Does persimmon leaf have a protective effect against oxidative damage caused by chromium in Saccharomyces cerevisiae?

Main Article Content

Ozlem Gok
Seda Beyaz
Fazilet Erman
Abdullah Aslan

Keywords

Cell development, oxidative damage, persimmon leaf, Saccharomyces cerevisiae, SDS-PAGE

Abstract

In this study, 4 groups were formed. Groups; (i) Control group; (ii) Persimmon Leaf Group (10%); (iii) Chromium (K2Cr2O7) Group (10 mM); (iv) Persimmon Leaf (10%) + Chromium (K2Cr2O7) (10 mM) Group. Saccharomyces cerevisiae (S. cerevisiae) cultures were grown at 30 °C for 1 hour, 3 hours, 5 hours and 24 hours. Cell growth, lipid peroxidation, MDA (malondialdehyde) analyzes, glutathione (GSH) levels and catalase activities were determined by spectrophotometer. Total protein changes were detected by SDS-PAGE electrophoresis and calculated by the Bradford method. According to the results obtained; cell growth (1, 3, 5 and 24 hours), total protein synthesis (24 hours), GSH levels (24 hours) and catalase activities (24 hours) in the Persimmon Leaf + Chromium group compared to the Chromium group while increasing, MDA level (24 hours) decreased. These results show that persimmon leaf reduces oxidative damage, enhances cell growth and it has a protective effect to promote protein synthesis in S. cerevisiae culture

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