Interleukin-10 and Transforming Growth Factor Beta1 Gene Polymorphisms in Chronic Heart Failure

Mohammad Jafar Mahmoudi; Mona Hedayat; Mohammad Taghvaei; Sara Harsini; Ebrahim Nematipour; Nima Rezaei; Elham Farhadi; Maryam Mahmoudi; Maryam Sadr; Nilufar Esfahanian; Keramat Nourijelyani; Ali Akbar Amirzargar

doi:10.23750/abm.v90i2.6681

Interleukin-10 and Transforming Growth Factor Beta1 Gene Polymorphisms in Chronic Heart Failure

Authors

Mohammad Jafar Mahmoudi
Mona Hedayat Division of Immunology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA
Mohammad Taghvaei Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Sara Harsini Tehran University of Medical Sciences
Ebrahim Nematipour Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
Nima Rezaei
Elham Farhadi Hematology Department, School of Allied Medical Science, Tehran University of Medical Sciences, Tehran, Iran; 7 School of Nutrition and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Maryam Mahmoudi School of Nutrition and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
Maryam Sadr Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Nilufar Esfahanian Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Keramat Nourijelyani Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Ali Akbar Amirzargar Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran

DOI: https://doi.org/10.23750/abm.v90i2.6681

Keywords:

Heart failure, Single nucleotide polymorphism, Interleukin-10, Transforming growth factor beta1

Abstract

Background: As cytokines, including interleukin-10 (IL-10) and transforming growth factor beta 1(TGF-β1) seem to contribute towards the pathogenesis of chronic heart failure (CHF), this study was performed to assess the associations of certain single nucleotide polymorphisms (SNPs) of these genes in a case control study. Methods: This investigation was carried out to determine the frequency of alleles, genotypes and haplotypes of TGF-β1 and IL-10 single-nucleotide polymorphisms (SNPs) in 57 Iranian patients with CHF compared with 140 healthy subjects using polymerase chain reaction with sequence-specific primers method. Results: Results of the analyzed data divulged a negative association for both TGF-β1 GC genotype at codon 25 (P=0.047) and CT genotype at codon 10 (P=0.018) and CHF proneness. Although, TGF-β1 CC genotype at codon 10 was found to be positively associated with CHF (P=0.011). Moreover, the frequency of IL-10 (−1082, -819, -592) ATA haplotype and TGF-β1 (codon 10, codon 25) TG haplotype were significantly lower in the patients group (P=0.004 and P=0.040, respectively), while TGF-β1 (codon 10, codon 25) CG haplotype was overrepresented in patients with CHF (P=0.007). Conclusions: Cytokine gene polymorphisms might affect vulnerability to CHF. Particular genotypes and haplotypes in IL-10 and TGF-β1 genes could render individuals more susceptible to CHF.