Trace metal concentrations and antioxidant activities of Tripleurospermum parviflorum and Tripleurospermum tenuifolium

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Tugce Fafal


Antioxidant activity, Trace elements,, Tripleurospermum parviflorum, Tripleurospermum tenuifolium, X-ray fluorescence


In this study, we determined the trace element contents of Tripleurospermum parviflorum and T. tenuifolium from Izmir, Turkey. The complete phenolic and flavonoid contents, as well as the antioxidant properties of methanol extracts of Tripleurospermum parviflorum and T. tenuifolium aerial parts, were also examined.  Nine minerals (Na, Ca, Mg, K, Si, Al, P, S and Ti), six trace elements (Fe, Zn, Ni, Br, Ag and Ta) were determined. The heavy metal content in percentages were determined as 9.78-11.08% and 21.63-19.94% for powdered drug and water extracts of Tripleurospermum parviflorum and T. tenuifolium, respectively. K was found the highest concentration in T. tenuifolium water extract was found to posses highest concentration of K. In addition, Na, Cl and Ca were also obtained in significant amounts in water extracts of two species. The presence of K and Ca in two Tripleurospermum species explain the traditional use of these drugs against cardiac disorders. The methanolic extract of T. parviflorum demonstrated the higher antioxidant activity than T. tenuifolium. The antioxidant activity assay findings were found to be in a strong correlation with the total phenolics and flavonoid content of the extracts.



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. Eyanet Hossain ABM. Tripleurospermum Schultz Bip. In: Davis PH, ed. Flora of Turkey and East Aegean Island, Edinburgh: Edinburgh University Press, 1975, p. 295-311
2. Cakilcioglu U, Turkoglu I. An ethnobotanical survey on medicinal plants Sivrice (Elazıg-Turkey). J Ethnopharmacol. 2010; 132: 165-175.
3. Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey, Procedia Soc Behav Sci. 2011; 19: 756-777.
4. Tetik F, Civelek S, Cakilcioglu U. Traditional uses of some medicinal plants from Malatya (Turkey). J Ethnopharmacol. 2013; 146(1): 331-346.
5. Teimouri H, Abbaszadeh S, Farzan B. An ethnobotanical study of medicinal plants with antianxiety and antidepressant effects in Shahrekord. Egypt. J Vet Sci. 2019; 50(1): 81-87.
6. Nadıroglu M, Behcet L, Cakilcioglu U. An ethnobotanical survey of medicinal plantas in Karlıova (Bingol-Turkey). Ind J Trad Know. 2019; 1(1): 76-87.
7. Harborne JB, Heywood VH, Saleh NAM. Chemosystematics of the Compositae: Flavonoid patterns in the Chrysanthemum complex of the tribe Anthemidea. Phytochemistry 1970; 9: 2011-2017.
8. Mc Laughlin JL, Chang CJ, Smith DL. Bench-Top bioassays the discovery of bioactive natural products: an update. Nat Prod Chem. 1991; 9: 383-409.
9. Yasar A, Ucuncu O, Gulec C, Inceer H, Ayaz S, Yaylı N. GC-MS analysis of chloroform extracts in flowers, stems and roots of Tripleurospermum callosum. Pharm Biol. 2005; 43(2): 108-112.
10. Souri E, Sarkhil P, Kaymanesh P, Amini M, Farsam H. Antioxidant activity of extract and a new isolated dioxapiran derivative of Tripleurospermum disciforme. Pharm Biol. 2005; 43: 620-623.
11. Servi H, Sen A, Dogan A. Chemical composition and biological activities of endemic Tripleurospermum conoclinium (Boiss. & Balansa) Heyek. Flavour Frag J. 2020; 35: 713-721.
12. Zeljkovic SC, Ayaz FA, Inceer H, Hayirlioglu-Ayaz S, Colak N. Evaluation of chemical profile and ant,oxidant activity of Tripleurospermum insularum, a new species from Turkey. Nat Prod Res. 2015; 29(3): 293-296.
13. Erdogan TF, Akkol EK, Suntar I, Gonenc TM, Kivcak B. Fatty acid compositions and antiinflamaatory activities of Tripleurospermum parviflorum (Willd.) Pobed. and Tripleurospermum tenuifolium (Kit.). Rec Nat Prod. 2015; 9: 394-403.
14. Minaiyan M, Ghassemi-Dehkordi N, Mohammadzadeh B. Anti-ulser effect of Tripleurospermum disciforme (CA Mey) Shultz Bip on pylorus ligated (Shay) rats. Res Pharm Sci. 2007; 1: 15-21.
15. Amin G, Dehmoobed-Sharifabadi A, Surmaghi MS, Yasa N, Aynechi Y, Emami M, Shidfar MR, Amin M, Moghadami M, Kordbacheh P, Zeyni F. Screening of Iranian plants for antifungal activity. Daru J Pharm Sci. 2002; 10: 38-48.
16. Mark PE, Michael JB, Jianwei WH, Christopher DG. Plants as a natural source of concentrated mineral nutritional supplements. Food Chem. 2000; 71(2): 181-188.
17. Schumacher M, Bosque MA, Domingo JL, Corbella J. Dietary intake of lead and cadmium from foods in Tarragona Province, Spain. Environ Contam Tox. 1991; 46(2): 320-328.
18. Babu K, Rajkishore VB, Margesan T, Narayanan J, Margesan T. Analysis of heavy metals and inorganic element content in Stereospermum colais leaves. Int J Curr Pharm Res. 2013; 2(3): 63-66.
19. Fukumoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem. 2000; 48(8): 3597-3604.
20. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Adv Free Radic Biol Med. 1999; 26(9-10): 1231-1237.
21. Apak R, Guclu K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem. 2004; 52(26): 7970-7981.
22. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965; 6: 144-158.
23. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10: 178-182.
24. Pandey KB, Rızvı SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Long 2009; 2(5): 270-278.
25. Jr DW, Martin PA, Mayers VW, Rodwell DK, Granner, Harper’s Review of Biochemistry. 20th ed. Lange Medical Publications, California, 1985, p. 651.
26. Curran ME. Potassium targets, ion channels and human disease:phenotypes to drug. Cur Opin Biotechnol. 1998; 9(6): 565-572.
27. Ekinci N, Ekinci R, Polat R, Budak G. Analysis of trace elements in medicinal plants with enegy dispersive X-ray fluorescence. J Radional Nucl Chem. 2004; 260: 127-131.
28. Yagi S, Rahman AE, ELhassan GOM, Abdelhafeez MA. Elemental analysis of ten Sudanese medicinal plants using X-ray fluorescence. J Appl Sci Res. 2013; 1(1): 49-53.
29. Loeper J, Fragny M. The physiological role of the silicon and its antiatheromatous action, Biochemistry of Silicon and Releated Problems Nobel Foundation Symposia 1978; 40: 281.