Biochemical, immunochemical and serology analytes validation of the lithium heparin BD Barricor blood collection tube on a highly automated Roche COBAS8000 instrument

Main Article Content

Davide Ferrari
Marta Strollo
Matteo Vidali
Andrea Motta
Marina Pontillo
Massimo Locatelli

Keywords

blood collection tubes, mechanical separator, plasma, serum, serology

Abstract

Background. Recently developed blood tubes with a barrier to provide plasma are becoming widespread. We compared 43 biochemical, 35 immunochemical and 7 serology analytes in a BD-Vacutainer® Barricor tube for local clinical validation of this lithium-heparin tube with a barrier.


Methods. Samples from 70 volunteers were collected in different BD-tubes: a clot-activator tube with gel (SST), a lithium-heparin tube with gel (PST), and a lithium-heparin tube with barrier (BAR). Biases from Bland-Altman plots and 95% confidence intervals were compared with the desirable specification from the Ricos database in order to verify whether measurements from different tubes were significantly different.


Results. For most of the analytes tested, the measurements using SST, PST or BAR tubes were equivalent. Only BIC, GLU, K, LAD, LPA, P, TP, CTX, Ferritin, HGH, vitD3 and ANTIS showed statistically significant, between-tubes, differences which might have clinical implication.


Conclusions. The study demonstrates that SST, PST and BAR can be used interchangeably for most of the analytes tested, including serology analytes. This allows the use of the same tube for assaying multiple analytes, increasing the laboratory efficiency while decreasing patients discomfort by minimizing blood withdrawal.

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References

1. Bowen RAR, Hortin GL, Csako G, Otañez OH, Remaley AT. Impact of blood collection devices on clinical chemistry assays. Vol. 43, Clinical Biochemistry. 2010. p. 4–25.
2. Arslan FD, Karakoyun I, Basok BI, Aksit MZ, Baysoy A, Ozturk YK, et al. The local clinical validation of a new lithium heparin tube with a Barrier: BD vacutainer® barricor LH plasma tube. Biochem Medica. 2017;27(3).
3. Cadamuro J, Mrazek C, Leichtle AB, Kipman U, Felder TK, Wiedemann H, et al. Influence of centrifugation conditions on the results of 77 routine clinical chemistry analytes using standard vacuum blood collection tubes and the new bd-barricor tubes. Biochem Medica. 2018;28(1).
4. Padoan A, Zaninotto M, Piva E, Sciacovelli L, Aita A, Tasinato A, et al. Quality of plasma samples and BD Vacutainer Barricor tubes: Effects of centrifugation. Clin Chim Acta. 2018;483:271–4.
5. Dupuy AM, Badiou S, Daubin D, Bargnoux AS, Magnan C, Klouche K, et al. Comparison of BarricorTM vs. lithium heparin tubes for selected routine biochemical analytes and evaluation of post centrifugation stability Anne. Biochem Med. 2018;28(2):1–7.
6. Ferrari D, Manca M, Premaschi S, Banfi G, Locatelli M. Toxicological investigation in blood samples from suspected impaired driving cases in the Milan area : Possible loss of evidence due to late blood sampling. Forensic Sci Int. 2018;288:211–7.
7. Ferrari D, Manca M, Banfi G, Locatelli M. Alcohol and illicit drugs in drivers involved in road traffic crashes in the Milan area. A comparison with normal traffic reveals the possible inadequacy of current cut-off limits. Forensic Sci Int. 2018;282:127–32.
8. Ferrari D, Lombardi G, Strollo M, Pontillo M, Motta A, Locatelli M. Association between solar ultraviolet doses and vitamin D clinical routine data in European mid-latitude population between 2006 and 2018. Photochem Photobiol Sci. 2019;18(11):2696–706.
9. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Int J Nurs Stud [Internet]. 2010;47(8):931–6. Available from: http://dx.doi.org/10.1016/j.ijnurstu.2009.10.001
10. WESTGARD J. Westgard QC [Internet]. 2014. Available from: https://www.westgard.com/
11. Er T-K, Tsai L-Y, Jong Y-J, Chen B-H. Selected Analyte Values in Serum Versus Heparinized Plasma Using the SYNCHRON LX PRO Assay Methods/Instrument. Lab Med. 2006;37(12):731–2.

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