The toxicity of carbon dioxide inhalation

The toxicity of carbon dioxide inhalation

Authors

  • Laurent Schwartz Service de radiothérapie, Hôpital Pitié-Salpêtrière, Paris, France
  • Mohammad Abolhassani Nosco Pharmaceuticals, Paris, France
  • Adelina Guais Biorébus, Paris, France
  • Gianfranco Baronzino Radio-Hyperthermia Service, Hospital of Monza, Monza, Italy
  • Georges Grévillot Laboratoire des Sciences du Génie Chimique CNRS – ENSIC, Nancy, France
  • Philippe Chaumet-Riffaud Université Paris-Sud, EA4046, UFR de Bicêtre, Le Kremlin-Bicêtre, France AP-HP, CHU de Bicêtre, Service de Biophysique et de Médecine nucléaire, Le Kremlin-Bicêtre, France
  • Annie J. Sasco Epidemiology for Cancer Prevention, Inserm U897, Bordeaux School of Public Health, Victor Segalen Bordeaux 2 University, Bordeaux, France

Keywords:

cancer, carbon dioxide, inflammation, Protein Phosphatase 2A, NF-κB, tobacco smoke

Abstract

Carbon dioxide (CO2) is naturally present in the atmosphere where its concentration is about 0.038%. It is also a normal constituent of the body arising from cellular respiration or taking part in several metabolic reactions. CO2 is also one of the main side-products of combustion, and in particular of cigarette smoking. Analysis of primary tobacco smoke demonstrates the presence of a high concentration at 13.5% CO2. We designed a study to assess if CO2, either obtained from gas canister or from cigarette combustion, is pro-inflammatory. We analyzed in vitro the inflammation response induced by exposure to CO2 for 48 hours (0 to 20% with a constant O2 concentration of 21%). In addition, we carried out an in vivo experiment where mice were submitted to increasing concentrations of CO2 (0, 5, 10, 15% with a constant O2 concentration of 21%) for one hour. The exposure to concentrations above 5% of CO2 resulted in the dosedependent secretion of pro-inflammatory cytokines by A549 cells and pulmonary cells retrieved from the exposed mice as demonstrated by ELISA. Lung inflammation was also demonstrated in vivo by airway hyperreactivity induction pointed out by plethysmography. This response was mediated by the nuclear translocation of p65 Nuclear Factor kappa-B (NF-κB), itself a consequence of Protein Phosphatase 2A (PP2A) activation. Short inhibiting RNAs (siRNAs) targeted toward catalitic subunit PP2Ac prevented the effect of CO2, i.e. disrupted the NF-κB activation and the pro-inflammatory cytokine secretion. Thus, in vivo or in vitro exposure to high CO2 concentrations leads to an inflammation response mediated by the PP2A/NF-κB pathway. These results strongly suggest that exposure to carbon dioxide may be more toxic than previously thought. Because of the described relationship inbetween inflammation and cancer this data may be relevant for carcinogenic effects of combustion products such as those of tobacco.

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Published

2009-12-01

Issue

Section

Articles on original studies and research

How to Cite

1.
Schwartz L, Abolhassani M, Guais A, Baronzino G, Grévillot G, Chaumet-Riffaud P, et al. The toxicity of carbon dioxide inhalation. Eur J Oncol Env Hea [Internet]. 2009 Dec. 1 [cited 2025 Apr. 2];14(4):199-208. Available from: https://mattioli1885journals.com/index.php/EJOEH/article/view/3884