Human embryonic stem cells recover in vivo acute lung inflammation bleomycin-induced

Human embryonic stem cells recover in vivo acute lung inflammation bleomycin-induced

Authors

  • Federica Sangiuolo Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy
  • P. Spitalieri Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy
  • M. C. Quitadamo Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy
  • A. Orlandi Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy
  • E. Puxeddu Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy
  • G. Curradi Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy
  • F Sangiuolo Department of Biomedicine and Prevention, Tor Vergata University of Rome, Rome, Italy

Keywords:

intranasal bleomycin administration, lung fibrosis mouse model, human embryonic stem cells, cell therapy.

Abstract

Idiopathic pulmonary fibrosis (IPF)  is characterized by alveolar epithelial cell injury, type II cell activation, apoptosis and bronchiolar epithelial cell proliferation, accumulation of extracellular matrix and fibroblasts. No current animal model recapitulates all of these cardinal manifestation of the human disease. However, bleomycin instillation in mice lung by intranasal way (ITN) represents the best experimental model of pulmonary fibrosis in which alveolar pneumocytes type II (ATII) are usually depleted. The aim of this study was to test the possibility to recover acute lung fibrosis after transplantation of human embryonic type II derived-pneumocytes in a murine model of bleomycin-induced damage. Our results indicate the striking “clinical” beneficial effect of differentiated HUES-3 cells into ATII in terms of lung function, weight loss and mortality in injured mice, suggesting this stem cell therapy as a promising, systemic and specific treatment of human pulmonary fibrosis.

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Published

22-11-2013

Issue

Vol. 30 No. 3 (2013)

Section

Original Articles: Laboratory Research

License

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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How to Cite

1.
Sangiuolo F, Spitalieri P, Quitadamo MC, Orlandi A, Puxeddu E, Curradi G, et al. Human embryonic stem cells recover in vivo acute lung inflammation bleomycin-induced. Sarcoidosis Vasc Diffuse Lung Dis [Internet]. 2013 Nov. 22 [cited 2025 Apr. 10];30(3):177-85. Available from: https://mattioli1885journals.com/index.php/sarcoidosis/article/view/2836