The Determination of heavy metals in wine products in Montenegro in order to protect consumer health Determination of heavy metals in wine products in Montenegro in order to protect consumer health

Main Article Content

Tanja Vojinovic
Marko Jaukovic
Zorica Potpara
Sehija Dizdarevic
Refik Zejnilovic

Keywords

Potentiometric stripping analysis; Heavy metals; Red and Rosé wines; Human health protection

Abstract

Summary. Background/Aim: Wine may contain essential macronutrients and mircoelements, but it can also be a source of heavy metals. Heavy metals significantly affect the quality and shelf-life of wine, even when present in micro- quantities. The aim of this study is to investigate the possibility of determining the content of heavy metals, zinc, cadmium, lead, copper, in the red and rosé wines samples, produced by the Montenegro, using potentiometric stripping analysis. Methods: Testing 12 samples of the red and rosé wines, produced by Montenegro, in five replications, were performed using the potentiometric stripping analysis (PSA) technique, whereas comparative analyses were performed using atomic absorption spectrometry (AAS) by flame and graphite technique. Results: The results of testing a total of 12 red and rosé wine samples using potentiometric striping analysis (PSA) and flame atomic absorption analysis (AAS) and graphite technique, indicate that the heavy metals content of Zn, Cd, Pb and Cu was in the range (values are expressed in ppb) of: 277.14 - 305.5; 8.98 - 13.83; 45.87 - 59.94 for the red wine; and 14.21 - 19.02: 321.88 - 414.58; 3.05 - 4.41; 36.88 - 44.56; 19.48 - 22.17 for the rosé wine. Conclusion: The results obtained in this study, as well as the complexity and the duration of the analysis, lead to the conclusion that it is justified to employ potentiometric stripping analysis in determining the heavy metal content in wine.


 


          Key words: Potentiometric stripping analysis; Heavy metals; Red and Rosé wines; Human health protection

Downloads

Download data is not yet available.
Abstract 18 | PDF Downloads 3

References

[1] Jackson RS. 2008. Wine Science: Principles and Applications, 3rd ed. Elsevier, London.
[2] Snopek L, Mlcek J, Sochorova L et al. Contribution of Red Wine Consumption to Human Health Protection. Molecules 2018; 23 (7).
[3] Sun AY, Wang Q, Simonyi A, Sun GY. Botanical phenolics and brain health. Neuromolecular. Med. 2008; 10:259–274.
[4] Semla M, Schwarcz P, Mezey J et al. Biogenic and Risk Elements in Wines from the Slovak Market with the Estimation of Consumer Exposure. Biol Trace. Elem. Res. 2018; 184:33-41.
[5] Wang S, Shi X. Molecular mechanisms of metal toxicity and carcinogenesis. Mol. Cell. Biochem. 2001; 222:3-9.
[6] Lara R, Cerutti S, Salonia JA, Olsina RA, Martinez LD. Trace element determination of Argentine wines using ETAAS and USN-ICP-OES. Food Chem.Toxicol. 2005; 43:293-7.
[7] Galani-Nikolakaki S, Kalithrakas-Kontos N, Katsanos AA. Trace element analysis of Cretan wines and wine products. Sci. Total. Environ. 2002. 285:155-63.
[8] Towle KM, Garnick LC, Monnot AD. A human health risk assessment of lead (Pb) ingestion among adult wine consumers. Int. J. Food Contam. 2017; 4(1), 7.
[9] Prozialeck WC, Edwards JR. Mechanisms of cadmium-induced proximal tubule injury: new insights with implications for biomonitoring and therapeutic interventions. J. Pharmacol. Exp. Ther. 2012; 343:2–12.
[10] Robards K, Worsfold P. Cadmium: toxicology and analysis. A review. Analyst. 1991; 116:549-68.
[11] Sun X, Ma T, Yu J, Huang W, Fang Y, Zhan J. Investigation of the copper contents in vineyard soil, grape must and wine and the relationship among them in the Huaizhuo Basin Region, China: a preliminary study. Food Chem. 2018; 241:40-50.
[12] Zerbinati O, Balduzzi F, Dell'Oro V. Determination of Lithium in wines by ion chromatography. J. Chromatogr. A. 2001; 881:645-50.
[13] Pyrzinska K. Analitical methods for the determination of trace metals in wine. Cri.t Rev. Anal. Chem. 2004; 34:69-83.
[14] Castiñeira Gómez Mdel M, Brandt R, Jakubowski N, Andersson JT. Changes of the metal composition in German white wines through the winemaking process. A study of 63 elements by inductively coupled plasma-mass spectrometry. J. Agric. Food Chem.2004; 52:2953-61.
[15] Jagner D, Graneli A. Potentiometric stripping analysis. Anal.Chim. Acta. 1976; 83:19-26.
[16] Suturovic Z. 2003. Elektrochem. Stripping Analysis, p.120. Faculty of Technology, Novi Sad.
[17] Suturovic Z. 1992. Povećanje osetljivosti potenciometrijske striping analize [Increasing the sensitivity of potentiometric striping analysis; in Serbian ] [PhD thesis]. Faculty of Technology, University of Novi Sad, Novi Sad.
[18] ISO 5515:2003: Voće, povrće i proizvodi od voća i povrća – Razgradnja organske materije prije analize –Metoda vlažnog postupka [ISO 5515:2003: Fruits, vegetables and fruit and vegetable products - Decomposition of organic materials before analysis - Wet method].
[19] Savic J, Savic M. 1990. Osnovi analitičke metode, klasične metode. 3 izd. Svjetlost Zavod za udžbenike i nastavna sredstva, Sarajevo. [ Savic J, Savic M.1990. Fundamentals of analytical methods, classical methods. 3rd ed. Light Institute of Textbooks and Teaching Aids, Sarajevo].
[20] Pravilnik o dozvoljenim količinama teških metala, mikotoksina i drugih supstanci u hrani (Sl.list CG br.81/2009 i 55/2015) [Ordinance on the permissible levels of heavy metals, mycotoxins, and other substances in food. Off. journal MNE No 81/2009 and 55/2015]. ained via potentiometric stripping analysis (PSA) and atomic absorption spectrophotometry (AAS); the means, standard deviation and maximum permitted levels
[21] Arcos MT, Ancin MC, Echeverria JC, Gonzalez A, Garrido JJ. Study of lability of heavy metals in wines with different degrees of aging through differential pulse anodic stripping voltammetry. J. Agric. Food Chem, 1993; 41(12), 2333-2339.
[22] Ibanez JG, Carreon- Alvarez A, Barcena- Soto M, Casillas N. Metals in alcoholic beverages: A review of sources, effects, concentrations, removal, speciation and analysis. J. Food Compos. Anal., 2008; 21: 672–683.
[23] Kaufman A. 1998. Lead in wine, Food additives and contaminants, 15:437-445.
[24] Teissedre PL, Lobinski R, Cabanis MT, Szpunar-Lobinska J, Cabanis JC, Adams FC. On the origin of organolead compounds in wine, Sci. Total Environ. 1994; 153 (3): 247-252.
[25] Bora FD, Bunea CI, Rusu T, Pop N. Vertical distribution and analysis of micro, macroelements and heavy metals in the system soil- grapevine- wine in vineyard from North- West Romania. Chem Cent J. 2015; 9:1.
[26] Elçi L, Arslan Z, Tyson JF. Determination of lead in wine and rum samples by flow injection-hydride generation-atomic absorption spectrometry. J Hazard Mater. 2009; 162:880–5.
[27] Illuminati S, Annibaldi A, Truzzi C, Scarponi G. Recent temporal variations of trace metal content in an Italian white wine. Food Chem. 2014; 159:493–7.
[28] Karadjova IB, Lampugnani L, D’Ulivo A, Onor M, Tsalev D. Determination of lead in wine by hydride generation atomic fluorescence spectrometry in the presence of hexacyanoferrate (III). Anal Bioanal Chem. 2007; 388:801–7.
[29] La Pera L, Dugo G, Rando R, Di Bella G, Maisano R, Salvo F. Statistical study of the influence of fungicide treatments (mancozeb, zoxamide and copper oxychloride) on heavy metal concentrations in Sicilian red wine. Food Addit Contam. 2008; 25:302–13.
[30] Catarino S, Curvelo-Garcia A, De Sousa RB. Measurements of contaminant elements of wines by inductively coupled plasma-mass spectrometry: a comparison of two calibration approaches. Talanta. 2006; 70:1073–80.
[31] Catarino S, Madeira M, Monteiro F, Rocha F, Curvelo-Garcia A, De Sousa RB. Effect of bentonite characteristics on the elemental composition of wine. J Agric Food Chem. 2007; 56:158–65.
[32] Da Costa AS, Delgadillo I, Rudnitskaya A. Detection of copper, lead, cadmium and iron in wine using electronic tongue sensor system. Talanta. 2014; 129:63–71.
[33] Santos S, Lapa N, Alves A, Morais J, Mendes B. Analytical methods and validation for determining trace elements in red wines. J Environ Sci Health B. 2013; 48:364–75
[34] Yamasaki A, Oliveira JA, Duarte AC, Gomes MTS. An insight into the adsorption and electrochemical processes occurring during the analysis of copper and lead in wines, using an electrochemical quartz crystal nanobalance. Talanta. 2012; 98:14–8.