Cytokine gene polymorphisms in Pigeon Breeder’s Disease expression

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Cláudia Freitas
Bruno Lima
Natália Martins
Natália Melo
Patricia Mota
Helder Novais-Bastos
Helena Alves
Oksana Sokhatska
Luís Delgado
António Morais

Keywords

Pigeon breeder’s disease, hypersensitivity pneumonitis, cytokines, genetic polimorphisms

Abstract

Background: Exaggerated immunological response to repeated inhalation of organic or chemical dusts may lead to Hypersensitivity Pneumonitis among sensitized individuals. Only a few exposed individuals became ill and disease expression pattern is highly variable which suggest that genetic factors may play a role.


Objective: To investigate interferon (IFN)-γ, tumour necrosis factor (TNF)-α, interleukin (IL)-6, transforming growth factor (TGF)-ß, and IL-10 gene polymorphisms in a cohort of pigeon breeder’s disease (PBD) patients in comparison with asymptomatic exposed controls and the association with different patterns of disease.


Methods: We evaluated 40 PBD patients and 70 exposed controls. IFN-γ, TNF-α, IL-6, TGF-ß, and IL-10 polymorphisms were determined by polymerase chain reaction–sequence specific primer amplification.


Results: Polymorphism analysis of IFN-γ, TNF-α, IL-6, TGF-ß, and IL-10 genotypes and allele frequencies showed no differences between patients and controls. IFN-γ T/T genotype frequency was increased among patients with chronic presentation (RR=2.33, p=0.047) compared with those with acute/subacute presentation. Also, chronic presenting patients had an increased frequency of IFN-γ T allele (50% vs 22.5%, RR=1.76, p=0.011). No differences were found in TNF-α, IL-6, TGF-ß, and IL-10 genotypes neither allelic frequencies between both groups of patients. IL-6 C/C genotype was more frequent in patients who showed chronic evolution (RR=2.54, p=0.017), when comparing with patients with disease resolution.


Conclusion: IFN-γ T/T and the IL-6 C/C genotypes seem to play a role in HP expression, as their frequencies are increased in chronic presentations or in those with chronic evolution one year after the initial diagnosis, respectively.

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