Chronically Pre-treatment of Pistachio Alters the Morphology of CA1 Hippocampus Neurons Following Transient Focal Cerebral Ischemia in Male Wistar Rats

Chronically Pre-treatment of Pistachio Alters the Morphology of CA1 Hippocampus Neurons Following Transient Focal Cerebral Ischemia in Male Wistar Rats


  • Hossein Pourmobini Department of anatomy, Afzalipour Faculty of medicine, Kerman University of Medical Sciences, Kerman, Iran
  • Mohammad Reza Afarinesh 3Student Research Committee, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
  • Ali Shamsara Department of anatomy, Afzalipour Faculty of medicine, Kerman University of Medical Sciences, Kerman, Iran
  • Seyed Hasan Eftekhar-Vaghefi 1- Kerman Neuroscience Research Center, Institute of Neuropharmachology, Kerman University of Medical Sciences, Kerman, Iran 2- Department of Anatomy, Kerman Branch, Islamic Azad University, Kerman, Iran
  • Majid Asadi-Shekaari Kerman Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran


Pistacia vera, Stroke, Antioxidant, Anti-inflammatory, Cerebral ischemia.


Background: Consumption of antioxidants is effective on reducing the damage caused by cerebral ischemia. Objectives: We investigated the effect of Pistacia vera (pistachio) pretreatment on the morphology of the cornu ammonis (CA1) region of hippocampus neurons of the rats' hippocampus following transient focal cerebral occlusion of the middle cerebral artery (MCA). Methods: In this study, 30  male Wistar rats were divided into 3 groups of control, ischemia, and pretreatment with pistachio (fed with pistachio at 6% of the diet for a five-week duration before the right MCA occlusion). Neurological scores of the rats were assessed using Baderson rating. Thereafter, the animals’ balance and muscle power were assessed by Rotarod and forelimb wire-grip strength tests, respectively. Finally, histopathological and morphometrical characteristics of hippocampal neurons were studied using Hematoxylin-Eosin method. Results: Neurological scores of the ischemia group significantly decreased compared to the control group (p<0.05), while pretreatment with pistachio significantly improved Baderson rating scores compared to the ischemia group (p<0.05). Although stroke significantly decreased the balance and muscular strength in the studied rats compared to the normal rats (p<0.05), pistachio’s exposure significantly increased the balance and muscular strength compared to the ischemia group (p<0.05). Additionally, a significant decrease was observed in the volume of stroke and neuronal degradation in the pistachio-treated rats compared with the ischemia group (p<0.05). Conclusions: Pistachio consumption reduces the volume of infarction and neuronal damage and improves neurological disorders after ischemia. Therefore, pretreatment with pistachio would have a protective effect against stroke.


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How to Cite

Pourmobini H, Afarinesh MR, Shamsara A, Eftekhar-Vaghefi SH, Asadi-Shekaari M. Chronically Pre-treatment of Pistachio Alters the Morphology of CA1 Hippocampus Neurons Following Transient Focal Cerebral Ischemia in Male Wistar Rats. Acta Biomed [Internet]. 2022 Jan. 19 [cited 2024 Jul. 13];92(6):e2021394. Available from: