae culture. It has been concluded that these natural products also have strong therapeutic effects in the treatment of many diseases.
2. Seven I, Simsek UG., Gokce Z, Tatlı Seven P, Arslan A, Yılmaz O. The effects of royal jelly on performance and fatty acid profiles of different tissues in quail (Coturnix coturnix japonica) reared under high stocking density. Turk J Vet Ani Sci. 2014; 38: 271-277.
3. Aslan A, Gok, O. Erman, O. The protective effect of kiwi fruit extract against to chromium effect on protein expression in Saccharomyces cerevisiae. Prog Nutr. 2017; 19 (4): 472-478.
4. Keskın M, Ozkok A, Karahalil F, Kolaylı S. What should be the amount of 10- hydroxi-2-decanoic acid (10-hda) in royal jelly?. Med Agr Sci. 2020; 33 (3): 347-350.
5. Mutlu S. Investigation of changes in the expression levels of flocculation, adhesion and oxidatıve stress-related genes in stress-resistant Saccharomyces cerevisiae mutants. Istanbul Technic Unıv Istanbul Turkey 2010.
6. Bayram D, Oncu M, Ozcelik N, Yılmaz H, Uz E. Gokcımen A, Ozgoçmen M. The effects of thiopenthone sodium and propofol on rat liver. Med J Suleyman Demirel Univ. 2014; 5 (2): 36-44.
7. Aslan A, Beyaz S, Gok, O. The protective effect of tomato extract against to chromium-ınduced damage in Saccharomyces cerevisiae. Erz Univ J Sci. 2019a; 12 (2): 1048-1055.
8. Ozkaya A. Effect of bıochanın a on the some bıochemıcal parameters in serum of rats ınduced oxıdatıve stres. Eastern Anatol Reg Stud, 2008; 1-5.
9. Aslan A, Gok O, Beyaz S. The protective effect of grape seed extract against to hydrogen peroxide-induced damage in Saccharomyces cerevisiae. Igdır Univ J Sci Techn. 2019b; 9 (4): 2216-2224.
10. Babele PK, Thakre PK, Kumawat R, Tomar RS. Zinc oxide nanoparticles induce toxicity by affecting cell wall integrity pathway, mitochondrial function and lipid homeostasis in Saccharomyces cerevisiae. Chemosphere. 2018; 213: 65-75.
11. Beyaz S, Dalkılıç LK, Gok O, Aslan A. The effect of black mulberry (Morus nigra L.) and Cranberry (Cornus mas L.) on some molecular biological and biochemical parameters against oxidative damage caused by hydrogen peroxide in Saccharomyces cerevisiae. Bitlis Eren Univ J Sci. 2020; 9 (3): 1134-1144.
12. Aslan A. The effects of different essential fruit juice and their combination on Saccharomyces cerevisiae cell growth. Prog Nutr. 2015; 17 (1): 36-40.
13. Albuz O. Investigation of cytotoxic effects of Curcuma longa, Zingiberaceae and Dianthus caryophyllus, which are commonly used as food supplements in daily life. Kocatepe Vet J. 2019; 12 (3): 351-356.
14. Huang Z, Yu Y, Fang Z, Deng Y, Shen Y, Shi P. OLE1 Reduces cadmium-ınduced oxidative damage in Saccharomyces cerevisiae. FEMS Microbiol Lett. 2018; 365 (18): fny193.
15. Vázquez J, González B, Sempere V, Ma, A, Torija MJ, Beltran G. Melatonin reduces oxidative stress damage induced by hydrogen peroxide in Saccharomyces cerevisiae. Front in Microbiol. 2017; 8: 1066.
16. Aslan A. Cell culture developing and the ımaging of total protein product changing with SDS-PAGE in Saccharomyces cerevisiae. Prog Nutr. 2018; 20 (1): 128-132.
17. Almeer RS, Kassab RB, AlBasher GI, Alarifi S, Alkahtani S, Ali D, Moneim AEA. Royal jelly mitigates cadmium-ınduced neuronal damage in mouse cortex. Mol Bio Rep. 2019a; 46: 119-131.
18. Mohamed AAR, Galal AA, Elewa YH. Comparative protective effects of royal jelly and cod liver oil against neurotoxic impact of tartrazine on male rat pups brain. Acta Histochemica. 2015; 117 (7): 649-658.
19. Almeer RS, AlBasher GI, Alarifi S, Alkahtani S, Ali D, Moneim AEA. Royal jelly attenuates cadmium-ınduced nephrotoxicity in male mice. Sci Rep. 2019b; 9: 1-12.
20. Salahshoor MR, Jalili C, Roshankhah S. Can royal jelly protect against renal ıschemia/reperfusion ınjury in rats?. Chinese J Physiol. 2019; 62 (3): 131.
21. Volarevic V, Djokovic B, Jankovic MG, Harrell CR, Fellabaum C, Djonov V, Arsenijevic N. Molecular mechanisms of cisplatin-ınduced nephrotoxicity: A balance on the knife edge between renoprotection and tumor toxicity. J Biomedi Sci. 2019; 26 (1): 1-14.
22. Ohashi E, Kohno K, Arai N, Harashima A, Ariyasu T, Ushio S. Adenosine N1-oxide exerts anti-ınflammatory effects through the PI3K/Akt/GSK-3β signaling pathway and promotes osteogenic and adipocyte differentiation. Bio Pharm Bulletin. 2019; 42 (6): 968-976.
23. Jamnik P, Goranovic D, Raspor P. Antioxidative action of royal jelly in the yeast cell. Exp Gerontol. 2007; 42(7): 594-600.
24. Soylu P. Functional food production from the mixture of honey, propolis, bee milk, nailple (Achillea millefolium) and Echinacea (Echinacea paradoxa) herbs. Gumushane Unıv Gumushane Turkey 2019.
25. Yavas S. Effect of royal jelly on apoptosis, proliferation and immunoreactivite of INSL3 in rat diabetic testes tissue. Trakya Unıv Trakya Turkey 2017.
26. Zorer B. Investigation on the antiapoptotic, antigenotoxic and antioxidant effects of Royal jelly by molecular, biochemical and stereological methods in ultraviolet b-ınduced skin damage model of albino rats. Yuzuncu Yıl Unıv Van Turkey 2016.
27. Kaynar L. Effects of royal jelly on methotrexate-ınduced ıntestinal mucosa damage and Systemic oxidative stress in rats. Ercıyes Univ Kayseri Turkey 2010.
28. Saral O. Bioactive properties of apitherapy bee products (honey, pollen, propolis and royal jelly) and their roles in prevention liver damage. Karadeniz Teknik Unıv Trabzon Turkey 2013.
29. Atabay NO. Effect of royal jelly on fertility in >akkaraman ewes. Erciyes Unıv Kayseri Turkey 2012.
30. Aslan A, Beyaz S, Gok O, Can MI, Erman F, Erman O. The impact of ellagic acid on some apoptotic gene expressions: a new perspective for the regulation of pancreatic Nrf-2/NF-κB and Akt/VEGF signaling in CCl4-induced pancreas damage in rats. Immunopharmacology and Immunotoxicol. 2021a; 43 (2): 145-152.
31. Aslan A, Gok O, Beyaz S, Agca CA, Erman O, Zerek A. Ellagic acid prevents kidney injury and oxidative damage via regulation of Nrf-2/NF-κB signaling in carbon tetrachloride induced rats. Mol Bio Rep. 2020d; 47 (10): 7959–7970.
32. Aslan A, Can MI. The effect of orange juice against to H2O2 stress in Saccharomyces cerevisiae. Prog Nutr. 2015; 17 (3): 250-254.
33. Aslan A, Can MI. The inhbition of chromium effect in Saccharomyces cerevisiae thrive from grapefruit. Prog Nutr. 2015; 17 (4): 339-342.
34. Aslan A, Can MI, Kuloglu T, Baspinar S. Milk thistle may induce apoptosis in development of carbontetrachloride-induced liver DNA damage in rats. Prog Nutr. 2016a; 18(2): 146-151.
35. Aslan A, Can MI. Protein expression product alterations in Saccharomyces cerevisiae. Progr Nutr. 2017; 19(1): 81-85.
36. Aslan A, Boydak D, Can MI, Kuloglu T, Baspinar S. Black cumin may be a potential drug for development of carbontetrachloride-induced lung damage in rats. Prog Nutr. 2016b; 18(1): 56-62.