Therapeutic efficacy of water extract of Oyster Mushroom in streptozotocin induced diabetic Wistar rats
Main Article Content
Keywords
Streptozotocin, Wistar rats, type -2 diabetes, Oyster Mushroom
Abstract
The present study was investigated for the quantitative analysis of phytochemicals, antioxidants, α-amylase inhibitory activity and antidiabetic properties of aqueous extract of oyster mushroom. Quantitative analysis of aqueous extract shows phenolics, flavonoids, tannins, alkaloids and saponins as bioactive compounds. Furthermore, using DPPH radical scavenging activity, hydrogen peroxide scavenging activity, total antioxidant capacity, and anti-haemolytic activity were found to be 54.41±1.18, 11.87±1.21, 29.23±3.12, and 14.42±4.89 (activity measured in % inhibition). In the next phase of our study, we evaluated the α-amylase inhibitory activity of the extract in a dose dependent manner and found the inhibition of (89.96±4.6 % at 1000 μg/ml). Finally, oyster mushroom extract was administered to diabetic rats (200 mg/kg) for 21 days to examine its anti-hyperglycemic, anti-hyperlipidemic and anti-oxidative properties. A significant reduction in triglyceride, total cholesterol, and low-density lipoprotein-cholesterol (LDL-C) was demonstrated by the extract. Furthermore, oyster mushroom aqueous extract improved high density lipoprotein- cholesterol (HDL-C) levels and plasma insulin levels.
References
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25. Bowers LD, Wong ET. Kinetic serum creatinine assays. II. A critical evaluation and review. Clinical chemistry 1980; 26: 555-61.
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27. Fossati P, Prencipe L, Berti G. Use of 3, 5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clinical chemistry 1980; 26: 227-31.
28. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology 1957; 28: 56-63.
29. Fuehr J. Bilirubin determination in the serum according to the method of l. jendrassik, ra cleghorn and p. grof. Medizinische Monatsschrift 1964; 18: 183-4.
30. Gl E. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82: 70-7.
31. Winterbourn CC, Hawkins RE, Brian M, Carrell R. The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine 1975; 85: 337-41.
32. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry 1979; 95: 351-8.
33. Sinha AK. Colorimetric assay of catalase. Analytical biochemistry 1972; 47: 389-94.
34. Lowry O, Rosebrough N, Farr AL, Randall R. Protein measurement with the Folin phenol reagent. Journal of biological chemistry 1951; 193: 265-75.
35. Soetan K. Pharmacological and other beneficial effects of antinutritional factors in plants-A review. African journal of Biotechnology 2008; 7.
36. Baskaran K, Ahamath BK, Shanmugasundaram KR, Shanmugasundaram E. Antidiabetic effect of a leaf extract from Gymnema sylvestre in non-insulin-dependent diabetes mellitus patients. Journal of ethnopharmacology 1990; 30: 295-305.
37. Prabu M, Kumuthakalavalli R. Antidiabetic potential of the oyster mushroom Pleurotus florida (Mont.) Singer. Int J Curr Pharm Res 2017; 9: 51-4.
38. Mohamed J, Shing SW, Idris MHM, Budin SB, Zainalabidin S. The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes. Clinics 2013; 68: 1358-63.
39. Karim R, Rahman F, Rahman R et al. Hypoglycemic and antidyslipidemic potential of Pleurotus ostreatus in streptozotocin-induced diabetic rats. J Adv Biotechnol Exp Ther 2020; 3: 49-55.
40. Sung M-H, Liao F-H, Chien Y-W. Medium-chain triglycerides lower blood lipids and body weight in streptozotocin-induced type 2 diabetes rats. Nutrients 2018; 10: 963.
41. Pisoschi AM, Cheregi MC, Danet AF. Total antioxidant capacity of some commercial fruit juices: electrochemical and spectrophotometrical approaches. Molecules 2009; 14: 480-93.
42. Karadeniz F, Burdurlu HS, Koca N, Soyer Y. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turkish Journal of Agriculture and Forestry 2005; 29: 297-303.
43. Vishwakarma P, Singh P, Tripathi N. In-vitro antioxidant activity and nutritional value of four wild oyster mushroom collected from North-Eastern Part of Uttar Pradesh. Mycosphere 2017; 8: 592-602.
44. Sarker MMR. Antihyperglycemic, insulin-sensitivity and anti-hyperlipidemic potential of Ganoderma lucidum, a dietary mushroom, on alloxan-and glucocorticoid-induced diabetic Long-Evans rats. Functional Foods in Health and Disease 2015; 5: 450-66.
45. Dirir AM, Daou M, Yousef AF, Yousef LF. A review of alpha-glucosidase inhibitors from plants as potential candidates for the treatment of type-2 diabetes. Phytochem Rev 2022; 21: 1049-79.
46. Oboh G, Isaac AT, Akinyemi AJ, Ajani RA. Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside induced lipid peroxidation in rats' pancreas by phenolic extracts of avocado pear leaves and fruit. Int J Biomed Sci 2014; 10: 208-16.
47. Van De Laar FA, Lucassen PL, Akkermans RP, van de Lisdonk EH, Rutten GE, van Weel C. α-Glucosidase inhibitors for patients with type 2 diabetes: results from a Cochrane systematic review and meta-analysis. Diabetes care 2005; 28: 154-63.
2. Chawla A, Chawla R, Jaggi S. Microvasular and macrovascular complications in diabetes mellitus: distinct or continuum? Indian journal of endocrinology and metabolism 2016; 20: 546.
3. Yadav D, Mishra M, Tiwari A, Bisen PS, Goswamy HM, Prasad G. Prevalence of dyslipidemia and hypertension in Indian type 2 diabetic patients with metabolic syndrome and its clinical significance. Osong public health and research perspectives 2014; 5: 169-75.
4. Atlas D. International diabetes federation. IDF Diabetes Atlas, 7th edn Brussels, Belgium: International Diabetes Federation 2015; 33.
5. Kumar D, Bajaj S, Mehrotra R. Knowledge, attitude and practice of complementary and alternative medicines for diabetes. Public health 2006; 120: 705-11.
6. Brundisini F, Vanstone M, Hulan D, DeJean D, Giacomini M. Type 2 diabetes patients’ and providers’ differing perspectives on medication nonadherence: a qualitative meta-synthesis. BMC health services research 2015; 15: 1-23.
7. Prakash O, Kumar R, Srivastava R, Tripathi P, Mishra S, Ajeet A. Plants explored with anti-diabetic properties: A review. Am J Pharmacol Sci 2015; 3: 55-66.
8. Koh JH, Lee ES, Hyun M et al. Taurine alleviates the progression of diabetic nephropathy in type 2 diabetic rat model. International Journal of Endocrinology 2014; 2014.
9. Mahajan S, Chauhan P, Mishra M, Yadav D, Debnath M, Prasad G. Antidiabetic potential of eugenia jambolana ethanolic seed extract: Effect on antihyperlipidemic and antioxidant in experimental streptozotocin-induced diabetic rats. Adv Complement Alt Med 2018; 2: 1-9.
10. Chen J, Mao D, Yong Y, Li J, Wei H, Lu L. Hepatoprotective and hypolipidemic effects of water-soluble polysaccharidic extract of Pleurotus eryngii. Food chemistry 2012; 130: 687-94.
11. Chen G, Luo Y-C, Ji B-P et al. Hypocholesterolemic effects of Auricularia auricula ethanol extract in ICR mice fed a cholesterol-enriched diet. Journal of food science and technology 2011; 48: 692-8.
12. Liu Y-T, Sun J, Luo Z-Y et al. Chemical composition of five wild edible mushrooms collected from Southwest China and their antihyperglycemic and antioxidant activity. Food and Chemical Toxicology 2012; 50: 1238-44.
13. Colak A, Faiz O, Sesli E. Nutritional composition of some wild edible mushrooms. Turkish Journal of Biochemistry 2009; 34: 25-31.
14. Assemie A, Abaya G. The Effect of Edible Mushroom on Health and Their Biochemistry. Int J Microbiol 2022; 2022: 8744788.
15. Ng SH, Mohd Zain MS, Zakaria F, Wan Ishak WR, Wan Ahmad WAN. Hypoglycemic and antidiabetic effect of Pleurotus sajor-caju aqueous extract in normal and streptozotocin-induced diabetic rats. BioMed research international 2015; 2015.
16. Malik CP, Singh M. Plant enzymology and histo-enzymology. 1980.
17. Swain T, Hillis W. The phenolic constituents of Prunus domestica. I.—The quantitative analysis of phenolic constituents. Journal of the Science of Food and Agriculture 1959; 10: 63-8.
18. Harborne J. Methods of plant analysis. Phytochemical methods: a guide to modern techniques of plant analysis: Springer; 1984. p. 1-36.
19. Brunner J. Direct spectrophotometric determination of saponin. Analytical chemistry 1984; 34: 1314-26.
20. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry 1959; 31: 426-8.
21. Shabbir M, Khan MR, Saeed N. Assessment of phytochemicals, antioxidant, anti-lipid peroxidation and anti-hemolytic activity of extract and various fractions of Maytenus royleanus leaves. BMC complementary and alternative medicine 2013; 13: 1-13.
22. Stockbridge H, Hardy RI, Glueck CJ. Public cholesterol screening: motivation for participation, follow-up outcome, self-knowledge, and coronary heart disease risk factor intervention. The Journal of laboratory and clinical medicine 1989; 114: 142-51.
23. Fossati P, Prencipe L. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical chemistry 1982; 28: 2077-80.
24. Lopes-Virella MF, Stone P, Ellis S, Colwell JA. Cholesterol determination in high-density lipoproteins separated by three different methods. Clinical chemistry 1977; 23: 882-4.
25. Bowers LD, Wong ET. Kinetic serum creatinine assays. II. A critical evaluation and review. Clinical chemistry 1980; 26: 555-61.
26. Fawcett J, Scott J. A rapid and precise method for the determination of urea. Journal of clinical pathology 1960; 13: 156-9.
27. Fossati P, Prencipe L, Berti G. Use of 3, 5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. Clinical chemistry 1980; 26: 227-31.
28. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American journal of clinical pathology 1957; 28: 56-63.
29. Fuehr J. Bilirubin determination in the serum according to the method of l. jendrassik, ra cleghorn and p. grof. Medizinische Monatsschrift 1964; 18: 183-4.
30. Gl E. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82: 70-7.
31. Winterbourn CC, Hawkins RE, Brian M, Carrell R. The estimation of red cell superoxide dismutase activity. The Journal of laboratory and clinical medicine 1975; 85: 337-41.
32. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry 1979; 95: 351-8.
33. Sinha AK. Colorimetric assay of catalase. Analytical biochemistry 1972; 47: 389-94.
34. Lowry O, Rosebrough N, Farr AL, Randall R. Protein measurement with the Folin phenol reagent. Journal of biological chemistry 1951; 193: 265-75.
35. Soetan K. Pharmacological and other beneficial effects of antinutritional factors in plants-A review. African journal of Biotechnology 2008; 7.
36. Baskaran K, Ahamath BK, Shanmugasundaram KR, Shanmugasundaram E. Antidiabetic effect of a leaf extract from Gymnema sylvestre in non-insulin-dependent diabetes mellitus patients. Journal of ethnopharmacology 1990; 30: 295-305.
37. Prabu M, Kumuthakalavalli R. Antidiabetic potential of the oyster mushroom Pleurotus florida (Mont.) Singer. Int J Curr Pharm Res 2017; 9: 51-4.
38. Mohamed J, Shing SW, Idris MHM, Budin SB, Zainalabidin S. The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes. Clinics 2013; 68: 1358-63.
39. Karim R, Rahman F, Rahman R et al. Hypoglycemic and antidyslipidemic potential of Pleurotus ostreatus in streptozotocin-induced diabetic rats. J Adv Biotechnol Exp Ther 2020; 3: 49-55.
40. Sung M-H, Liao F-H, Chien Y-W. Medium-chain triglycerides lower blood lipids and body weight in streptozotocin-induced type 2 diabetes rats. Nutrients 2018; 10: 963.
41. Pisoschi AM, Cheregi MC, Danet AF. Total antioxidant capacity of some commercial fruit juices: electrochemical and spectrophotometrical approaches. Molecules 2009; 14: 480-93.
42. Karadeniz F, Burdurlu HS, Koca N, Soyer Y. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turkish Journal of Agriculture and Forestry 2005; 29: 297-303.
43. Vishwakarma P, Singh P, Tripathi N. In-vitro antioxidant activity and nutritional value of four wild oyster mushroom collected from North-Eastern Part of Uttar Pradesh. Mycosphere 2017; 8: 592-602.
44. Sarker MMR. Antihyperglycemic, insulin-sensitivity and anti-hyperlipidemic potential of Ganoderma lucidum, a dietary mushroom, on alloxan-and glucocorticoid-induced diabetic Long-Evans rats. Functional Foods in Health and Disease 2015; 5: 450-66.
45. Dirir AM, Daou M, Yousef AF, Yousef LF. A review of alpha-glucosidase inhibitors from plants as potential candidates for the treatment of type-2 diabetes. Phytochem Rev 2022; 21: 1049-79.
46. Oboh G, Isaac AT, Akinyemi AJ, Ajani RA. Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside induced lipid peroxidation in rats' pancreas by phenolic extracts of avocado pear leaves and fruit. Int J Biomed Sci 2014; 10: 208-16.
47. Van De Laar FA, Lucassen PL, Akkermans RP, van de Lisdonk EH, Rutten GE, van Weel C. α-Glucosidase inhibitors for patients with type 2 diabetes: results from a Cochrane systematic review and meta-analysis. Diabetes care 2005; 28: 154-63.
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Ghatak M, Yadav D, Singh PK, Kim S. Therapeutic efficacy of water extract of Oyster Mushroom in streptozotocin induced diabetic Wistar rats. Progr Nutr [Internet]. 2024 Dec. 19 [cited 2024 Dec. 19];26(3-4):e2024020. Available from: https://mattioli1885journals.com/index.php/progressinnutrition/article/view/15313
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
1.
Ghatak M, Yadav D, Singh PK, Kim S. Therapeutic efficacy of water extract of Oyster Mushroom in streptozotocin induced diabetic Wistar rats. Progr Nutr [Internet]. 2024 Dec. 19 [cited 2024 Dec. 19];26(3-4):e2024020. Available from: https://mattioli1885journals.com/index.php/progressinnutrition/article/view/15313
