Prevalence of, and Genetic Relationship Between, Coliforms in Food, Foodhandlers and Contact Surfaces Factors Affecting Food Hygiene

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Şebnem Pamuk
Metin Erdoğan


Coliforms, Contamination, E. coli, Food, Genotyping


Coliforms have been used as indicator organisms in examinations of food for fecal contamination and for the identification of unsanitary conditions in food outlets. In the present study we determine the prevalence of coliforms isolated from foods, kitchen utensils and the hands of those involved in the preparation of food.  The study is based on, samples collected from 100 food items, 45 foodhandlers’ hands, 27 cutting boards and 40 knife handle surfaces in six different canteens on a university campus during the 2017- 2018 academic year. The presence of coliforms was confirmed with biochemical tests and the PCR amplification of 16s rRNA. Gradient PCR was used subsequently for isolation followed by a sequence analysis to determine any association. In all, 14 Escherichia coli, 32 Klebsiella pneumoniae ve altı Citrobacter spp. izolatlar, bir gradyan PCR ve sekans analizinde gıda işçisinin ellerinden, yiyeceklerinden ve yüzeylerinden tanımlandı. Ayrıca ellerde ve kesme tahtalarında, sosis, ızgara tavuk, sotelenmiş tavuk, Urfa kebabı ve tavuk dönerde E. coli izolatları;  Klebsiella pneumoniae eller, ızgara tavuk, bıçak sapları, köfte ve kesme tahtalarından izole edildi; ve Citrobacter spp. el, sosis, kesme tahtaları ve ızgara tavuklardan elde edildi.


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1. Oranusi S, Omagbemi F, Eni AO. Microbiological safety evaluation of snacks sold in fast food shops in Ota, Ogun state, Nigeria. Int J Agri Food Sci 2011; 1(4): 75-79.
2. Kotzekidou P. Food Hygiene and Toxicology in Ready-to-Eat Foods. 5th (Ed) Academic
Press, UK., 2016.
3. Domínguez C, Gómez I, Zumalacárregui J. Prevalence of Salmonella and Campylobacter in retail chicken meat in Spain. Int J Food Microbiol 2002; 72: 165-168.
4. Little CL, Gillespie IA: Prepared salads and public health. J App Microbiol 2008; 105: 1729-1743.
5. Meleko A, Henok A, Tefera W, Lamaro, T. Assessment of the sanitary conditions of catering establishments and food safety knowledge and practices of food handlers in Addis Ababa University Students’ Cafeteria. Sci J Public Health 2015; 3(5): 733-743.
6. Elikwu CJ, Shobowale EO, Nwadike VU, Tayo B, Okangba CC, Shonekan OA, Omeonu AC, Faluyi B, Ile P, Adelodun A, Popoola A, Mubele M. Antimicrobial susceptibility patterns of Enterobacteriaceae isolated from stool samples at a semi-urban teaching hospital. Am J Biomed Life Sci 2015; 3(6): 127-130.
7. Hold T. Foodborne Infections Bacteria. In: JG, ME Morris (Ed): Foodborne Infections and Intoxications. 67-91. Academic Press, UK, London. 2016.
8. Singh A, Fleurat M, Kwon KT, Whiteman, P. Pediatric emergency medicine practice acute gastroenteritis-an update. Pedi Emerg Med Prac 2010; 7(7): 1-20.
9. Barza M. Efficacy and tolerability of ClO2-generating gloves. Clin Infec Dis 38; 857-863.
10. Oranusi S, Galadima M, Umoh VJ, Nwanze PI. Food safety evaluation in boarding schools in Zaria, Nigeria, using the HACCP system. Sci Res Essay 2007; 2(10): 426-433.
11. Shojaei H, Shooshtaripoor J, Amiri M. Efficacy of simple hand-washing in reduction of microbial hand contamination of Iranian food handlers. Food Res Int 2005; 39: 525-529.
12. Todd ECD, Greig JD, Bartleson CA, Michaels BS. Outbreaks where food workers have been implicated in the spread of foodborne disease. Sources of contamination and pathogen excretion from infected persons. J Food Protect 2008; 71(12): 2582-2595.
13. Uyttendaele M, Jaykus LA, Amoah P, Chiodini A, Cunliffe D, Jacxsens L, Holvoet K, Korsten L, Lau M, McClure P, Medema G, Sampers I: Microbial hazards in irrigation water: standards, norms, and testing to manage use of water in fresh produce primary production. Comp Rev Food Sci Food Safety 2015; 14: 336-356.
14. Blood RM, Curtis GDW. Media for ‘total’ enterobacteriaceae, coliforms and Escherichia coli. Int J Food Microbiol, 1995; 26: 93-115.
15. Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol, 1987; 4: 406-425.
16. Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 1985; 39: 783-791.
17. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. 2004. Available: (Accessed October 26, 2019).
18. Tamura K, Dudley J, Nei M, Kumar S: Molecular evolutionary genetics analysis (MEGA4) software version 4.0. 2007. Available: (Accessed October 26, 2019).
19. Hon-Ming L, Remais J, Fung MC, Xu L, Sun SSM. Food supply and food safety issues in China. Lancet 2013; 381.
20. Kusumaningrum HD, Van Putten MM, Rombouts FM, Beumer RR. International association for food protection effects of antibacterial dishwashing liquid on foodborne pathogens and competitive microorganisms in kitchen sponges. J Food Protec 2002a; 65 (1): 61-65.
21. World Health Organization: Diarrhoea: why children are still dying and what can be done, Geneva. 2009. Available: (Accessed November 17, 2019).
22. Omemua AM, Aderoju ST. Food safety knowledge and practices of street food vendors in the city of Abeokuta, Nigeria. Food Control 2008; 19: 396-402.
23. Rane, S. Street vended food in developing world: Hazard analyses. Indian J of Microbiol 2011; 51(1): 100-106.
24. Crump JA, Stephen PL, Mintz ED. The global burden of typhoid fever. Bull World Health Org, 2004; 82(5): 346-352.
25. Havelaar AH, Kirk MD, Torgerson PR, Gibb HJ, Hald T, Lake RJ, Praet N, Bellinger DC, de Silva NR, Gargouri N, Speybroeck N, Cawthorne A, Mathers C, Stein C, Angulo FJ. World Health Organization global estimates and regional comparisons of the burden of foodborne disease in 2010. PLOS Medicine 2015; 1-23.
26. Khairuzzaman MD, Chowdhury FM, Zaman S, Al Mamun, Arafat, Latiful Bari, MD. Food safety challenges towards safe, healthy, and nutritious street foods in Bangladesh. Int J Food Sci 2014; 1-9.
27. Den Aantrekker ED, Boom RM, Zwietering MH, Van Schothorst, M. Quantifying recontamination through factory environments-A review. Int J Food Microbiol 2003; 80(2): 117-130.
28. Kusumaningrum HD, Van Putten MM, Rombouts FM, Beumer RR. Effects of antibacterial dishwashing liquid on foodborne pathogens and competitive microorganisms in kitchen sponges. J Food Protect 2002b; 65: 61-65.
29. Kusumaningrum HD, Riboldi G, Hazeleger WC, Beumer RR. Survival of foodborne pathogens on stainless steel surfaces and cross-contamination to foods. Int J Food Microbiol 2003; 85: 227-236.
30. Pamuk Ş, Erdoğan M, Yıldırım Y, Hizlisoy H, Al S, Sepin Ö. Evaluation of hand hygiene situation of foodhandlers and microbiological quality of retailed some foods in university campus canteen. Kocatepe Vet J 2018; 11(4): 363-373.
31. Lues JFR, Van Tonder I. The occurrence of indicator bacteria on hands and aprons of food handlers in the delicatessen sections of a retail group. Food Control 2007; 18: 326-32.
32. Lee HK, Hali HA, Thong KL, Chai LC. Assessment of food safety knowledge, attitude, self-reported practices, and microbiological hand hygiene of food handlers. Int J Environ Res Public Health 2017; 14: 55.
33. Ayuningtya NV, Fitria L: Escherichia coli contamination in elementary school snacks: A cross-sectional study. Indian J Public Health Res Develop 2020; 11: 4.
34. Gil AI, Lanata CF, Hartinger SM, Mäusezahl D, Padilla B, Theresa J, Lozada OM, Pineda I, Verastegui H: Fecal contamination of food, water, hands, and kitchen utensils at the household level in rural areas of Peru. Adv Sci 2014; 76: 102-107.
35. Chavatte N, Bare´ J, Lambrecht E, Van Damme I, Vaerewijck M, Sabbe K, Houf K: Co-occurrence of free-living protozoa and foodborne pathogens on dishcloths: implications for food safety. Int J Food Microbiol 2014; 191: 89-96.

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