Chemical characterization, evaluation of the antibacterial and antioxidant activity of the essential oil of Algerian (Myrtus communis l).

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Youcef Hadef
Abdelghani Djahoudi
Karim Hosni


Myrtus communis L, essential oil, chemical composition, Pathogenic Bacteria, antibacterial activity, antioxidant activity.


The essential oil extracted from Algerian  Myrtle were analysed by gas chromatography/mass spectrometry (GC/MS),  it revealed 67 components accounting for 99.07 % of the oil. the major components were α-pinene (24.83 %), 1,8-cineole (10.8 %), limonene (6.07 %), α-Terpineol (4.14%). Methyl eugenol (3.48%),  The in vitro antibacterial activity was performed  by agar disc diffusion and  (MIC) .The oil was tested against Staphylococcus aureus ,Staphylococcus aureus ATCC 29213,Staphylococcus aureus ATCC 25923, Methicillin-resistant  Staphylococcus aureus (MRSA) , Escherichia coli  ATCC  25922 ,  ciprofloxacin resistant Escherichia coli  (E coli cip R). Pseudomonas aeruginosa VIM 2,imipenem- resistant Pseudomonas aéruginosa (pseudomonas aeruginosa IMP), Pseudomonas aéruginosa ATCC 27853, Enterobacter cloacea , carbapenemase-negative Klebsiella pneumonia (KPC -) ,carbapenemase-positive Klebsiella pneumoniae (KPC+ ), Klebsiella pneumoniae , Acenitobacter baumannii.The antibacterial test revealed that myrtle oil showed a good antibacterial effect except against pseudomonas . The antioxidant activity of essential oil was evaluated by DPPH assay. The results showed the highest free radical scavenging activity with dpph ( 93.39 %)at the concentration of EO 4 mg/ml .


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1-Ben Ghnaya A , Chograni H , Messoud C , Boussaid M. Comparative Chemical -Composition and Antibacterial Activities of Myrtus communis L. Essential Oils Isolated from Tunisian and Algerian Population. J Plant Pathol Microb 2013; 4:7.
2-Emami SA, Asgary S, Naderi GA, Shams MR, Kasher T . Antioxidant activities of Juniperus foetidissima essential oils against several oxidative systems. Revista Brasileira de Farmacogno sia 2011 ;21:627-634.
3-lsmaili R, Fechtal M, Zine El Abidine A, Hachimi M, Sesbou A. Effet de Ia transplantation sur le rendement et Ia composition chimique du Myrte (Myrtus communis L.) Ann. Rech. For. Maroc 2001; 34: 87-93.
4-Atzei AD. Le piante nella tradizione popolare della Sardegna. Sassari, Italy: CarloDelfino Editore 2003; 319–23.
5-Bellakhdar J, Miki w and Honda G.Herboristes et pharmacopée à Marrakech et Salé (Maroc) AI Biruniya, Rev. Mar. Pharm 1994 ; 10 (1) : 26-27.
6-Sijelmassi A. Les plantes medicinales du Maroc. Ed. Le Fennec, Casablanca 1991 . 137 .
7-Lawrence B,M . Progress in essential oils. Myrtle oil. Perfumer and Flavorist 1996; 21: 57-58.
8-Chryssavgi G, Vassiliki P, Athanasios M, Kibouris T, Michael K. Essential oil compo-sition of Pistacia lentiscus L. and Myrtus communis L.: evaluation of antioxidant capacity of methanolic extracts. Food Chem 2008; 107:1120–30
9- Bouzouita N, Kachouri F, Hamdi M, Chaabouni M . Antimicrobial activity of essential oils from Tunisian aromatic plants. Flavour Frag J 2003; 18: 380-383.
10- Yadegarinia D, Gachkar L, Rezaei M B, Taghizadeh M, Shakiba A A, Rasooli I, Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils. Phytochemistry 2006; 67, 1249–1255 .
11-Tajkarimi M M, Ibrahim SA, Cliver DO. Antimicrobial herb and spice compounds in food. Food Control 2010 ;21:1199-1218.
12-Billerbeck VG, Roques C, Vaniére P, Marquier P . Activité antibactérienne et antifongique des produits à base d'huiles essentielles. Hygiénes X-n°3.2002 ;248-251
13-Mann C.M , Cox S.D , Markham J.L. The outer membrane of Pseudomonas aeruginosa NCTC 6749 contributs to its tolerance to the essential oil of Melaleuca alternifolia(Tea tree oil). Letters in Applied Microbiology 2000; 30: 294-297.
14- Hennia A, Brada M, Nemmiche S, Fauconnier M L, Lognay G. Chemical composition and antibacterial activitiy of the essential oils of Algerian Myrtus communis L . Journal of Essential Oil Research 2015; 27 (4): 324-328
15- Satrani B , Farah A, Talbi M. Effect of fractional distillation on chemical composition and antimicrobial activity essential oils of Myrtle (Myrtus communis L.) from Morocco. Acta Botanica Gallica 2006; 153:2, 235-242.
16-Wannes W A , Mhamdi B , Marzouk B .Variations in essential oil and fatty acid composition during Myrtus communisvar. Italic fruit maturation. FoodChemistry 2006; 112(3): 621-626.
17-Senatore F , Formisano C , Napolitano F, Rigano D, Ozcan M. Chemical composition and antibacterial activity of essential oil of Myrtus communis L. growing wild in Italy and Turkey. J. Essent. Oil Bearing Plants 2006; 9(2): 162-169.
18- Foudil-Cherif Y, Boutarene N, Yassaa N. Chemical composition of essential oils of Algerian Myrtus communis and chiral analysis of their leave volatiles. Journal of Essential Oil Research 2013;25:5, 402-408.
19- Bouzabata A, Cabral C, Goncalves M J et al., “Myrtus communis L. as source of a bioactive and safe essential oil,”Food and Chemical Toxicology,vol.75.2015;166–172.
20- Hammer K A, Carson C F , Riley T vitro activity of essential oils in particular Melaleuca alternifolia oil and tea tree oil products against candida Spp.J. antimicrob. chemother 1998;42:591-595.
21-Deans S G, Ritchie G. Antibacterial properties of plant essential oils. International Journal of Food Microbiology1987; 5: 165-180.
22- Rasooli I, Moosavi M L, Rezaee M B , Jaimand K. Susceptibility of microorganisms to Myrtus communis L. essential oil and its chemical composition. J. Agric. Food Chem 2002; 4, 127–133 .
23- Hateet R R , Khadhim A, Shawi H H . Biological Activity of Eugenol Acetate as Antibacterial and Antioxidant Agent, Isolation from Myrtus communisL. Essential Oil. International Journal of Bioengineering & Biotechnology 2016; 1(2): 6-11.
24-Marino M, Bersani C, Comi G . Impedence measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositeae. Int. J. Food Microbiol 2001; 67:187-195
25-Stojkovic D, Sokovic M D ,Glamoclija J, Dzamic A, Cirié A, Ristié M, et al. Chemical composition and antimicrobial activity of vitex agnus castus L, fruits and leaves essential oils ,food chemistry2011 ;128 :1017-1022.
26- Snoussi A, Essaidi I, Ben Haj koubaierh, Chaabouni M C, Bouzouita N. Chemical composition and antioxidant activity of essential oils and ethanol extracts of myrtus communis l. Organs (berries, leaves and floral buds) journal de la société chimique de tunisie, 2012; 14: 69-76
27-Berka-Zougali B , Ferhat MA , Hassani A , Chemat F, Allaf K S. Comparative study of essential oils extracted from Algerian Myrtus communis L. leaves using microwaves and hydrodistillation. Int J Mol Sci 2012 ;13 : 4673–95
28-Boukhris M, Regane G, Yangui T, Sayadi S, Bouaziz M . Chemical composition and biological potential of essential oil from Tunisian Cupressus sempervirens L. Journal of Arid Land Studies 2012;22:329-332.

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