In vitro antibacterial efficiency of some herbal extracts and theirs blends against subclinical mastitis pathogens (S. aureus and E. coli)

Main Article Content

Hanifi Aydın

Keywords

O.sanctum, O.onites, subclinicalmastitis, dairy cows, in vitro

Abstract

Study Objectives: The current research will form the basis for the production of new herbal mastitis drugs as an alternative to antibiotics. In this context, the aim of the study was to evaluate the in vitro antibacterial activity of O. onites and O. sanctum essential oil, solutions prepared in fixed oils of N. sativa and O. europaea, against S. aureus and E. coli bacteria which are the main agents of mastitis. Methods: Microorganism isolation and antibiotic susceptibility analyzes of milk samples taken from 43 subclinical mastitis dairy cows were performed. The antibacterial properties of the solutions were analyzed by a disk diffusion method. Results: In the bacterial isolation results, S.aureus was determined 97.7% and E.coli 34.9% positive of cows with mastitis. Occur that extract of O. onites showed maximum inhibition against S. aureus (23 mm) and E. coli (15 mm). At the same time, E.coli, which is already known to be multi-resistant to drugs, was susceptible only to the extract from O.onites. When the mean ± SD zone areas for the susceptible solutions (C, C1, C2, O, S, O+S, and Z2) and the control group were compared, determined that there was no statistically significant difference for S.aureus and E.coli (p>0.05). Conclusion: It was determined that the effectiveness of olive oil as a solvent against S. aureus was not sufficient, therefore, a 50% solution (15mm zone diameter) with N. sativa fixed oil was more effective.

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References

1. Alonso MM, Salazar JCL, Robles SO, Guerrero IC, García FL, Marrero JG. In vitro antimicrobial activity of mexican plants on bovine mastitis bacteria: preliminary studies. Bioscience Journal 2020; 36(1): 183-190.
2. Harjanti DW, & Sambodho P. Effects of mastitis on milk production and composition in dairy cows. In IOP Conference Series: Earth and Environmental Science IOP Publishing 2020 September; 518(1): 012032.
3. Amber R, Adnan M, Tariq A, Khan SN, Mussarat S, Hashem A, AbdAllah EF. Antibacterial activity of selected medicinal plants of northwest Pakistan traditionally used against mastitis in livestock. Saudi Journal of Biological Sciences 2018; 25(1): 154-161.
4. Klaas IC, Zadoks RN. An update on environmental mastitis: challenging perceptions. Transboundary and Emerging Diseases 2018; 65: 166-185.
5. Suleiman TS, Karimuribo ED, & Mdegela RH. Prevalence of bovine subclinical mastitis and antibiotic susceptibility patterns of major mastitis pathogens isolated in Unguja island of Zanzibar, Tanzania. Tropical Animal Health and Production 2018; 50(2): 259-266.
6. Lopes TS. Estudo sobre a ação de óleos essenciais no controle de microrganismos causadores de mastite bovina. Universidade de Caxias do Sul, Master Thesis 2020; pp 158
7. Radzikowski D, Kalińska A, Ostaszewska U, & Gołębiewski M. Alternative solutions to antibiotics in mastitis treatment for dairy cows-a review. Animal Science Papers & Reports 2020; 38(2): 117-133
8. Tepeli SÖ. Origanum onites ve Ocimum basilicum’un blaCTX-M pozitif enterobacteriaceae üzerine antimikrobiyal etkisi. Journal of Advanced Research in Natural and Applied Sciences 2020; 6(2): 206-216.
9. Gucwa K, Milewski S, Dymerski T, & Szweda P. Investigation of the antifungal activity and mode of action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum and Eugenia caryophyllus essential oils. Molecules 2018; 23(5): 1116, 1-18.
10. Dinçoğlu AH. Mercanköşk (Origanum Onites L.) bitkisinin bazı gıda patojenleri üzerine antibakteriyel etkinliğinin saptanması. Mehmet Akif Ersoy Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 2019; 7(2): 139-146.
11. Napoli E, Siracusa L, & Ruberto G. New tricks for old guys recent developments in the chemistry, biochemistry, applications and exploitation of selected species from the Lamiaceae family. Chemistry & Biodiversity 2020; 17(3): e1900677.
12. Bano N, Ahmed A, Tanveer M, Khan GM, & Ansari MT. Pharmacological evaluation of Ocimum sanctum. Journal of Bioequivalence and Bioavailability 2017; 9(3): 387-492.
13. Shafi TA, Bansal BK, & Gupta DK. In vitro antibacterial activity and minimum inhibitory concentration of Ocimum sanctum leaves against common bovine mastitis pathogens. J Dairy Vet Anim Res 2018; 7(6): 322-324.
14. Sharma D. Studies on therapeutic potential of Ocimum sanctum on subclinical mastitis in goats (Capra hircus). Doctoral dissertation, Rajasthan University of Veterinary and Animal Sciences Bikaner 2018; 1-113.
15. Rakhshandeh H, & Vahdati-Mashhadian N. In vitro and in vivo study of the antibacterial effects of Nigella sativa methanol extract in dairy cow mastitis. Avicenna Journal of Phytomedicine 2011; 1(1): 29-35.
16. Uras SS. Nigella sativa L. (Ranunculaceae) bitkisi üzerinde farmakognozik araştırmalar Mersin Üniversitesi, Sağlık Bilimleri Enstitüsü, Farmakognozi Anabilim Dalı Yl 2009; p.135.
17. Salem AY, El-Awady HG, El-Dein MAT & Eisa DA. Effect of supplementation of aromatic plants oils on immunity, udder health and milk production of Friesian cows. Slov. Vet. Res. 2019; 58(22): 523-530.
18. Cicerale SRSJ, Lucas LJ, & Keast RSJ. Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Current Opinion In Biotechnology 2012; 23(2): 129-135.
19. Laincer F, Laribi R, Tamendjari A, Arrar L, Rovellini P, & Venturini S. Olive oils from Algeria: Phenolic compounds, antioxidant and antibacterial activities. Grasas y Aceites 2014; 65(1): 001.
20. Alkan H, Baştan A, Salar S, Özdal M, & Kaymaz M. Kuru döneme çıkarken enfekte ve sağlıklı meme loblarında california mastitis test ve somatik hücre sayısı ile bakteriyolojik muayene sonuçlarının karşılaştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi 2014; 61(3): 179-183
21. Cobankara FK, Altinoz HC, Erganis O¸ Kav K, Belli S. In vitro antibacterial activities of root-canal sealers by using two different methods. Journal of Endodontics 2004; 30 (1): 57-60.
22. Rodriguez E, Gonzalez B, Gaya P, Nunez M and Medina M. Diversity of Bacteriocins Produced by Lactic Acid Bacteria Isolated From Raw Milk. International Dairy Journal 2000; 10: 7-15
23. Price L. Traditional use, modern research, Aromatherapy for health professionals E-book Shirley Price, & Len Price, Elsevier Health Sciences 2011; 77-130.
24. Azadi HG, Farzaneh N, Baghestani Z, Mohamadi A, & Shahri SAM. Effect of intramammary injection of Nigella sativa on somatic cell count and Staphylococcus aureus count in Holstein cows with S. aureus subclinical mastitis. American Journal of Animal and Veterinary Sciences 2011; 6(1): 31-34.
25. Yeşilmen S, Özyurtlu N, & Bademkıran S. Diyarbakır yöresinde subklinik mastitisli ineklerde etken izolasyonu ve duyarlı antibiyotiklerin belirlenmesi. Dicle Üniversitesi Veteriner Fakültesi Dergisi 2012; (1): 24-29.
26. Gülmez SA, Otlu S, Çoşkun, MR, Çelik, E, Büyük F, & Şahin M. İneklerde subklinik mastitis prevalansı, etken izolasyonu ve antibiyotik duyarlılıklarının belirlenmesi. Eurasian Journal of Veterinary Sciences 2018; 34(2): 92-98.
27. Doss A, Soris PT, & Mohan VR. Evaluation of antibacterial effect of medicinal plant extracts on bacteria isolated from subclinical bovine mastitis. International Journal of Veterinary Science and Agriculture Research 2019; 1(3): 1-8.
28. Paiano RB, Bonilla J, de Sousa RLM, Moreno AM, & Baruselli PS. Chemical composition and antibacterial activity of essential oils against pathogens often related to cattle endometritis. The Journal of Infection in Developing Countries 2020; 14(2):177-183.
29. Al Laham SA, & Al Fadel FM. Antibacterial efficacy of variety plants against the resistant Streptococcus which cause clinical mastitis in cows. Asian Journal of Pharmaceutical Research and Health Care 2013; 5(1): 32-41
30. Singh S, Malhotra M, & Majumdar DK. Antibacterial activity of Ocimum sanctum L. fixed oil. Indian Journal of Experimental Biology 2005; 43: 835-837.
31. Jayati AK, Bhatia AK, Kumar A, Goel A, Gupta A, & Rahal A. Antibacterial activity of hot aqueous extract of Ocimum sanctum leaves against common bacterial pathogens of animals. Pharma Science Monitor 2013; 3(3): 279-285.
32. Mukherjee R, Dash PK, & Ram GC. Immunotherapeutic potential of Ocimum sanctum (L) in bovine subclinical mastitis. Research In Veterinary Science 2005; 79(1): 37-43.
33. Aydin H. Phytotherapy with O. sanctum and O. onites in cows with subclinical mastitis. Kocatepe Veterinary Journal 2020; 13(4): 420-425.
34. Gupta R, Kumar S, & Khurana R. Essential oils and mastitis in dairy animals: a review. Haryana Vet. 2020; 59: 1-9.