The relationship of polymorphism with explosive forces in ACTN3, ACE, and UCP3 genes in soccer players

Main Article Content

Engin Gunes Atabas
Ayşegül Yapıcı Öksüzoğlu
Samet Turel
Hakan Akca

Keywords

ACTN3, ACE, UCP3, Genotype, Polymorphism, Soccer

Abstract

Study Objectives: The purpose of the study was to investigate the relationship of polymorphism with explosive forces in ACTN3, ACE, and UCP3 genes in soccer players. Methods: A total of 19 male soccer players and 9 sedentary voluntarily participated. Countermovement jump, squat jump, and standing long jump tests were applied to the subjects. On the same day, the subjects performed the jump tests twice again and the best score was recorded. Blood was drawn from subjects for Genomic DNA isolation. PCR products of ACTN3 and UCP3 genes Medical Genetics department genotypic differences between the groups were determined by analyzing with an automatic DNA sequencing system. Since taken non-parametric assumptions, it was analyzed by the Spearman test. Results: In subject group, a statistically significant relationship was found between all three jump performances in the subjects with ACTN3 RR and ACE ID genotype (p CMJ=0.04; SJ=0.04; SLJ=0.05) (p<0.05). In subject group, statistically significant correlation was found between countermovement jump and squat jump performances in subjects with ACTN3 XX and UCP3 -55C/T genotype (p CMJ=0.04; SJ= 0.04) (p<0.05), the same relationship was not found in the control group with the genotype ACTN3 XX and UCP3 -55C/T (p CMJ=0.1; SJ=0.11; SLJ=0.1) (p>0.05). A statistically significant relationship was found between all three jump performances in subjects with ACE ID and UCP3 -55C/C genotype (p CMJ=0.00; SJ=0.00; SLJ=0.00) (p<0.05). Conclusion: We found that, associations of explosive forces with polymorphisms. Although genotypes of athletes are similar to previous studies, stronger evidence is needed to establish a meaningful relationship between genotype and explosive power.

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References

1. Sessa F, Chetta M, Petito A, et al. Gene Polymorphisms and Sport Attitude in Italian Athletes. Genet Test and Mol Bioma, 2011;15 (4), 285-290.
2. Ulucan K, Sercan C, Biyikli T. Distribution of Angiotensin-1 Converting Enzyme Insertion/Deletion and α-Actinin-3 Codon 577 Polymorphisms in Turkish Male Soccer Players. Genet Epigenet, 2015;20(7),1-4.
3. Ortega S, Marión RM, Strati K, et al. A p53 mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity. Nature, 2009; 460(7259), 1149.
4. Ahmetov II, Wang G, Mikami E, et al. Association analysis of ACE and ACTN3 in elite Caucasian and East Asian swimmers. Med Sci Sports Exerc, 2013; 45:892-900.
5. Lopez-Leon S, Janssens ACJW, Ladd AGZ, et al. Meta-analyses of genetic studies on major depressive disorder. Mol Psychiatry, 2008;13(8), 772.
6. Bayyurt GM, Ulucan K, Konuk M, et al. Effect of alpha-actinin-3 gene on Turkish trained and untrained middle school children’s sprinting performance: a pilot study. Biol Rhythm Res, 2014; 45(4): 509-14.
7. Bouchard C, Dionne F, Simoneau F, et al. Genetics of aerobic and an-aerobic performances. Exerc. Sport Sci Rev, 1992; 20:27-58.
8. Yang N, MacArthur DG, Gulbin JP, et al. ACTN3 genotype is associated with human elite athletic performance. Am J Hum Genet, 2003; 73(3):627-31.
9. Montgomery HE, Marshall R, Hemingway H, et al. Humangene for physical performance. Nature, 1998; 393(6682): 221-2.
10. McKanna TA, Toriello HV. Gene doping: The hype and the harm. Pediatr Clin N Am, 2010; 57:719-27. http://dx.doi.org/10.1016/j.pcl.2010.02.006.
11. Goldstein DB, & Cavalleri GL. Genomics: understanding human diversity. Nature, 2005; 437(7063), 1241.
12. Ma F, Yang Y, Li X, et al. The association of sport performance with ACE and ACTN3 genetic polymorphisms: a systematic review and meta-analysis. PLoS One, 2013;8: e54685.
13. Coates D. The angiotensin converting enzyme (ACE). Int J Biochem Cell Biol, 2003; 35: 769-773.
13. MacArthur DG, North KN. A gene for speed? The evolution and function of alpha actinin-3. Bio Essays, 2004; Jul;26(7):786-95. http://dx.doi.org/10.1002/bies.20061.
14. Ahmetov II, Druzhevskaya AM, Astratenkova IV, et al. The ACTN3 R577X polymorphism in Russian endurance athletes. Br J Sports Med, 2010; 44:649-52.
15. Ahmetov, II, Druzhevskaya AM, Lyubaeva EV, et al. The dependence of preferred competitive racing distance on muscle fibre type composition and ACTN3 genotype in speed skaters. Exp Physiol, 2011; 96(12):1302–10.
16. Echegaray M, Rivera I, Wolfarth B, et al. Uncoupling protein 3 gene polymorphism and elite endurance athlete status: the geneathlete study. Med Sci Sports Exerc, 2003; 35:S378.
17. Fang QC, Jia WP, Yang M, et al. Effect of polymorphism of uncoupling protein 3 gene-55 (C>T) on the resting Energy expenditure, total body fat and regional body fat in Chinese. Zhonghua yi xue yi chuan xue za zhi= Zhonghua yixue yichuanxue zazhi= Chin J Med Genet, 2005; 22(5), 485-488.
18. Schrauwen P, Xia J, Walder K, et al. A novel polymorphism in the proximal UCP3 promoter region: effect on skeletal muscle UCP3 mRNA expression and obesity in male non diabetic Pima Indians. Int J Obes Relat Metab Disord, 1999; 23: 1242–1245.
19. Castellano D, Mackay TF, Richards S, et al. The Drosophila melanogaster genetic reference panel. Nature, 2012; 482(7384), 173.
20. Stolen T, Chamari K, Castagna C, et al. Physiology of soccer: an update. SportsMed, 2012; 35(6):501–536.
21. Greig MP, Mcnaughton LR, Lovell RJ. Physiological and mechanical response to soccer speciWc intermittent activity and steady-state activity. Res Sports Med, 2014; 14: 29-52.
22. Sporis G, Jukic I, Milanovic L, et al. Reliability and factorial validity of agility tests for soccer players. J Strength Cond Res, 2010; 24(3): 679–686.
23. Çorak, A, Kapıcı S, Sercan C, et al. A pilot study for determination of anxietyrelated SLC6A4 promoter "S" and "L" alleles in healthy Turkish athletes. Mol Cell Biol, 2017; 63(5): 29-31.
24. Egorova ES, Borisova AV, Mustafina LJ, et al. The polygenic profile of Russian football players. J Sports Sci. 2014; 32(13): 1286–1293.
25. Pimenta EM, Coelho DB, Cruz IR. The ACTN3 genotype in soccer players in response to acute eccentric training. Eur J Appl Physiol. 2012; 112(4): 1495–1503.
25. Santiago C, Gonzalez-Freire M, Serratosa L. ACTN3 genotype in professional soccer players. Br J Sports Med. 2008; 42(1):71–73.
26. Eynon N, Hanson ED, Lucia A, et al. Genes for Elite Power and Sprint Performance: ACTN3 Leads the Way, Sports Med 2013; 43: 803–817.
27. Papadimitriou ID, Lucia A, Pitsiladis YP, et al. ACTN3 R577X and ACE I/D gene variants influence performance in elite sprinters: a multi-cohort study. BMC Genomics, 2016; 17: 285.
28. Kim H, Song K, Kim C. The ACTN3 R577X variant in sprint and strength performance. J Exerc Nutr Biochem, 2014; 18: 347–353.
29. Ma F, Yang Y, Li X, et al. (2013) The Association of Sport Performance with ACE and ACTN3 Genetic Polymorphisms: A Systematic Review and MetaAnalysis. PLoS One 8(1): e54685.
30. Gineviciene V, Jakaitiene A, Aksenov MO. Association analysis of ACE, ACTN3 and PPARGC1A gene polymorphisms in two cohorts of European strength and power athletes. Biol Sport. 2016; 33(3): 199–206.
31. Chih LL, Ryan MR, Paul DH, et al. Histone Variant H2A.Z Marks the 5’ Ends of both Active and Inactive Genes in Euchromatin. Cell, 2005; 123: 233-248.
32. Juffer P, Furrer R, González-Freire M, et al. Genotype Distributions in Top-level Soccer Players: A Role for ACE? Int J Sports Med, 2009; 30(05): 387–392.