Seroprevalence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection in an Italian cohort in Marche Region, Italy

Main Article Content

Mauro De Santi
Aurora Diotallevi
Giorgio Brandi


COVID-19, SARS-CoV-2 antibodies, seroprevalence, Italy, risk factors


Background and aim: The COVID-19 is an infectious disease caused by the novel coronavirus SARS-CoV-2, declared a public health emergency by the World Health Organization. In this study, we evaluated the seroconversion of SARS-CoV-2 antibodies to find predictors of infection in terms of symptoms, health status, and professions.

Methods: Serological samples of 341 volunteers in a cohort in Marche Region, Italy, were analyzed for the presence of IgM and/or IgG immunoglobulins specific for the SARS-CoV-2. Contextually, an anamnestic questionnaire was administered. The binary logistic regression analysis was used to find the predictors of seroconversion.

Results: Forty-nine subjects (14.4 %) were found positive, without significant differences between gender and age groups. The predictors identified inside the variable categories “symptoms,” “risk factors” (smoking habit and established pathologies), and “professions” were the loss of taste and smell (OR, 8.563), cardiovascular diseases (OR, 2.912), and policeman profession (OR, 3.875), respectively.

Conclusions: Although the limited number of subjects recruited in this study, our results could give important findings to be considered for planning preventive strategies in the view of the next COVID-19 waves.


Download data is not yet available.


Metrics Loading ...
Abstract 23 | PDF Downloads 7


1. WHO. Coronavirus disease (COVID-19) outbreak [Internet]. 2020 [cited 2020 Mar 4]. Available from:
2. Dimcheff DE, Schildhouse RJ, Hausman MS, Vincent BM, Markovitz E, Chensue SW, et al. Seroprevalence of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) Infection Among VA Healthcare System Employees Suggests Higher Risk of Infection When Exposed to SARS-CoV-2 Outside of the Work Environment. Infect Control Hosp Epidemiol [Internet]. 2020 Sep 23 [cited 2020 Oct 8];1–25. Available from:
3. Cacciapaglia G, Cot C, Sannino F. Second wave COVID-19 pandemics in Europe: a temporal playbook. Sci Rep [Internet]. 2020 Dec 1 [cited 2020 Oct 8];10(1). Available from:
4. Middleton J, Lopes H, Michelson K, Reid J. Planning for a second wave pandemic of COVID-19 and planning for winter: A statement from the Association of Schools of Public Health in the European Region [Internet]. International Journal of Public Health. Springer; 2020 [cited 2020 Oct 8]. Available from:
5. Long QX, Liu BZ, Deng HJ, Wu GC, Deng K, Chen YK, et al. Antibody responses to SARS-CoV-2 in patients with COVID-19. Nat Med [Internet]. 2020 Jun 1 [cited 2020 Oct 8];26(6):845–8. Available from:
6. Iyer AS, Jones FK, Nodoushani A, Kelly M, Becker M, Slater D, et al. Persistence and decay of human antibody responses to the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 patients. Sci Immunol [Internet]. 2020 Oct 8 [cited 2020 Oct 13];5(52):eabe0367. Available from:
7. Amendola A, Tanzi E, Folgori L, Barcellini L, Bianchi S, Gori M, et al. Low seroprevalence of SARS-CoV-2 infection among healthcare workers of the largest children hospital in Milan during the pandemic wave. Infect Control Hosp Epidemiol [Internet]. 2020 [cited 2020 Oct 8]; Available from:
8. Fill Malfertheiner S, Brandstetter S, Roth S, Harner S, Buntrock-Döpke H, Toncheva AA, et al. Immune response to SARS-CoV-2 in health care workers following a COVID-19 outbreak: A prospective longitudinal study. J Clin Virol [Internet]. 2020 Sep 1 [cited 2020 Oct 8];130. Available from:
9. Sotgiu G, Barassi A, Miozzo M, Saderi L, Piana A, Orfeo N, et al. SARS-CoV-2 specific serological pattern in healthcare workers of an Italian COVID-19 forefront hospital. BMC Pulm Med [Internet]. 2020 Jul 29 [cited 2020 Oct 8];20(1). Available from:
10. Calcagno A, Ghisetti V, Emanuele T, Trunfio M, Faraoni S, Boglione L, et al. Risk for SARS-CoV-2 Infection in Healthcare Workers, Turin, Italy. Emerg Infect Dis [Internet]. 2020 Oct 6 [cited 2020 Oct 14];27(1). Available from:
11. Squeri R, Levita A, Intelisano R, Costa GB, Mancuso G, Grasso L, et al. Correct management and low rate of contagiousness of healthcare workers in a university hospital in Southern Italy: From contact tracing to serological investigation. Acta Biomed [Internet]. 2020 [cited 2020 Oct 14];91(9-S):79–86. Available from:
12. Steensels D, Oris E, Coninx L, Nuyens D, Delforge ML, Vermeersch P, et al. Hospital-Wide SARS-CoV-2 Antibody Screening in 3056 Staff in a Tertiary Center in Belgium [Internet]. Vol. 324, JAMA - Journal of the American Medical Association. American Medical Association; 2020 [cited 2020 Oct 8]. p. 195–7. Available from:
13. Wang X, Ferro EG, Zhou G, Hashimoto D, Bhatt DL. Association between Universal Masking in a Health Care System and SARS-CoV-2 Positivity among Health Care Workers [Internet]. Vol. 324, JAMA - Journal of the American Medical Association. American Medical Association; 2020 [cited 2020 Oct 8]. Available from:
14. Li R, Pei S, Chen B, Song Y, Zhang T, Yang W, et al. Substantial undocumented infection facilitates the rapid dissemination of novel coronavirus (SARS-CoV-2). Science (80- ) [Internet]. 2020 May 1 [cited 2020 Oct 14];368(6490):489–93. Available from:

DB Error: Unknown column 'Array' in 'where clause'