Influences of Pistacia terebinthus L. and Rhus coriaria L. fruits extracts on the levels of some biochemical parameters in liver tissue of Experimental Breast Cancer rats

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Ayse Dilek Ozsahin
Figen Erdem Erisir
Zeynep Tuzcu


Breast cancer, Rhus coriaria, Pistacia terebinthus, Liver tissue, Lipid peroxidation, Antioxidant enzyme, Fatty acid


In this study, against to DMBA-induced breast cancer healing effects of Pistacia terebinthus L. subsp. Palaestina (terebinth) and Rhus coriaria L. (sumac) were examined as biochemical in the female Sprague-Dawley rats. 66 rats were used in this study. Rats were divided into 6 groups as Control, DMBA, PT, RC, PT+DMBA and RC+DMBA. DMBA was administered by gavaged, a single dose of 80 mg/kg according to body weight, to 8-week-old rats. Aqueous extracts of terebinth and sumac were given orally 3 days a week to rats in antioxidant groups.

Malondialdehyde, glutathione, vitamin, cholesterol, antioxidant enzyme and fatty acid levels, which are signs of lipid peroxidation, were measured in liver tissue.  At the end of the study in cancerous rats, malondialdehyde increased compared to the control group. Glutathione decreased in the other tissues and all of the DMBA-induced cancerous rats groups. Lipophilic vitamins and cholesterol levels were analyzed by HPLC. The levels were different in DMBA and antioxidant groups of tissues.  In cancerous rats, liver cholesterol levels decreased.

The results of the present study showed that the herb suspensions exerted anti-cancer effects and consequently may alleviative liver damage caused by DMBA-induced breast cancer.


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1. GLOBOCAN. Global Cancer Observatory, Inter Agen Res Cancer. 2018; (
2. İçen MS, Karakuş F, Tosun E, Yılmaz K. Antiproliferative Potential of Pistacia terebinthus L. on Breast Cancer Cell Lines. Conference: Gazi Pharma Symposium Series. 2015.
3. Mansour HA, Newairy AA, Yousef MI, Sheweita SA. Biochemical Study on The Effects of Some Egyptian Herbs in Alloxan-Induce. Diabetic Rats. Toxicol 2002; 170: 221-228.
4. El Hasasna H, Athamneh K, Al Samri H, Karuvantevida N, Al Dhaheri Y, Hisaindee S, Ramadan G, Al Tamimi N, AbuQamar S, Eid A, Iratni R. Rhus coriaria induces senescence and autophagic cell death in breast cancer cells through a mechanism involving p38 and ERK1/2 activation. Sci Rep 205; 5: 13013.
5. Kavak D, Altıok E, Bayraktar O, Ülkü S. Pistacia terebinthus extract: As a potential antioxidant, antimicrobial and possible β- glucuronidase inhibitor. J Mol Catalysis B: Enzym 2010; 64: 167-171.
6. Topçu G, Ay M, Bilici A, Sarıkürkcü C, Öztürk M, Ulubelen A. A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chem 2007;103: 816-822.
7. Mundhe NA, Kumar P, Ahmed S, Jamdade V, Mundhe S, Lahkar M. Nordihydroguaiaretic acid ameliorates cisplatin induced nephrotoxicity and potentiates its anti-tumor activity in DMBA induced breast cancer in female Sprague–Dawley rats. Inter Immunopharmacol 205; 28: 634–642.
8. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1972; 95: 351-358.
9. Elman GI. Tissue Sulfhydryl Groups. Arch Bioc 1959; 70-77.
10. Hara A, Radin NS Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem 1978; 90: 420–426.
11. Christie WW. Gas chromatography and lipids. The Oil 1999.
12. Sanchez-Machado DI, Lopez-Hernandez J, Paseiro-Losada P, Lopez-Cervantes J. An HPLC method for the quantification of sterols in edible sea weeds. Biomed Chromato 2004; 18: 183-90.
13. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. The J Biol Chem 1951; 193(1): 265-275.
14. Panchenko LF, Brusov OS, Gerasimov AM, Loktaeva TD. Intramitochondrial localization and release of rat liver superoxide dismutase. Febs Lett 1975; 55: 84–87.
15. Bell JG, Cowey CB, Adron JW, Shanks AM. Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout (Salmo gairdneri). British J Nutri 1985; 53: 149–157.
16. Aebi H. Catalase In vitro. Methods in Enzymol 1984; 105: 121-126.
17. Zargham H, Zargham R. Tannin extracted from Sumac inhibits vascular smooth muscle cell migration. MJM 2008; 11:119–123.
18. Anwer T, Sharma M, Khan G, Iqbal M, Ali MS, Alam MS, Safhi MM, Gupta N. Rhus coriaria ameliorates insulin resistance in non-insulin-dependent diabetes mellitus (NIDDM) rats. Acta Poloniae Pharma- Drug Res 2013; 70: 861-867.
19. Shabbir A. Rhus coriaria linn, a plant of medicinal, nutritional and industrial importance: A review. J Anim Plant Sci 2012; 22: 505-512.
20. Panico A, Cardile V, Santagati NA, Messina R. Antioxidant and protective effects of Sumac Leaves on Chondrocytes. J Med Plants Res 2009; 3: 855–861.
21. Chakraborty A, Ferk F, Simić T, Brantne A, Dušinská M, Kundi M, Hoelzl C, Nersesyan A, Knasmüller S. DNA-protective effects of sumach (Rhus coriaria L.), a common spice: results of human and animal studies. Mutation Res/Funda Molecular Mech Mutag 2009; 661: 10-17.
22. Pourahmad J, Eskandari MR, Shakibaei R, Kamalinejad M. A search for hepatoprotective activity of aqueous extract of Rhus coriaria L. against oxidative stress cytotoxicity. Food Chem Toxicol 2010; 48: 854-858.
23. Özdemir I, Selamoglu Z, Ates B, Gok Y, Yilmaz I. Modulation of DMBA-induced biochemical changes by organoselenium compounds in blood of rats. Indian J Biochem Biophys 2007; 44: 257-259.
24. Zarrini AS, Moslemi D, Pasian H, Vessal M, Mosapour a, Kelagari ZS. The status of antioxidants, malondialdehyde and some trace elements in serum of patients with breast cancer. Caspian J Intern Med 2016; 7(1): 31-36.
25. Coughlin SS. Oxidative Stress, Antioxidants, Physical Activity, and the Prevention of Breast Cancer Initiation and Progression. J Environ Health Sci 2018; 4: 55-57.
26. Jezierska-Drutel A, Rosenzweig SA, Neumann CA. Role of oxidative stress and the microenvironment in breast cancer development and progression. Adv Cancer Res 2013; 119: 107–125.
27. Beutler E. Nutritional and metabolic aspects of glutathione. Ann Rev Nutr 1989; 9: 287-302.
28. Sies H. Glutathione and its role in cellular functions. Free Rad Biol Med 1990; 27: 916–921.
29. Schumacker PT. Reactive oxygen species in cancer: a dance with the devil. Cancer Cell 2015; 27: 156–157.
30. Ramírez-Expósito MJ, Urbano-Polo N, Dueñas B, Navarro-Cecilia J, Ramírez-Tortosa C, Martín-Salvago MD, Martínez-Martos JM. Redox status in the sentinel lymph node of women with breast cancer. Upsala J Med Sci 2017; 122 207-216.
31. Martins JM, Riotto M, Abreu MC, Lanca MJ, Viegas-Crespo AM, Aimeida JA. Cholesterol-Lowering Effects of Dietary Blue Lupin (Lupinus angustifolius L.) in Intact and Ileorectal Anastomosed Pigs. J Lipid Res 2005; 46:1539-1547.
32. De La Puente-Yagüe M, Cuadrado-Cenzual MA, Ciudad-Cabañas MJ, Hernández-Cabria M, Collado-Yurrita L. Vitamin D: and its role in breast cancer. The Kaohsiung J Med Sci. 2018; 34: 423-427.
33. Abbas S, Chang‐Claude J, Linseisen J. Plasma 25‐hydroxyvitamin D and premenopausal breast cancer risk in a German case‐control study. Inter J Cancer. 2009; 124: 250-255.
34. Schwenke DC. Does lack of tocopherols and tocotrienols put women at increased risk of breast cancer. J Nutr Biochem 2002; 13: 2–20.
35. Ge Y, Chen Z, Kang ZB, Cluette-Brown J, Laposata M, Kang JX. Effects of adenoviral gene transfer of C. elegans n-3 fatty acid desaturase on the lipid profile and growth of human breast cancer cells. Anticancer Res. 2002; 22: 537-544.
36. Hilvo M, Denkert C, Lehtinen L, Muller B, Brockmoller S, Seppanen-Laakso T, Budczies J, Bucher E, Yetukuri L, Castillo S, Berg E, Nygren H, Sysi-Aho M, Griffin JL, Fiehn O, Loibl S, Richter-Ehrenstein C, Radke C, Hyötyläinen T, Kallioniemi O, Iljin K, Oresic M. Novel theranostic opportunities offered by characterization of altered membrane lipid metabolism in breast cancer progression. Cancer Res 2011; 71: 3236–3245.
37. Qiu Y, Zhou B, Su M, Baxter S, Zheng X, Zhao X, Yen Y, Jia W. Mass spectrometry-based quantitative metabolomics revealed a distinct lipid profile in breast cancer patients. Inter J Mol Sci 2013; 14: 8047-8061.
38. Hasija K, Bagga HK. Alterations of serum cholesterol and serum lipoprotein in breast cancer of women. Indian J Clin Biochem 2005; 20: 61-66.
39. Shah FD, Shukla SN, Shah PM, Patel HR, Patel PS. Significance of alterations in plasma lipid profile levels in breast cancer. Integrative Cancer Thera 2008; 7: 33-41.

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