Gundelia tournefortii L. (Kenger): Determination of in vitro Antidiabetic Activities An edible wild natural food: Gundelia tournefortii L. (Kenger)
Main Article Content
Keywords
DPPH, FRAP, Diabetes mellitus, Gundelia tournefortii L, α-amylase, α-glucosidase
Abstract
Diabetes mellitus is a chronic disease that negatively effects daily life. Synthetic drugs, which are inhibitors of α-glucosidase enzymes, are used in its treatment. These drugs may have reaction on organs such as the liver. Therefore, it is important to include plants that inhibit these enzymes in the diet in the treatment of Diabetes mellitus. Gundelia tournefortii L. (Kenger) is an edible wild natural food that is widely used as traditional food. In this study, the biochemical characterization of the Gundelia tournefortii L. (Kenger) extracts were performed and the inhibition effects on α-amylase and α-glycosidase enzymes were investigated. Total phenolic content, antioxidant activity and chemical composition of Gundelia tourneforti L. extracts were determined. The chemical composition of extracts were determined GC/MS. IC50 values of α-amylase and α-glucosidase enzymes of the Gundelia tournefortii L. ethanolic and water extracts were determined as 4.18 ± 0.08 mg/mL and 5.17 ± 0.9 mg/mL, respectively. The results showed that Gundelia tournefortii L. could be used as a supplementary food in the treatment of Type 2 diabetes.
References
2. Blair M. Diabetes mellitus review. Urologic Nursing. 2016; 36(1): 27-36.
3. Ríos JL, Francini F, Schinella GR. Natural products for the treatment of type 2 diabetes mellitus. Planta medica. 2015; 81: 975–994. DOI: 10.1055/s-0035-1546131
4. Dağlar N, Dağdeviren HN. Geleneksel ve Tamamlayıcı Tıp Uygulamalarında Fitoterapinin Yeri. Eurasian journal of family medicine. 2018; 7(3):73-7.
5. Can Z, Baltaş N, Keskin S, Yıldız O, Kolaylı S. Properties of antioxidant and anti-inflammatory activity and phenolic profiles of Şevketi Bostan (Cnicusbenedictus L.) cultivated in Aegean Region from Turkey. Turkish Journal of Agriculture-Food Science and Technology. 2017; 5(4): 308-314. DOI: 10.24925/turjaf.v5i4.308-314.1054
6. Kaya G, Keskin M. Biochemical properties and urease, α-amylase inhibitory effects of Ocimum basilicum L. (Reyhan). Journal of Medicinal Herbs and Ethnomedicine. 2020; 06: 52-55. DOI: 10.25081/jmhe.2020.v6.6248
7. Konak M, Ateş M, Şahan Y. Yenilebilir yabani bitki Gundelia tournefortii’nin antioksidan özelliklerinin belirlenmesi. Uludağ Üniversitesi Ziraat Fakültesi Dergisi. 2017; 31(2): 101-108.
8. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amer J Enol Viticult. 1965;16:144-158.
9. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology. 1999; 299:152-178. DOI: 10.1016/S0076-6879(99)99017-1
10. Fukumoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. Journal Agriculture Food Chemistry. 2000;48:3597-3604. DOI: 10.1021/jf000220w
11. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical biochemistry. 1996; 239(1): 70-76.
DOI: 10.1006/abio.1996.0292.
12. Cuendet M, Hostettmann K, Potterat O. Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta. 1997; 80:1144-1152. DOI: 10.1002/hlca.19970800411
13. Keskin Ş, Ertunga NS. Purification, immobilization and characterization of thermostable α-amylase from a thermophilic bacterium Geobacillus sp. TF14. Turk J Biochem. 2017; 42(6): 637–646. DOI: 10.1515/tjb-2016-0123
14. Gholamhoseinian A, Fallah H, Sharifi-far F, Mirtajaddini M. The inhibitory effect of some Iranian plants extracts on the alpha glucosidase. Iranian Journal of Basic Medical Sciences. 2008;11(1): 1-9. DOI: 10.22038/ijbms.2008.5190
15. Aksoy H, Bingöl Özakpinar Ö. Yara iyileşmesi ve oksidatif stress. Marmara Pharmaceutical Journal. 2014; 3: 153-158. DOI: 10.12991/mpj.2014187243
16. Halifeoğlu İ, Karataş F, Çolak R, Canatan H, Telo S. Tip 2 Diyabetik Hastalarda Tedavi Öncesi ve Tedavi Sonrası Oksidan ve Antioksidan Durum. Fırat Tıp Dergisi. 2005; 10(3): 117-122.
17. Demir E, Yılmaz Ö. The effect of pine oil on the some alterations in liver tissue of experimental diabetes. Turkish Bulletin of Hygiene and Experimental Biology. 2014; 71(3): 113-1. DOI: 10.5505/TurkHijyen.2014.45822
18. Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos University Medical Journal. 2012; 12(1): 5. DOI: 10.12816%2F0003082
19. Öntürk H, Özbek H. Deneysel diyabet oluşturulması ve kan şeker seviyesinin ölçülmesi. Genel Tıp Dergisi. 2007; 17(4): 231-6.
20. Dalar A, Zengin G, Mukemre M, Bengu AS, İşler S. Gundeliarosea seed: Evaluation of biopharmaceutical potential and bioactive composition. South African Journal of Botany. 2019;125:505-510.
21. Coruh N, Celep AS, Özgökçe F, İşcan M. Antioxidant capacities of Gundeliatournefortii L. extracts and inhibition on glutathione-S-transferase activity. Food Chemistry. 2007; 100(3):1249-1253. DOI: 10.1016/j.foodchem .2005. 12.008
22. Farhang HR, Vahabi MR, Allafchian AR. Chemical compositions of the essential oil of Gundeliatournefortii L.(Asteraceae) from Central Zagros, Iran. Journal of Herbal Drugs (An International Journal on Medicinal Herbs). 2016;6(4):227-233.
23. Kadan S, Sasson Y, Saad B, Zaid H. Gundelia tournefortii Antidiabetic Efficacy: Chemical Composition and GLUT4 Translocation. Evidence-Based Complementary and Alternative Medicine. 2018; 8p.
Article Sidebar
Article Details
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Transfer of Copyright and Permission to Reproduce Parts of Published Papers.
Authors retain the copyright for their published work. No formal permission will be required to reproduce parts (tables or illustrations) of published papers, provided the source is quoted appropriately and reproduction has no commercial intent. Reproductions with commercial intent will require written permission and payment of royalties.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
