Effects of quercetin and catechin on blood lactate level

Main Article Content

Meral Miyaç Göktepe
Mehmet Göktepe
Erdil Durukan
Mehmet Günay
Ferhat Güder
Meriç Eraslan

Keywords

Antioxidants, Flavonoids, Quercetin, Catechin, Exercise, Lactate

Abstract

Study Objectives: This study was aimed to investigate the effects of combined administration of Catechin and Quercetin on blood lactate level. Methods: The research was conducted on 12 male Wistar rats weighing 300-350 gr. Rats were divided into two groups as the control group (n=6) and the test group (n=6). After the rats were quarantined for 10 days; each rat heart blood was taken on the first test day (pre catechin+quercetin supplementation), and again after they were participated to exercise on the second test day (pre catechin+quercetin supplementation). Between days 3 and 12 after testing (for 10 days), each rat in the test group was given 20 ml/kg of catechin+quercetin once daily, and each rat in the control group was given 1ml/kg of 0.05% Dimethyl Sulfoxide (DMSO) by gavage. On days 13 (post catechin+quercetin supplementation), each rat was participated to exercise and take their heart blood. On day 14, the animals were allowed to rest and on day 15 (post catechin+quercetin supplementation), rats’ heart blood were taken under anesthesia. Mann Whitney test was used to analyze data. Results: There was no significant difference between control and test groups during the rest day and during exercise (day 2) testing. Significant changes were observed according to the results of the rats, which were practiced to exercise after administration of catechin+quercetin for 10 days, between day 3 and day 12 after testing. A significant reduction was determined in lactate levels (p<0.01). Conclusion: Combined administration of catechin+quercetin was found to reduce lactate levels during exercise, suggesting a performance-enhancing effect.

Downloads

Download data is not yet available.
Abstract 17 | PDF Downloads 5

References

1. Ji LL. Leeuwenburgh C, Leichtweis S, et al. Oxidative stress and aging: role of exercise and its influences on antioxidant systems. Ann Ny Acad Sci. 1998; 854: 102-17.
2. Meisler A, Anderson ME, Glutathione. Ann Rev Biochem. 1983; 52, 711-60.
3. Aydın A, Sayal A, Işımer A. Free Radicals and Antioxidant Protection System. Ankara: Gülhane Military Academy of Medicine Printing House No:20; 2001.
4. Gerhauser C. Beer constituents as potential cancer chemopreventive agents. Eur J Cancer. 2005; 41: 1941-1954.
5. Ayla S, Oktar H, Tanrıverdi G. et al. Protective Effect of Nicotinamid on Doxorubicin-induced Rat Hepatotoxicity . Anadolu University Science and Technology Journal. 2009; 10(1): 229-238.
6. Erkoç Ş, Erkoç F, Keskin N. Theoretical investigation of quercetin and its radical isomers. J Mol Struct. 2003; 631: 141-6.
7. Chen JC, Ho FM, Pei-Dawn Lee Chao, et al. Inhibition of iNOS gene expression by quercetin is mediated by the inhibition of IkappaB kinase, nuclear factor-kappa B and STAT1, and depends on heme oxygenase-1 induction in mouse BV-2 microglia. Eur J Pharmacol. 2005; 521(1-3): 9-20.
8. Schirmer RH, Krauth-Siegel RL, Schulz GE. Glutathione Reducase, New York, John Wiley and Sons Press. 1989.
9. Smith JEW, Pirner M, Zachwieja Jeffrey JFACSM. Quercetin Lowers Blood Lactate Response During Progressively Intense Exercise. Med Sci Sports Exerc. 2011; 43(5): 430.
10. Gasparin FR, Spitzner FL, Ishii-Iwamoto EL, et al. Constantin J. Actions of quercetin on gluconeogenesis and glycolysis in rat liver. Xenobiotica; 2003; 33(9): 903-11.
11. Su-juan C, Hai-ying L, Zheng-ying X. Effect of quercetin on hemoglobin and blood biochemical indexes in rats following exhausted exercise. Journal of Clinical Rehabilitative Tissue Engineering Research. 2007; 11 (37): 7468-9.
12. Gai-ning D. Effect of quercetin on Free radical Metabolism in Muscle and Exercise Performance of Trained Rat. Journal of Clinical Rehabilitative Tissue Engineering Research. 2006; 23(2): 24-33.
13. Adewole SO, Caxton-Martins EA, Ojewole JAO. Protective Effect of quercetin on the Morphology of Pancreatic β-Cells of Streptozotocin-Treated Diabetic Rats. Afr J Tradit Complement Altern Med. 2007; 4(1): 64–74.
14. Duarte J, Pérez-Palencia R, Vargas F, et al. Antihypertensive effects of the flavonoid quercetin in spontaneously hypertensive rats. Br J Pharmacol. 2001; 133(1): 117-24.
15. Phachonpai W, Wattanathorn J, Muchimapura S, et al. Neuroprotective effect of quercetin encapsulated liposomes: a novel therapeutic strategy against Alzheimer's disease. Am J Appl Sci. 2010; 7(4); 480-5.
16. Davis JM, Murphy EA, Carmichael MD, Davis B. quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance. Am J Physiol Regul Integr Comp Physiol. 2009: 296(4): 1071-7.
17. Nieman DC, Williams AS, Shanely RA, et al. quercetin’s influence on exercise performance and muscle mitochondrial biogenesis. Med Sci Sports Exerc. 2010; 42(2): 338-45.
18. Bakheet SA. Assessment of anti-cytogenotoxic effects of quercetin in animals treated with topotecan. Oxid Med Cell Longev, 2011.
19. Baldassari J, Franke WC, Horohov DW, et al. Effects of quercetin on exercise potential and exercise-induced cytokines in the horse. Comp Exer Physiol. 2012; 8(2): 131-42.
20. Alessio HM, Hagerman AE, Fulkerson BK, et al. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000; 32(9): 1576-81.
21. Astrand PO, Rodahl K, Dahl HA, et al. Textbook of Work Physiology Physiological Bases of Exercise, Fourth Edition, Canada: Human Kinetics Pub; 2003.
22. Murase T, Haramizu S, Shimotoyodome A, et al. Green tea extract improves running endurance in mice by stimulating lipid utilization during exercise. Am J Physiol Regul Integr Comp Physiol 2006; 290(6): 1550–6.
23. Fraga CG, Actis-Goretta L, Ottaviani JI, et al. Regular consumption of a flavanol-rich chocolate can improve oxidant stress in young soccer players. Clinical & Developmental Immunology, March 2005; 12(1): 11–7.