Determination of Preoperative and Postoperative Serum Leptin, Ghrelin, Insulin, and Irisin Hormone Levels in Left Abomasum Displacement

Main Article Content

Hale Ergin Eğritağ
Tülay Büyükoğlu
Kürşad Yiğitarslan

Keywords

Hormonal regulation, left displacement abomasum, metabolism, negative energy balance

Abstract

Background and aim: Left Displacement Abomasum (LDA) is a metabolic disorder associated with negative energy balance, especially in dairy cattle, and might cause economic losses. This study, it was aimed to evaluate the serum levels of insulin, leptin, ghrelin, and irisin hormones responsible for energy metabolism before and after the operation in Holstein cattle with LDA. Methods: For this purpose, cattle were divided into three groups (Group 1: Healthy, Group 2: Pre-operative, Group 3: Post-operative) with 8 animals in each group, and blood samples were collected. In serum samples, glucose, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHBA), total protein, triglyceride, total cholesterol, and calcium levels were measured with an autoanalyzer. In addition, commercial kits measured serum insulin, leptin, ghrelin, and irisin hormone levels with the EIA method. Results: Preoperative serum glucose, NEFA, and BHBA levels were higher in cattle with LDA, and total protein and triglyceride levels were lower than in healthy animals (p<0.05). In cattle with LDA after the operation, these values ​​were similar to those of healthy animals. In hormone analysis, ghrelin, insulin, and irisin were found to be higher in animals with LDA, while leptin levels were found to be decreased (p<0.05). After the operation, the insulin levels of the cattle with LDA decreased below the pre-operative values; It was observed that leptin and irisin levels were increased (p<0.05). Conclusions: In cattle with LDA, increased ghrelin levels may be due to anorexic conditions; Increased insulin levels may be due to stress and negative energy balance. Decreased serum insulin levels in cattle after the operation suggested that it may be related to the insulin resistance state caused by the left displacement abomasum. Increased serum leptin can be considered an acute phase response to leptin, and an increase in serum irisin can be a marker of pain and stress.

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References

1. Zerbin I, Lehner S, Distl O. Genetics of bovine abomasal displacement. Vet J 2015; 204: 17–22.
2. McArt JA, Nydam DV, Overton MW. 2015. Hyperketonemia in early lactation dairy cattle: a deterministic estimate of component and total cost per case. J Dairy Sci 98(3):2043–2054.
3. Caixeta LS, Herman JA, Johnson GW, McArt J. 2018. Herd-level monitoring and prevention of displaced abomasum in dairy cattle. Vet Clin North Am Food Anim Pract 34(1):83–99.
4. Constable PD, Miller GY, Hoffsis GF, Hull BL, Rings DM. Risk factors for abomasal volvulus and left abomasal displacement in cattle. Am J Vet Res 1992; 7: 1184–1192.
5. Aydın S, Özkan Y, Caylak E, Aydın S. Ghrelin ve biyokimyasal fonksiyonları Türkiye Klinikleri J Med Sci 2006; 26: 272-283.
6. Ozturk AS, Guzel M, Askar, TK, Aytekin, I. Evaluation of the hormones responsible for the gastrointestinal motility in cattle with displacement of the abomasum; ghrelin, motilin and gastrin Vet Rec 2013; 172: 636.
7. Kadokawa H, Blache D, Yamada Y, Martin GB. Relationships between changes in plasma concentrations of leptin before and after parturition and the timing of first post-partum ovulation in high-producing Holstein dairy cows. Reprod Fertil Dev 2000; 12: 405–411.
8. Nkrumah JD, Li C, Yu J, Hansen C, Keisler DH, Moore SS. Polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit. J Anim Sci 2005; 83: 20–28.
9. Chebel RC, Susca F, Santos JEP. Leptin genotype is associated with lactation performance and health of Holstein cows. Journal of Dairy Science 2008; 91: 2893–2900.
10. Boström P, Wu J, Jedrychowski MP, Korde A, et al. A PGC1-[agr]-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature 2012; 48(7382): 463-8
11. Zhang Y, Li R, Meng Y, et al. Irisin stimulates browning of white adipocytes through mitogen-activared protein kinase p38 MAP kinase and ERK MAP kinase signaling. Diabetes 2014; 63(2): 514-25.
12. Yurdakul, İbrahim, and Uğur Aydoğdu. "The right displacement of the abomasum complicated with abomasitis in a calf." Ankara Univ Vet Fak Derg 2018; 65 (4): 433-437.
13. Wilson DG. Management of abomasal displacement. Large Anim Vet Rounds 2008; 8(8): 1-6.
14. Sen, Ismail, Thomas Wittek, and Hasan Guzelbektes. Metabolic indicators and risk factors of left displaced abomasum in dairy cattle. Eurasian J Vet Sci 31.2 (2015): 63-69.
15. Şahal M, Öcal N, Özgencil E, Besaltı Ö, Tanyel B. Abomasum deplasmanlı süt ineklerinde kan serumu, rumen sıvısı, tükrük ve idrarda biyokimyasal incelemeler. Ankara Üniv. Vet Fak Derg 1997; 43: 1-6.
16. Vlamick L, Steenhaut M, Gasthuys F, Martens A, Desmet P, Vanden Abeele K, Moor D. Omentopeksy for correction of left displaced abomasum: Outcome results in 53 cattle. Vlaams Diergeneeskunding Tijdschrift, 1998; 67: 118-122.
17. Delgado-Lecaroz R, Warnick LD, Guard CL, Smith MC, Barry DA. Cross-sectional study of the association of abomasal displacement or volvulus with serum electrolyte and mineral concentrations in dairy cows. Can Vet J 2000; 41(4):301-305.
18. Katoh, N., Nakagawa-Ueta, H. Concentrations of apolipoprotein C-III in healthy cows during the prepartum period and cows with milk fever. J Vet Med Sci 2001; 63(6): 597-601
19. Ismael M, Elshahawy I, Hassan I. New Insights on Left Displaced Abomasum in Dairy Cows. Alex J Vet Sci 2018; 56: 27.
20. Mahmoud A.A., Nahed S.S., Tamer S.A., Hany G.K. Evaluation of Clinical, Serum Biochemical and Oxidant-antioxidant Profiles in Dairy Cows with Left Abomasal Displacement. Asian J of Anim. and Vet Advan 2016; 11: 242-247
21. Herdt TH. Ruminant adaptation to negative energy balance. Vet Clin North Am Food Anim Pract 2000;16: 215-230.
22. LeBlanc SJ, Leslie KE, Duffield TF. Metabolic predictors of displaced abomasum in dairy cattle. J Dairy Sci 2005; 88: 159–170.
23. Kalaitzakis E, Roubies N, Panousis N, et al. Evaluation of ornithine carbamoyl transferase and other serum and liver derived analytes in diagnosis of fatty liver and postsurgical outcome of left-displaced abomasum in dairy cows. JAVMA 2006; 229: 1463-1471
24. Markiewicz H, Gehrke M, Malinowski E. Selected biochemical blood compounds in cows with abomasum displacement. Pol J Vet Sci 2009; 2: 515-518.
25. Van Winden SCL, Kuiper R. Left Displacement of the Abomasum in Dairy Cattle: Recent Developments in Epidemiological and Etiological Aspects. Vet Res 2003; 34: 47-56.
26. Zadnik T. A comparative study of the hematobiochemical parameters between clinically healthy cows and cows with displacement of the abomasum. Acta Vet- Beograd 2003; 53: 297-309.
27. Holtenıus K, Jacobbson So, Holtenıus P. Effects of intravenous infusion of glucose and pancreatic glucagon on abomasal function in dairy cows. Acta Vet. Scand. 1998; 39(2): 291-300.
28. Holtenıus K, Sternbauer K, Holtenıus P. The effect of the plasma glucose level on the abomasal function in dairy cows. J Anim Sci 2000; 78:1930-1935.
29. Pravettoni D, Doll K, Hummel M, Cavallone E, Re M, Belloli AG. Insulin resistance and abomasal motility disorders in cow detected by use of abomasoduodenal electromyography after surgical correction of left displaced abomasum. Am J Vet Res 2004; 65: 1319–1324.
30. Donatelli F, Nafi M, Pietropaoli L, et al. Postoperative insulin secretion is decreased in patients with preoperative insulin resistance. Acta Anaesthesiol Scand 2019; 63(2): 232–239.
31. Liefers SC, Veerkamp RF, Te Pas MFW, Chilliard Y,Van der Lende T. Genetics and physiology of leptin inperiparturient dairy cows. Domes Anim Endocrinol 2005; 29: 227-238.
32. Zhang Y, Chua S Jr. Leptin Function and Regulation. Compr Physiol 2017;8(1):351-369.
33. Williams GL, Amstalden M, Garcia MR, et al. Leptin and itsrole in the central regulation of reproduciton in cattle. Domes Anim Endocrinol 2002; 23339-23349.
34. Abizaid A, Kyriazis D, Woodside K. Effects of leptinadministration on lactational infertility in food-restricted rats depend on milk delivery. Am J Physiol 2004; 286: 17-225.
35. D'Occhio MJ, Baruselli PS, Campanile G. Influence of nutrition, body condition, and metabolic status on reproduction in female beef cattle: A review. Theriogenology 2019; 125: 277-284.
36. Meikle A, Kulcsar M, Chilliard Y, Febel H, Delavaud C, Caves-Tany D, Chilibroste P. Effects of parity and body condition at parturition on endocrine and reproductive parameters of the cow. Reprod 2004; 127: 727-737.
37. Maruna P, Gürlich R, Fried Met al. Leptin as an acute phase reactant after non-adjustable laparoscopic gastric banding. Obes Surg 2001; 11(5): 609-614.
38. Kojima M, Hosoda H, Date Y, et al. Ghrelin is a growth hormone-releasing acylated peptide from stomach. Nature 1999; 402(6762): 656–660.
39. Trudel L, Tomasetto C, Rio MC, et al. Ghrelin/motilin-related peptide is a potent prokinetic to reverse gastric postoperative ileus in rat. Am J Physiol Gastrointest 2002; 282(6): G948–G952.
40. Dornonville de la Cour C, Lindström E, Norlén P, Håkanson R. Ghrelin stimulates gastric emptying but is without effect on acid secretion and gastric endocrine cells. Regul Pept 2004; 120(1-3): 23–32
41. Itoh F, Komatsu T, Yonai M, et al. GH secretory responses to ghrelin and GHRH in growing and lactating dairy cattle. Domest Anim Endocrinol 2004; 28: 34-45.
42. Iqbal J, Kurose Y, Canny B, Clarke IJ. Effects of Central Infusion of Ghrelin on Food Intake and Plasma Levels of Growth Hormone, Luteinizing Hormone, Prolactin, and Cortisol Secretion in Sheep. endocrinol 2006; 147: 510-519.
43. Hayashida T, Murakami K, Mogic K, et al. Ghrelin in domestic animals: distribution in stomach and its possible role. Domestic Animal Endocrinology 2001; 21: 17-24.
44. Arıkan F, Uysal H. Sağlıkta ve hastalıkta ghrelinin rolü. Lalahan Hay Arast Enst Derg 2011; 51: 41-53
45. Wu S, Hassan FU, Luo Y, et al. Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors. Biology 2021;10(11): 1207.
46. Sarac F, Buyukbese Sarsu, S, Yeniocak S, et al. The Diagnostic Value of Irisinin Pediatric Patients with Acute Abdominal Pain. Emerg Med In. 2018; 3296535.