Proteins for protein synthesis: Quantity and timing
Keywords:
protein , protein synthesis, Protein intakeAbstract
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References
1. FAO/WHO/UNU. Protein and amino acid requirements in human nutrition. WHO Press; 2007. (Technical Report Series 935).
2. Kurpad AV, Thomas T. Methods to assess amino acid requirements in humans. Curr Opin Clin Nutr Metab Care. 2011 Sep;14(5):434-9.
3. Moore DR, Robinson MJ, Fry JL, et al. Ingested protein dose-response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr. 2008;89(1):161-8.
4. Nair KS, Jaleel A, Asmann YW, Short KR, Raghavakaimal S. Proteomic research: potential opportunities for clinical and physiological investigators. Am J Physiol Endocrinol Metab. 2004;286(5):E863-74.
5. Chinkes DL, Rosenblatt J, Wolfe RR. Assessment of the mathematical issues involved in measuring the fractional synthesis rate of protein using the flooding dose technique. Clin Sci (Lond). 1993 Feb;84(2):177-83.
6. Halliday D, McKeran RO. Measurement of muscle protein synthetic rate from serial muscle biopsies and total body protein turnover in man by continuous intravenous infusion of L-(15N)lysine. Clin Sci Mol Med. 1975;49(6):581-90.
7. Esmarck B, Andersen JL, Olsen S, Richter EA, Mizuno M, Kjaer M. Timing of postexercise protein intake is important for muscle hypertrophy with resistance training in elderly humans. J Physiol. 2001;535(Pt 1):301-11. doi: 10.1111/j.1469-7793.2001.00301.x.
8. Rasmussen BB, Tipton KD, Miller SL, Wolf SE, Wolfe RR. An oral essential amino acid-carbohydrate supplement enhances muscle protein anabolism after resistance exercise. J Appl Physiol (1985). 2000;88(2):386-92. doi: 10.1152/jappl.2000.88.2.386.
9. Aragon AA, Schoenfeld BJ. Nutrient timing revisited: is there a post-exercise anabolic window? J Int Soc Sports Nutr. 2013;10:5.
10. Karlsson HK, Nilsson PA, Nilsson J, Chibalin AV, Zierath JR, Blomstrand E. Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise. Am J Physiol Endocrinol Metab. 2004;287(1):E1-7.
11. Dreyer HC, Drummond MJ, Pennings B, et al. Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle. Am J Physiol Endocrinol Metab. 2008;294(2):E392-400.
12. Dreyer HC, Fujita S, Cadenas JG, Chinkes DL, Volpi E, Rasmussen BB. Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle. J Physiol. 2006;576(2):613-24.
13. Tipton KD, Phillips SM. Dietary protein for muscle hypertrophy. Limits Hum Endur. 2013;76:73-84.
14. Mazzulla M, Abou Sawan S, Williamson E, et al. Protein intake to maximize whole-body anabolism during postexercise recovery in resistance-trained men with high habitual intakes is severalfold greater than the current recommended dietary allowance. J Nutr. 2020;150(3):505-11.
15. Hudson JL, Wang Y, Bergia RE 3rd, Campbell WW. Protein intake greater than the RDA differentially influences whole-body lean mass responses to purposeful catabolic and anabolic stressors: a systematic review and meta-analysis. Adv Nutr. 2020;11(3):548-58.
16. Atherton C, McNaughton LR, Close GL, Sparks A. Post-exercise provision of 40 g of protein during whole body resistance training further augments strength adaptations in elderly males. Res Sports Med. 2020;28(4):469-83.
17. Tagawa R, Watanabe D, Ito K, et al. Synergistic effect of increased total protein intake and strength training on muscle strength: a dose-response meta-analysis of randomized controlled trials. Sports Med Open. 2022;8(1):110. doi: 10.1186/s40798-022-00508-w.
18. Poortmans JR, Dellalieux O. Do regular high-protein diets have potential health risks on kidney function in athletes? Int J Sport Nutr Exerc Metab. 2000;10(1):28-38. doi: 10.1123/ijsnem.10.1.28.
19. Antonio J, Ellerbroek A, Silver T, et al. A high-protein diet has no harmful effects: a one-year crossover study in resistance-trained males. J Nutr Metab. 2016;2016:9104792. doi: 10.1155/2016/9104792.
20. Cheng Y, Zheng G, Song Z, et al. Association between dietary protein intake and risk of chronic kidney disease: a systematic review and meta-analysis. Front Nutr. 2024;11:1408424. doi: 10.3389/fnut.2024.1408424.
21. Antonio J, Ellerbroek A, Carson C. The effects of a high-protein diet on bone mineral density in exercise-trained women: a one-year investigation. J Funct Morphol Kinesiol. 2018;3(4):62. doi: 10.3390/jfmk3040062.
22. Bagheri R, Karimi Z, Mousavi Z, et al. High-protein diets during either resistance or concurrent training have no detrimental effect on bone parameters in resistance-trained males. Nutrients. 2024;16(2):325. doi: 10.3390/nu16020325.
23. Antonio J, Evans C, Ferrando AA, et al. Common questions and misconceptions about protein supplementation: what does the scientific evidence really show? J Int Soc Sports Nutr. 2024;21(1):2341903.
24. Areta JL, Burke LM, Ross ML, et al. Timing and distribution of protein ingestion during prolonged recovery from resistance exercise alters myofibrillar protein synthesis. J Physiol. 2013;591(9):2319-33. doi: 10.1113/jphysiol.2012.244897.
25. Moore DR. Maximizing post-exercise anabolism: the case for relative protein intakes. Front Nutr. 2019;6:147. doi: 10.3389/fnut.2019.00147.
26. Witard OC, Mettler S. The anabolic response to protein ingestion during recovery from exercise has no upper limit in magnitude and duration in vivo in humans: a commentary. Int J Sport Nutr Exerc Metab. 2024:1-3. doi: 10.1123/ijsnem.2024-0041.
27. Mallinson JE, Wardle SL, O'Leary TJ, et al. Protein dose requirements to maximize skeletal muscle protein synthesis after repeated bouts of resistance exercise in young trained women. Scand J Med Sci Sports. 2023;33(12):2470-81. doi: 10.1111/sms.14506.
28. Trommelen J, van Lieshout GAA, Nyakayiru J, et al. The anabolic response to protein ingestion during recovery from exercise has no upper limit in magnitude and duration in vivo in humans. Cell Rep Med. 2023;4(12).
29. Yasuda J, Tomita T, Arimitsu T, Fujita S. Evenly distributed protein intake over 3 meals augments resistance exercise-induced muscle hypertrophy in healthy young men. J Nutr. 2020;150(7):1845-51. doi: 10.1093/jn/nxaa101.
30. Biolo G, Maggi SP, Williams BD, Tipton KD, Wolfe RR. Increased rates of muscle protein turnover and amino acid transport after resistance exercise in humans. Am J Physiol Endocrinol Metab. 1995;268(3):E514-20.
31. Trommelen J, van Lieshout GAA, Pabla P, et al. Pre-sleep protein ingestion increases mitochondrial protein synthesis rates during overnight recovery from endurance exercise: a randomized controlled trial. Sports Med. 2023;53(9):1445-55.
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