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S.cerevisiae, Pistacia vera, CCl4, Oxidative damage, Antioxidant systems, Flavonoid
Summary. In this study, phytochemical ingredients of Pistacia vera cultivated in Kilis province, and it was investigated to demonstrate anti-oxidant effects and on some biochemical parameters and of this content on CCl4-induced cell damage in Saccharomyces cerevisiae.
Pistacia vera fruits were extracted and then subjected to flavonoid analysis by HPLC device. S. cerevisiae (bread yeast) was used as a cell culture model. For the development and proliferation of S. cerevisiae FMC16 in the study, YEDP (1 g yeast extract for 100 ml, 2 g bactopeptone, 2 g glucose) medium was used.
In this study, six groups were composed. i: Control group, ii: CCl4 (100 µl) group, iii: Pistacia vera 200 µl (PV2) group, iv: Pistacia vera 400 µl (PV4) group, v: PV2+CCl4 group and vi: PV4+CCl4 group. After sterilization, PV and CCl4 were inserted to S. cerevisiae cultures and the cultures were developed at 60°C for 72 hour (overnight). Antioxidant enzymes activities such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSHpx), glutathione S-transferase (GST) and glutathione reductase (GR) were determined in S. cerevisiae cell by spectrophotometer. Glutathione (GSH), oxidized glutathione (GSSG) and malondialdehyde (MDA) were investigated in S. cerevisiae cell by HPLC device.
In this study, it is determined that pistachio is rich in flavonoid. Other results indicated that CCl4 application significantly increased MDA and GSSG contents the most significantly (p<0.001). However, the GSH level decreased the more significantly (p<0.01). In the groups given PV extract against CCl4, it was found that the GSH, GSSG levels increased and MDA level reduced in S. cerevisiae cell (p<0.01, p<0.001). According to the results in comparison to control group, the activity of SOD GST, GSH, CAT and GSH-Px were decreased significantly with CCl4 treatment (p<0.05; p<0.01). In our data in comparison to control group, the activity of SOD, GST, GSH, CAT and GSH-Px were increased significantly in PV2+CCl4 and PV4+CCl4 groups with treatment PV extract (p<0.01;p<0.001).
Our study shows that PV is very rich in flavonoids. As a result, PV extract has a role in promoting cell growth as well as reducing oxidative damage in S. cerevisiae culture. In particular, the results of the antioxidant defense system will be a source for similar studies on other living models.
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