The Lady of Pavia: microbiological essays and qualitative GC/MS characterization of embalming materials

Main Article Content

Marco Nicola
Luca Maraviglia
Riccardo Ariotti
Francesco Chiara
Sabina Malgora

Keywords

mummification, embalming materials, microbiological essays, GC/MS, terpenoids

Abstract

The application of molecular characterization techniques to an Egyptian mummy preserved in the Archaeological Museum of the University of Pavia, a as part of a wide research project conducted by the Mummy Project Research, provided a description of the embalming technique adopted. The number of analytical methods applied has also made it possible to identify the presence of some molecules attributable to plants not included among those commonly reported in similar cases. Before the study, a microbiological screening of ten zones on the surface of the mummy revealed the effective antiseptic properties of the substances present. The chemical composition of the balms was then investigated using quadrupole Gas Chromatography/Mass Spectrometry (GC/MS), testing the effects of different extraction solvents and the use of a derivatizing agent, which allowed to improve the detection of some compounds. Overall, the compounds identified are linked to an embalming technique that involves the use of bitumen, oils and resins as common both in the Late and in the Ptolemaic Periods. Some analytical methods applied have also made it possible to detect traces of compounds possibly attributable to some genera of plants used by the Egyptians and which grow on the Mediterranean coast of North Africa or in southern tropical regions. The work, still ongoing, highlights the importance of using different analytical methods to detect even minor amounts of compounds difficult to identify. This approach aims at the improvement of the understanding of medical and embalming techniques used in ancient Egypt.

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References

Abdel-Maksoud, G., & El-Amin, A. R. (2011) A Review on the Materials Used During The Mummification Processes in Antient Egypt. Mediterranean Archaeology and Archaeometry 11 (2), 129–50.
Afifi, F. U., Al-Khalidi, B., & Khalil, E. (2005) Studies on the in Vivo Hypoglycemic Activities of Two Medicinal Plants Used in the Treatment of Diabetes in Jordanian Traditional Medicine Following Intranasal Administration. Journal of Ethnopharmacology 100 (3), 314–18. https://doi.org/10.1016/j.jep.2005.03.016
Agyare, C., Dwobeng, A. S., Agyepong, N., Boakye, Y. D., Mensah, K. B., Ayande, P. G., & Adarkwa-Yiadom, M. (2013) Antimicrobial, Antioxidant, and Wound Healing Properties of Kigelia Africana (Lam.) Beneth. and Strophanthus Hispidus DC. Advances in Pharmacological Sciences 2013, 10.
Al-Qudah, T. S., Shibli, R. A., & Alali, F. Q. (2011) “In Vitro Propagation and Secondary Metabolites Production in Wild Germander (Teucrium Polium L.).” In Vitro Cellular and Developmental Biology - Plant 47 (4): 496–505. https://doi.org/10.1007/s11627-011-9352-9
Assimopoulou, A. N., & Papageorgiou, V. P. (2005) “GC-MS Analysis of Penta- and Tetra-Cyclic Triterpenes from Resins of Pistacia Species. Part II. Pistacia Terebinthus Var. Chia.” Biomedical Chromatography 19 (8), 586–605. https://doi.org/10.1002/bmc.484
Baumann, B. B. (1960) The Botanical Aspects of Ancient Egyptian Embalming and Burial Published by : Springer on Behalf of New York Botanical Garden Press Stable. Economic Botany 14 (1), 84–104.
Bozorgi, M., Amin, G., Shekarchi, M., & Rahimi, R. (2017) Traditional Medical Uses of Drimia Species in Terms of Phytochemistry, Pharmacology and Toxicology. Journal of Traditional Chinese Medicine = Chung i Tsa Chih Ying Wen Pan 37 (1), 124–39. https://doi.org/10.1016/s0254-6272(17)30036-5
Buckland, P., & Panagiotakopulu, E. (2001) Rarneses II and the Tobacco Beetle. Antiquity 75 (October 2000), 549–56. http://www.sages.ac.uk/home/homes/rgroves/panagiotakopulupub4.pdf
Buckley, S. A., & Evershed, R. P. (2001) Organic Chemistry of Embalming Agents in Pharaonic and Graeco-Roman Mummies. Nature, no. 413, 837–41.
Clark, K. A., Ikram, S., & Evershed, R. P. (2016) The Significance of Petroleum Bitumen in Ancient Egyptian Mummies. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 374 (2079). https://doi.org/10.1098/rsta.2016.0229
Colombini, M. P., Frezzato, F., Modugno, F., & Ribechini, E. (2007) Caratterizzazione Chimica Dei Balsami Di Mummificazione Delle Mummie e Dei Materiali Pittorici Del Sarcofago Egiziano Conservato Nel Museo Di Anatomia Umana Dell’Università Di Pisa. Egitto e Vicino Oriente 30, 109–16.
Colombini, M. P., Modugno, F., Silvano, F., & Onor, M. (2000) Characterization of the Balm of an Egyptian Mummy from the Seventh Century B.C. Studies in Conservation 45 (1), 19–29. https://doi.org/10.1179/sic.2000.45.1.19
Colombini, M. P., Ribechini E., & Modugno, F. (2004) The Egyptian Mummies in the Museo Di Anatomia of the University of Pisa: A Chemical Approach for the Characterization of Balms. In F. Serra (ed.), Science and Technology for Cultural Heritage, , 1/2 (pp. 83–87). Istituti editoriali e poligrafici internazionali, Pisa. https://doi.org/10.1400/19141
David, R. A. (1992) Plants and Plants Products Used in Mummification. In H. N. Nigg & D. Seigler (Eds.), Phytochemical Resources for Medicine and Agriculture (pp. 15–31). Springer Science+Business Media LLC, New York.
De Pietri, M. (2018). Tra Il Nilo e Il Ticino: La Collezione Egizia Del Museo Archeologico Dell’Università Degli Studi Di Pavia. Gilgames 2 (2), 85–93.
Desroches-Noblecourt, C. (1997) Ramsete II Figlio Del Sole, Sperling & Kupfer, Milan.
De Vartavan, C. T. de. (2007) Pistacia Species in Relation to Their Use as Varnish and ‘Incense’ (Sntr) in Pharaonic Egypt. Bulletin of Parthian and Mixed Oriental Studies 2 (2), 63–92.
Fraser, T. R., & Mackenzie A. T. (1910) Strophanthus Sarmentosus: Its Pharmacological Action and Its Use as an Arrow-Poison. Transactions of the Royal Society of Edinburgh 47 (2), 341–410.
Goyon, J. C., & Vergnieux, R. (1987) Autopsie d’une Momie Egyptienne Du Museum de Lyon In L. David & R. Mourer (Eds.), Nouvelles Archives Du Museum d’Histoire Naturelle de Lyon 25 (pp. 113–15). Musée Guimet d’Histoire Naturelle, Lyon.
Greeff, K., & Schadewaldt, H. (1981) Introduction and Remarks on the History of Cardiac Glycosides. In K. Greeff. (ed.), Handbook of Experimental Pharmacology, (pp 1-12). Springer, Berlin. https://doi.org/https://doi.org/10.1007/978-3-642-68163-9_1
Guimarães, A. C., Meireles, L. M., Lemos, M. F., Guimarães, M. C. C., Endringer, D. C., Fronza, M., & Scherer, R. (2019) Antibacterial Activity of Terpenes and Terpenoids Present in Essential Oils. Molecules 24 (13), 1–12. https://doi.org/10.3390/molecules24132471
Knittel, D. N., Lorenz, P., Huber, U., Stintzing, F. C., & Kammerer, D. R. (2016) Characterization of the Cardiac Glycoside and Lipid Profiles of Strophanthus Kombé Oliv. Seeds. Zeitschrift Fur Naturforschung – Section C Journal of Biosciences 71 (3–4), 55–64. https://doi.org/10.1515/znc-2015-0186
Knittel, D. N., Stintzing, F. C., & Kammerer, D. R. (2015) Metabolic Fate of Cardiac Glycosides and Flavonoids upon Fermentation of Aqueous Sea Squill (Drimia Maritima L.) Extracts. Journal of Pharmaceutical and Biomedical Analysis 110: 100–109. https://doi.org/10.1016/j.jpba.2015.02.050
Łucejko, J., Connan, J., Orsini, S., Ribechini, E., & Modugno, F. (2017) Chemical Analyses of Egyptian Mummification Balms and Organic Residues from Storage Jars Dated from the Old Kingdom to the Copto-Byzantine Period. Journal of Archaeological Science 85, 1–12. https://doi.org/10.1016/j.jas.2017.06.015
Mahboubi, M., Mohammad Taghizadeh Kashani, L., & Mahboubi M. (2019) Squill (Drimia Maritima L.) and Its Novel Biological Activity. Oriental Pharmacy and Experimental Medicine 19 (3), 227–34. https://doi.org/10.1007/s13596-018-0341-y
Malgora, S. (2013) Il caso di Ankhpakhered. I nuovi approcci scientifici del Mummy Project. Ananke, Turin.
Malgora, S. (2014) Un corpo per l’eternità. In B. Cerasetti, & K. Lamberg-Karlovsky (eds.), My Life like the summer rose. Maurizio Tosi e l’archeologia come modo di vita (pp.180-194). British Archaeological Report, Oxford.
Malgora, S., & Elias, J. (2014) The Mummy Project. The case of Ankhpakhered: CT scan analysis. In H. Gill-Frerking, A. Zink, & W. Rosendahl (eds), Yearbook of Mummy Studies, volume II.
Malgora, S., Zink, A., Milani, C., Elias, J., & Rosendahl, W. (2017) La Mummia di Merano, Palais Mamming, Meran.
Ménager, M., Azémard C., & Vieillescazes, C. (2014) Study of Egyptian Mummification Balms by FT-IR Spectroscopy and GC-MS. Microchemical Journal 114: 32–41. https://doi.org/10.1016/j.microc.2013.11.018
Mezzatesta, E., Perraud, A., & Vieillescazes, C. (2020) Analysis of Balms Taken from Egyptian Human Mummies Using Solid-Phase Extraction and Gas Chromatography – Mass Spectrometry. Journal of Separation Science, no. December, 1–10. https://doi.org/https://doi.org/10.1002/jssc.202000746
Moser, S., & Nicola, G. L. (2017) Sharing Knowledge for Restoring Coffins: The Case of Civico Museo Di Storia e Arte of Trieste. In A. Amenta & H. Guichard, (Eds.), Proceedings of the First Vatican Coffin Conference 19-22 June 2013, (pp. 317–26). Edizioni Musei Vaticani, Vatican
Nicola, G. L., Nicola, M., & Nicola, A. (2008) Preservation and Conservation of Mummies and Sarcophagi. E-Conservation Magazine 3 (3), 22–47.
Ofori–Baah, S. & Borquaye, L. S. (2019) Ethanolic Leaf Extract from Strophanthus Gratus (Hook.) Franch. (Apocynaceae) Exhibits Anti-Inflammatory and Antioxidant Activities. Cogent Biology 5 (1), 1710431. https://doi.org/10.1080/23312025.2019.1710431
Régert, M., Colinart, S., Degrand, L., & Decavallas, O. (2001) Chemical Alteration and Use of Beeswax Through Time: Accelerated Ageing Tests and Analysis of Archaeological Samples from Various Environmental Contexts. Archaeometry., no. 43, 549–69.
Sadeghi, H., Jamalpoor, S., & Shirzadi, M. H. (2014) Variability in Essential Oil of Teucrium Polium L. of Different Latitudinal Populations. Industrial Crops and Products 54, 130–34. https://doi.org/10.1016/j.indcrop.2014.01.015
Sebedio, J. L., Le Quere, J. L., Semon, E., Morin, O., Prevost, J., & Grandgirard, A. (1987) Heat Treatment of Vegetable Oils. II. GC-MS and GC-FTIR Spectra of Some Isolated Cyclic Fatty Acid Monomers. Journal of the American Oil Chemists’ Society 64 (9), 1324–33. https://doi.org/10.1007/BF02540791
Tchapla, A., Méjanelle, P., Bleton, J., & Goursaud, S. (2004) Characterisation of Embalming Materials of a Mummy of the Ptolemaic Era. Comparison with Balms from Mummies of Different Eras. Journal of Separation Science 27 (3), 217–34 https://doi.org/10.1002/jssc.200301607