Molecular epidemiology of SEN virus among blood donors and renal dialysis patients

Thekra Almaqati; Elmoeiz A.  Elnagi; Faisal  Al Zahrani; Muzaheed; Zeinab S.  Mohammed; Amer  Alomar; Siju K  George; Ashly Mary   Jacob; Fahad A  Mashwal

doi:10.23750/abm.v93i5.13005

Molecular epidemiology of SEN virus among blood donors and renal dialysis patients

Authors

Thekra Almaqati Clinical Laboratory Sciences Department, Prince Sultan Military College of Health Sciences, Dhahran, Saudi Arabia https://orcid.org/0000-0001-5705-2053
Elmoeiz A. Elnagi
Faisal Al Zahrani Department of Clinical Laboratory Science, College of applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam 31441 Saudi Arabia
Muzaheed Department of Clinical Laboratory Science, College of applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam 31441 Saudi Arabia https://orcid.org/0000-0002-2814-8205
Zeinab S. Mohammed Faculty of Clinical Laboratory Sciences, Alzaiem Alazhari University, Khartoum, Sudan
Amer Alomar Department of Clinical Laboratory Science, College of applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam 31441 Saudi Arabia
Siju K George Clinical Laboratory Sciences Department, Prince Sultan Military College of Health Sciences, Dhahran, Saudi Arabia.
Ashly Mary Jacob Clinical Laboratory Sciences Department, Prince Sultan Military College of Health Sciences, Dhahran, Saudi Arabia.
Fahad A Mashwal Clinical Laboratory Sciences Department, Prince Sultan Military College of Health Sciences, Dhahran, Saudi Arabia.

DOI: https://doi.org/10.23750/abm.v93i5.13005

Keywords:

SEN-V, SENV-D, SENV-H, dialysis, blood, blood donor, RNA virus

Abstract

Background and purpose: The SEN virus (SEN-V) is a single-stranded circular, non-enveloped DNA virus that has been linked to blood transfusion and is thought to be a major cause of post-transfusion hepatitis. The two SENV types, SENV-H and SENV-D, are non-A to E hepatitis viruses in those who are infected. The purpose of this study is to find out how common SENV and its variations are among renal dialysis patients and healthy blood donors.

Methods: The study used a cross-sectional design, with 300 blood samples collected from KFMMC patients, 150 from healthy blood donors and 150 from renal dialysis patients, between January 2019 and January 2021. The samples were screened for the presence of SENV-D and SENV-H. using nested PCR.

Results: Molecular analysis of the SEN virus revealed that 9.3% of the samples (14 out of 150) tested positive for SEN virus infection in renal dialysis patients. The data from healthy donors revealed that 10% of the samples tested positive for the SEN virus (15 out of 150).

Conclusions: The presence of SEN-V in healthy blood donors and renal dialysis patients demonstrates the virus's blood-borne nature and emphasizes the dangers of blood-borne transmission.

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