Gundelia tournefortii L. (Kenger): Determination of in vitro Antidiabetic Activities: An edible wild natural food: Gundelia tournefortii L. (Kenger)

Merve Keskin; Gulsen Kaya; Saban  Keskin

doi:10.23751/pn.v23i4.11079

Gundelia tournefortii L. (Kenger): Determination of in vitro Antidiabetic Activities

An edible wild natural food: Gundelia tournefortii L. (Kenger)

Authors

Merve Keskin Vocational School of Health Services, Bilecik Şeyh Edebali University, Bilecik, Turkey
Gulsen Kaya Scientific and Technological Research Center, Inonu University, Malatya, Turkey
Saban Keskin Vocational School of Health Services, Bilecik Seyh Edebali University, Bilecik, Turkey

Keywords:

DPPH, FRAP, Diabetes mellitus, Gundelia tournefortii L, α-amylase, α-glucosidase

Abstract

Diabetes mellitus is a chronic disease that negatively effects daily life. Synthetic drugs, which are inhibitors of α-glucosidase enzymes, are used in its treatment. These drugs may have reaction on organs such as the liver. Therefore, it is important to include plants that inhibit these enzymes in the diet in the treatment of Diabetes mellitus. Gundelia tournefortii L. (Kenger) is an edible wild natural food that is widely used as traditional food. In this study, the biochemical characterization of the Gundelia tournefortii L. (Kenger) extracts were performed and the inhibition effects on α-amylase and α-glycosidase enzymes were investigated. Total phenolic content, antioxidant activity and chemical composition of Gundelia tourneforti L. extracts were determined. The chemical composition of extracts were determined GC/MS. IC₅₀ values of α-amylase and α-glucosidase enzymes of the Gundelia tournefortii L. ethanolic and water extracts were determined as 4.18 ± 0.08 mg/mL and 5.17 ± 0.9 mg/mL, respectively. The results showed that Gundelia tournefortii L. could be used as a supplementary food in the treatment of Type 2 diabetes.

References

Kahn CR, Weir GC, King GL, Jacobson AM, Moses AC, Smith RJ: Joslin’s Diabetes Mellitus. 14.th Edition. Lippincott Willams-Wilkins, 2005.

Blair M. Diabetes mellitus review. Urologic Nursing. 2016; 36(1): 27-36.

Ríos JL, Francini F, Schinella GR. Natural products for the treatment of type 2 diabetes mellitus. Planta medica. 2015; 81: 975–994. DOI: 10.1055/s-0035-1546131

Dağlar N, Dağdeviren HN. Geleneksel ve Tamamlayıcı Tıp Uygulamalarında Fitoterapinin Yeri. Eurasian journal of family medicine. 2018; 7(3):73-7.

Can Z, Baltaş N, Keskin S, Yıldız O, Kolaylı S. Properties of antioxidant and anti-inflammatory activity and phenolic profiles of Şevketi Bostan (Cnicusbenedictus L.) cultivated in Aegean Region from Turkey. Turkish Journal of Agriculture-Food Science and Technology. 2017; 5(4): 308-314. DOI: 10.24925/turjaf.v5i4.308-314.1054

Kaya G, Keskin M. Biochemical properties and urease, α-amylase inhibitory effects of Ocimum basilicum L. (Reyhan). Journal of Medicinal Herbs and Ethnomedicine. 2020; 06: 52-55. DOI: 10.25081/jmhe.2020.v6.6248

Konak M, Ateş M, Şahan Y. Yenilebilir yabani bitki Gundelia tournefortii’nin antioksidan özelliklerinin belirlenmesi. Uludağ Üniversitesi Ziraat Fakültesi Dergisi. 2017; 31(2): 101-108.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amer J Enol Viticult. 1965;16:144-158.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology. 1999; 299:152-178. DOI: 10.1016/S0076-6879(99)99017-1

Fukumoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. Journal Agriculture Food Chemistry. 2000;48:3597-3604. DOI: 10.1021/jf000220w

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical biochemistry. 1996; 239(1): 70-76.

DOI: 10.1006/abio.1996.0292.

Cuendet M, Hostettmann K, Potterat O. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta. 1997; 80:1144-1152. DOI: 10.1002/hlca.19970800411

Keskin Ş, Ertunga NS. Purification, immobilization and characterization of thermostable α-amylase from a thermophilic bacterium Geobacillus sp. TF14. Turk J Biochem. 2017; 42(6): 637–646. DOI: 10.1515/tjb-2016-0123

Gholamhoseinian A, Fallah H, Sharifi-far F, Mirtajaddini M. The inhibitory effect of some Iranian plants extracts on the alpha glucosidase. Iranian Journal of Basic Medical Sciences. 2008;11(1): 1-9. DOI: 10.22038/ijbms.2008.5190

Aksoy H, Bingöl Özakpinar Ö. Yara iyileşmesi ve oksidatif stress. Marmara Pharmaceutical Journal. 2014; 3: 153-158. DOI: 10.12991/mpj.2014187243

Halifeoğlu İ, Karataş F, Çolak R, Canatan H, Telo S. Tip 2 Diyabetik Hastalarda Tedavi Öncesi ve Tedavi Sonrası Oksidan ve Antioksidan Durum. Fırat Tıp Dergisi. 2005; 10(3): 117-122.

Demir E, Yılmaz Ö. The effect of pine oil on the some alterations in liver tissue of experimental diabetes. Turkish Bulletin of Hygiene and Experimental Biology. 2014; 71(3): 113-1. DOI: 10.5505/TurkHijyen.2014.45822

Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos University Medical Journal. 2012; 12(1): 5. DOI: 10.12816%2F0003082

Öntürk H, Özbek H. Deneysel diyabet oluşturulması ve kan şeker seviyesinin ölçülmesi. Genel Tıp Dergisi. 2007; 17(4): 231-6.

Dalar A, Zengin G, Mukemre M, Bengu AS, İşler S. Gundeliarosea seed: Evaluation of biopharmaceutical potential and bioactive composition. South African Journal of Botany. 2019;125:505-510.

Coruh N, Celep AS, Özgökçe F, İşcan M. Antioxidant capacities of Gundeliatournefortii L. extracts and inhibition on glutathione-S-transferase activity. Food Chemistry. 2007; 100(3):1249-1253. DOI: 10.1016/j.foodchem .2005. 12.008

Farhang HR, Vahabi MR, Allafchian AR. Chemical compositions of the essential oil of Gundeliatournefortii L.(Asteraceae) from Central Zagros, Iran. Journal of Herbal Drugs (An International Journal on Medicinal Herbs). 2016;6(4):227-233.

Kadan S, Sasson Y, Saad B, Zaid H. Gundelia tournefortii Antidiabetic Efficacy: Chemical Composition and GLUT4 Translocation. Evidence-Based Complementary and Alternative Medicine. 2018; 8p.