Detection of a novel genetic variant of the Myf5 gene in Turkish Anatolian Water Buffalo
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Keywords
Anatolian Buffalo, Myf5, sequencing, SNPs
Abstract
Myf5 gene belongs to myogenic regulatory factors (MRFs) families and has an active role in muscle differentiation and the development of skeletal muscle cells. The study aimed to detect genetic variants of the Myf5 gene in Anatolian buffaloes. PCR-RFLP and DNA sequencing methods were used to detect SNP regions of the Myf5 gene. Seven restriction enzymes were used to identify the polymorphic regions. Six enzymes (RsaI, SspI, DraI, HinfI, PstI, and EcoRI) showed monomorphic regions and only BsuRI enzyme showed a polymorphic region in the intron 2 site. It was detected a novel SNP in the C14553A site in the second intron of the Myf5 gene in Anatolian buffaloes. These novel findings may be used as a genetic marker and contribute to the performance of buffaloes in the future.
References
2. Khan S, Qureshi M S, Ahmed I, Shah S M. Milk composition and yield changes with advancing pregnancy in dairy buffaloes (Bubalus bubalis). Turk. J. Vet. Anim. Sci 2011; 35: 375-380.
3. Soysal M, Ozkan E, Kok S, et al. Genetic characterization of indigenous anatolian water buffalo breed using microsatellite dna markers. Ital. J. Anim. Sci 2007; 6: 409-412.
4. FAO, 2021. Food and Agricultural Organization of the United Nations, Buffaloes. Available on: http://www.fao.org/dairy-production-products/production/dairy-animals/buffaloes/en/.
5. Değirmencioğlu T. Possibilities to Reduce Thermal Stress in Water Buffalo [Bubalus bubalis (Linnaeus, 1758)]. AARI 2020; 30: 117-123.
6. Karlı B, Gül M, Akpınar M G, Taşcıoğlu Y, Bozkurt Y, Şirikçi B S. Analysis of economic structure in water buffalo breeding by geographical regions in Turkey. Buffalo Bull 2021; 40: 135-150.
7. Kelgökmen İ, Ünal N. Some morphometric traits of Anatolian Buffaloes. Lalahan Hay. Araşt. Enst. Derg 2015; 55: 50-55.
8. Aytekin I, Bayraktar M. Bovine SMO gene polymorphism in Anatolian Black Cattle and Anatolian Water Buffaloes. Adü Ziraat Derg 2021; 18: 29-32.
9. Aytekin İ, Bayraktar M, Sakar Ç M, Ünal İ. Association between MYLK4 gene polymorphism and growth traits at different age stages in Anatolian black cattle. Anim. Biotechnol 2020; 31: 555-560.
10. Ogorevc J, Kunej T, Razpet A, Dovc P. Database of cattle candidate genes and genetic markers for milk production and mastitis. Anim. Genet 2009; 40: 832-851.
11. Aytekin I, Boztepe S. Associations of Pit-1 gene polymorphism with milk yield and composition traits in brown swiss cattle. J Anim Plant Sci 2013; 23: 1281-1289.
12. Aytekin I, Ozdil F, Zulkadir U, Boztepe S, Sariyel V. Evaluation of ISSR markers for genetic diversity analysis in Anatolian water buffaloes. J. Sci. Food Agric 2011; 91: 1957-1962.
13. Rezende F, Ferraz J, Eler J, Silva R, Mattos E, Ibáñez-Escriche N. Study of using marker assisted selection on a beef cattle breeding program by model comparison. Livest. Sci 2012; 147: 40-48.
14. Bentzinger C F, Wang Y X, Rudnicki M A. Building muscle: molecular regulation of myogenesis. Cold Spring Harb. Perspect. Biol 2012; 4: 1-16.
15. Genxi Z, Ying T, Tao Z, Jinyu W, Yongjuan W. Expression profiles and association analysis with growth traits of the MyoG and Myf5 genes in the Jinghai yellow chicken. Mol. Biol. Rep 2014; 41: 7331-7338.
16. Kunhareang S, Zhou H, Hickford J G. Allelic variation in the porcine MYF5 gene detected by PCR–SSCP. Mol. Biotechnol 2009; 41: 208-212.
17. Fadhil M, Zülkadir U. Association between polymorphisms of Myf5, MSTN and CAST genes and fattening performance in Brown Swiss and Holstein cattle breeds. Anim. Biotechnol 2021; 32: 121-129.
18. Saputra E, Ulum M, Jakaria J. Association of SNP g. 643G> A of MYF5 gene polymorphism with body weight and body measurements in Bali cattle. J. Indones. Trop. Anim. Agric 2020; 45: 1-6.
19. Ujan J, Zan L, Ujan S, Wang H, 2011. Association between polymorphism of MYF5 gene with meat quality traits in indigenous Chinese cattle breeds. International Conference on Asia Agriculture and Animal. IACSIT, Singapore, pp. 50-55.
20. Wang J, Hu Y, Elzo M A, et al. Genetic effect of Myf5 gene in rabbit meat quality traits. J. Genet 2017; 96: 673-679.
21. Hernández-Hernández J M, García-González E G, Brun C E, Rudnicki M A. The myogenic regulatory factors, determinants of muscle development, cell identity and regeneration. Semin. Cell Dev. Biol 2017; 72: 10-18.
22. Chung E, Kim W. Association of SNP marker in IGF-I and MYF5 candidate genes with growth traits in Korean cattle. Asian-australas. J. Anim. Sci 2005; 18: 1061-1065.
23. Zhang R, Chen H, Lei C, et al. Association between polymorphisms of MSTN and MYF5 genes and growth traits in three Chinese cattle breeds. Asian-australas. J. Anim. Sci 2007; 20: 1798-1804.
24. Zhao C, Raza S H A, Khan R, et al. Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle. Genomics 2020; 112: 2804-2812.
25. Li C, Basarab J, Snelling W, et al. Assessment of positional candidate genes myf 5 and igf 1 for growth on bovine chromosome 5 in commercial lines of Bos taurus. J. Anim. Sci 2004; 82: 1-7.
26. Bhuiyan M, Kim N, Cho Y, et al. Identification of SNPs in MYOD gene family and their associations with carcass traits in cattle. Livest. Sci 2009; 126: 292-297.
27. Cieslak D, Kuryl J, Kapelanski W, Pierzchala M, Grajewska S, Bocian M. Relationship between genotypes at at MYOG, MYF3 and MYF5 loci and carcass meat and fat deposition traits in pigs. Anim. Sci. Pap. Rep 2002; 20: 79-92.
28. Liu M, Peng J, Xu D, et al. Associations of MYF5 gene polymorphisms with meat quality traits in different domestic pig (Sus scrofa) populations. Genet. Mol 2007; 30: 370-374.
29. Te Pas M, Harders F, Soumillion A, Born L, Buist W. Genetic variation at the porcine MYF-5 gene locus. Lack of association with meat production traits. Mamm. Genome 1999; 10: 123-127.
30. Khang N T K, Ngu N T. Effects of myogenic factor 5 (MYF5) gene on carcass and meat quality of Mong Cai pigs. Thai J. Vet. Med 2013; 43: 213-218.
31. Daldaban F, Arslan K, Aksel E G, Akyüz B. Polymorphism of the STAT5A and MYF-5 genes in Anatolian water buffalo. Turk. J. Vet. Anim. Sci 2020; 44: 284-289.
32. Nasr S, Ateya A, Sadek K, Radwan H. TaqI polymorphism in MYF5gene and its association with body weight in Friesian bull calves. Asian. J. Anim. Vet. Adv 2016; 11: 429-433.
33. Wang J, Zhou H, Forrest R H, et al. Variation in the ovine MYF5 gene and its effect on carcass lean meat yield in New Zealand Romney sheep. Meat Sci 2017; 131: 146-151.
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