Intermediate products of purine metabolism in an experimental model of pancreatic necrosis Purine bases as predictors of pancreatic necrosis

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Yelena Pozdnyakova
Grigoriy Abramov https://orcid.org/0000-0002-9532-108X
Neila Tankibaeva https://orcid.org/0000-0002-7432-6535
Kairat Shakeev https://orcid.org/0000-0002-7802-1464
Maida Tusupbekova https://orcid.org/0000-0003-3105-4450
Dmitriy Shestakov https://orcid.org/0000-0001-7251-093X

Keywords

purines, purine metabolism intermediates, predictors of necrosis, pancreas, pancreatitis, pancreatic necrosis

Abstract

Background and aim: To determine the level of purines in the blood plasma of experimental animals at three stages of induced pancreatic necrosis and to establish the potential of purines as predictors for predicting the severity of pancreatitis.


Methods: Outbred rabbits were involved in the experiment. Modelling of pancreatic necrosis by means of the auto-bile injection into the pancreatic parenchyma was done. At the time of withdrawal from the experiment, the rabbit pancreas was isolated with a microscopic description, blood was taken to identify the levels of adenine, guanine, hypoxanthine, xanthine and uric acid in the blood plasma.


Results: 12 hours after the administration of auto-bile, the level of xanthine significantly increases and the concentration of uric acid in the blood plasma increases by 3 times. 24 hours after the introduction of auto-bile, there is a slight decrease in the level of adenine, xanthine and uric acid, and the indicators of purine metabolism remain elevated. 48 hours after the introduction of auto-bile, the levels of guanine, hypoxanthine and xanthine are reduced.


Conclusions: The concentration indices of absolute and relative intermediate products of purine metabolism were increased at the initial stage of pancreatic necrosis. The activity of enzymes and metabolites of purine metabolism involved in the formation of reactive oxygen species and free radicals increased. The hypothesis that intermediate products of purine metabolism can be predictors of pancreatic necrosis was confirmed.

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