Evaluation of Taste Related Genes (CD 36, SHH, PLCß2) Expression in Saliva

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Elif Esra Ozturk https://orcid.org/0000-0003-1097-6325
Derya Dikmen https://orcid.org/0000-0003-2099-2863


unstimulated saliva, lemon-juice stimulated saliva, gene expression, taste


Due to its non-invasiveness accessibility and high availability in diagnostic and prognostic evaluation, the use of saliva has gradually gained popularity in recent years. This study's objective was to examine the expression level of taste-related genes in saliva (SHH, CD 36, PLCß2). The study was carried out in two stages. In the first stage, both lemon-juice stimulated and unstimulated saliva was gathered from 10 participants (6 males and 4 females) to examine the effect of stimulation with citric acid on expression level. Forty-six subjects (22 males, 24 females) participated in the second stage, and unstimulated saliva was collected due to the quality and yield of the mRNA obtained to examine the expression level of taste-related genes (SHH, CD 36, PLCß2). There was no difference between SHH expression levels in saliva stimulated with lemon juice and unstimulated saliva. The results showed that, SHH, CD 36, PLCß2 expression was higher in unstimulated saliva (p>0.05). The study demonstrated that unstimulated saliva is more suitable for determining taste-related gene expression levels.



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1. Humphrey SP, Williamson RT. A review of saliva: normal composition, flow, and function. The Journal of prosthetic dentistry. 2001; 85:162-9.
2. Santosh TS, Parmar R, Anand H, Srikanth K, Saritha M. A review of salivary diagnostics and its potential implication in detection of Covid-19. Cureus. 2020; 12.
3. Muñoz-González C, Feron G, Canon F. Main effects of human saliva on flavour perception and the potential contribution to food consumption. Proceedings of the Nutrition Society. 2018; 77:423-31.
4. Canon F, Neiers F, Guichard E. Saliva and flavor perception: perspectives. Journal of agricultural and food chemistry. 2018; 66:7873-9.
5. Soell M, Feki A, Hannig M, Sano H, Pinget M, Selimović D. CHROMOGRANIN A DETECTION IN SALIVA OF ΤΥΡΕ 2 DIABETES PATIENTS. Bosnian journal of basic medical sciences. 2010; 10:2.
6. Takai N, Yamaguchi M, Aragaki T, Eto K, Uchihashi K, Nishikawa Y. Effect of psychological stress on the salivary cortisol and amylase levels in healthy young adults. Archives of oral biology. 2004; 49:963-8.
7. Poduval J. Saliva in diagnostics-a useful screening tool. The Journal of Medical Research. 2016; 2:01-2.
8. Qvarnstrom M, Janket SJ, Jones JA, Jethwani K, Nuutinen P, Garcia RI, et al. Association of salivary lysozyme and C‐reactive protein with metabolic syndrome. Journal of clinical periodontology. 2010; 37:805-11.
9. Hezel M, Weitzberg E. The oral microbiome and nitric oxide homoeostasis. Oral diseases. 2015; 21:7-16.
10. Feng Y, Licandro H, Martin C, Septier C, Zhao M, Neyraud E, et al. The associations between biochemical and microbiological variables and taste differ in whole saliva and in the film lining the tongue. BioMed research international. 2018; 2018.
11. Henson BS, Wong DT. Collection, storage, and processing of saliva samples for downstream molecular applications. Oral Biology: Springer; 2010. p. 21-30.
12. Archer NS, Liu D, Shaw J, Hannan G, Duesing K, Keast R. A comparison of collection techniques for gene expression analysis of human oral taste tissue. PloS one. 2016; 11:e0152157.
13. Jasim H, Carlsson A, Hedenberg-Magnusson B, Ghafouri B, Ernberg M. Saliva as a medium to detect and measure biomarkers related to pain. Scientific reports. 2018; 8:1-9.
14. Priya KY, Prathibha KM. Methods of collection of saliva-a review. International Journal of Oral Health Dentistry. 2017; 3:149-53.
15. Penniston KL, Nakada SY, Holmes RP, Assimos DG. Quantitative assessment of citric acid in lemon juice, lime juice, and commercially-available fruit juice products. Journal of Endourology. 2008; 22:567-70.
16. Mohamed R, Campbell J-L, Cooper-White J, Dimeski G, Punyadeera C. The impact of saliva collection and processing methods on CRP, IgE, and Myoglobin immunoassays. Clinical and translational medicine. 2012; 1:19.
17. Pandey M, Reddy V, Wanjari PV. Comparative evaluation of citric acid and TENS as means for salivary stimulation in adults: An Invivo study. Journal of Indian Academy of Oral Medicine and Radiology. 2019; 31:36.
18. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods. 2001; 25:402-8.