Determination of DNA Damage Caused by Food Additives Using Comet Assay Method Food Additives DNA damage via comet assay

Main Article Content

Mine DOSAY-AKBULUT

Keywords

DNA Damage, Comet, Food Additives and Genotoxic

Abstract

Additives have been used to improve specific characteristics of food products. As a result of the rapid increase in the use of chemical substances such as food additives in every area, it has become very important to determine whether these chemicals have negative effects on the genetic structure of living things. Food additives are substances and used to preserve flavor or enhance its taste, appearance, or other qualities of foods. Especially; food preservatives within food additives are in use to protect the food against to microorganisms negative effect. The risk assessing from food additives to human health carried out by WHO, in cooperation with FAO. However, the increased consumption of food additives may result to toxic reactions. It was indicated by different studies that some food additives have genotoxic and carcinogenic effects in different test organisms including plants, bacteria, human lymphocytes and in different organs, mice and rats. The effects of genotoxic and cytotoxic agents on living cells can be determined bu using comet assay anaysis. In recent years, the comet assay method has been widely preferred because of its advantageous, precise and fast results. Becasue of being carcinogenics possibility it should be more concious and be more carefull in taking food additives to our life.

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References

1-Boğa A, Binokay S. Gıda Katkı Maddeleri ve Sağlığımıza Etkileri. Arşiv 2010; 19: 141.
2-Yüzbaşıoğlu D, Zengin N, Ünal F. Gıda koruyucuları ve Genotoksisite Testleri. Gıda 2014; 39 (3): 179-186.
3-Özel B, Şimşek Ö, Akçelik M, Saris PEJ. 2018. Innovative approaches to nisin production. Appl Microbiol Biotechnol 2018; 102(15): 6299-6307.
4-http://www.apelasyon.com/Yazi/321-gida-katki-maddeleri-3---koruyucular-antimikrobiyal-katki-maddeleri-Eylül 2015.
5-Şen S, Aksoy H,&Yılmaz S. Gıda katkı maddelerinin genotoksik, karsinojenik potansiyeli ve insan sağlığı üzerindeki diğer etkileri. J Hum Sci 2017; 14(4): 3093-3108.
6-Altuntaş GE, Ayhan K. Süt ve Süt Ürünlerinde Bakteriyosinlerin Kullanımı. Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi 2010; 16 (1): 113-120.
7-Sasaki YF, Kawaguchi S, Kamaya A, et al. The comet assay with 8 mouse organs: results with 39 currently used food additives. Mut Res 2002; 519: 103-119.
8-Atrı R, Sıngh A, Mathur N, Verma A. Genotoxıc Effect of Food Addıtıves and Food Products: A Revıew. Int J Chem 2013; 11(4): 1753-1768.
9-Dıncer Y, Kankaya S. DNA Hasarının Belirlenmesinde Comet Assay. Türkiye Klinikleri 2010; 30: 1365-73.
10-Erısmıs U, Cıgercı HI, Konuk M. 2012. Comet Assay in Amphibian Biomonitoring. 21. Ulusal Biyoloji Kongresi, Ege Üniversitesi, İzmir, Türkiye.
11-Sıngh NP, Mccoy MT, Tıce RR, Schneıder EL. “A simple technique for quantitation of low levels of DNA damage in individual cells”. Exp Cell Res 1988; 175: 184-191.
12-Tıce RR, Agurell E, Anderson D, Burlınson B, Hartmann A, Kobayashı H. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000; 35: 206–21.
13-Faırbaırn DW, Olıve PL, O'neıl KL. The comet assay: a comprehensive review. Mutat Res 1995; 339: 37-59.
14-Ostling O, Johanson KJ. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 1984; 123: 291-8.
15-Collins AR. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 2004; 26: 249–261.
16-Tıce RR, Andrews PW, Hıraı O, Sıngh NP. The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in individual cells. Adv Exp Med Biol 1991; 283: 157-164.
17-Brendler-Schwaab S, Hartmann A, Pfuhler S, Speit G. The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 2005; 20(4): 245-54.
18-https://www.sigmaaldrich.com/life-science/cell-biology/cancer-research/learning-center/cancer-research-protocols/comet-assay.html
19-Anderson D, Dhawan A, Weı T, Plewa MJ. An investigation of bone marrow and testcular cells invivo using the comet assay. Mutat Res 1996; 370: 159-174.
20-Güleç S. 2019. Hibernasyonda “pelophylax caralıtanus” (amphibia: anura)’da DNA Hasarının Araştırılması, Msc Thesis, Afyon Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü Afyon.
21-Yilmaz S, Ünal F, Yüzbaşıoğlu D, Çelik M. DNA damage in human lymphocytes exposed to four food additives in vitro. Toxicol Ind Health 2014; 30(10): 926–937.
22-Shimada C, Kano K, Sasaki Yu F, Sato I, Tsuda S. Differential colon DNA damage induced by azo food additives between rats and mice. J Toxicol Sci 2010; 35(4): 547-554.
23-Ishidate M, Sofuni T, Jr Yoshikawa K, et al. Primary mutagenicity screening of food additives currently used in Japan. Food Chem Toxicol 1984; 22: 623–636.
24-Gürsoy S. 2002. Besinlerde katkı maddelerinin kullanımı ve sitrik asit toksisitesi. MSc Thesis, Trakya Üniversitesi Fen Bilimleri Enstitüsü, Edirne.
25-Khayyat LI, Essawy AE, Sorour JM, Soffar A. Sunset Yellow and Allura Red modulate Bcl2 and COX2 expression levels and confer oxidative stress-mediated renal and hepatic toxicity in male rats. Peer J 2018; 6:e5689.
26-Pandir D. DNA damage in human germ cell exposed to the some food additives in vitro. Cytotechnolog 2016; 68(4): 725–733.
27-Hassan GM. Effects of some synthetic coloring additives on DNA damage and chromosomal aberrations of rats. Arab J Biotechnol 2010; 13 (1):13-24.
28-Abo-El-Sooud K, Hashem MM, Badr YA, et al. Assessment of hepato-renal damage and genotoxicity induced by long-term exposure to five permitted food additives in rats. Environ Sci Pollut Res Int 2018; 25(26): 26341-26350.
29-Sarikaya R, Cakir S. Genotoxicity Testing of Four Food Preservatives and their Combinations in the Drosophila Wing Spot Test. Environ Toxicol Pharmacol 2005; (3): 424- 430.
30-Demir E, Kocaoglu S, Kaya B. Genotoxicity Testing of Four Benzyl Derivatives in the Drosophila Wing Spot Test. Food ChemToxicol 2008; 46(3): 1034-1041.
31-Llana-Ruiz-Cabello M, Puerto M, Maisanaba S, Guzmán-Guillén R, Pichardo S, Cameán AM. Use of micronucleus and comet assay to evaluate evaluate the genotoxicity of oregano essential oil (Origanum vulgare l. Virens) in rats orally exposed for 90 days. J Toxicol Environ Health A 2018; 81(12): 525-533.
32-Møller P1. Genotoxicity of environmental agents assessed by the alkaline comet assay. Basic Clin Pharmacol Toxicol 2005; 96 (1):1-42.
33-Abd-Elhakim YM, Anwar A, Hashem MM, Moustafa GG, Abo-El-Sooud K. Sodium Acetate, Sodium Acid Pyrophosphate, and Citric Acid Impacts on Isolated Peripheral Lymphocyte Viability, Proliferation, and DNA Damage. J Biochem Mol Toxicol 2018; 32(8):e22171. doi: 10.1002/jbt.22171.
34-Hobbs CA, Taylor SV, Beevers C, et al. Genotoxicity assessment of the flavouring agent, perillaldehyde. Food Chem Toxicol 2016; 97:232-242. doi: 10.1016/j.fct.2016.08.029.
35-Kitamoto S, Matsuyama R, Uematsu Y, et al. Genotoxicity evaluation of benzene, di(2-ethylhexyl) phthalate, and trisodium ethylenediamine tetraacetic acid monohydrate using a combined rat comet/micronucleus assays. Mutat Res Genet Toxicol Environ Mutagen 2015; 786-788:137-43. doi: 10.1016/j.mrgentox.2015.05.002.
36-Koç K, Pandir D. All aspect of toxic effect of brilliant blue and sunset yellow in Allium cepa roots. Cytotechnology 2018; 70(1): 449-463. doi: 10.1007/s10616-017-0161-9.
37-Di Mauro MD, Tomasello B, Giardina RC, et al. Sugar and mineral enriched fraction from olive mill wastewater for promising cosmeceutical application: characterization, in vitro and in vivo studies. Food Funct 2017; 8(12):4713-4722. doi: 10.1039/c7fo01363a.
38-Mamur S, Yüzbaşıoğlu D, Unal F, Aksoy H. Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro. Cytotechnology 2012; 64(5):553-62. doi: 10.1007/s10616-012-9434-5.
39-Vollmuth TA. Caramel color safety - An update. Food Chem Toxicol 2018; 111:578-596. doi: 10.1016/j.fct.2017.12.004.
40-Bastaki M, Farrell T, Bhusari S, Pant K, Kulkarni R. Lack of genotoxicity in vivo for food color additive Tartrazine. Food Chem Toxicol 2017; 105:278-284. doi: 10.1016/j.fct.2017.04.034.
41-Kirkland D, Gatehouse D. "Aspartame: A review of genotoxicity data". Food Chem Toxicol 2015; 84:161-8. doi: 10.1016/j.fct.2015.08.021.
42-Ataseven N, Yüzbaşıoğlu D, Keskin AÇ, Ünal F. Genotoxicity of monosodium glutamate. Food Chem Toxicol 2016; 91:8-18. doi: 10.1016/j.fct.2016.02.021.
43-Schwartz GR. Aspartame and breast and other cancers. West J Med 1999; 171(5-6): 300-1.
44-Wolff S, Rodin B. Saccharin-induced sister chromatid exchanges in Chinese hamster and human cells. Science 1978; 200: 543-545.
45-Peycheva E, Alexandrova R, Miloshev G. Application of the yeast comet assay in testing of food additives forgenotoxicity. LWT- Food Sci Technol 2014; 59: 510-517.