Main Article Content
Fruit, vegetable, fresh produce, E. coli, market, contamination
This study explores the effect of human handling on E. coli contamination of fresh produce (FP) in the overlooked market stage where customers touch FP. Study includes an observational and a comparative part with 3 experimental sample groups as; (i) Control group (CG), (ii) Touched FP group (TG) and (iii) FP touched with gloves group (GG). In the comparative part, generic E. coli and STEC were screened, quantified and analyzed for antibiotic susceptibilities. The average score of sellers’ knowledge level was low (39%). E. coli counts from banana, lettuce, carrot and tomato were found to be significantly higher (p=0.037, p=0.046, p=0.046 and p=0.034 respectively) in TG compared to CG which indicates human handling as a significant factor of bacterial contamination in the market. E. coli counts were significantly lower for banana, lettuce and tomato in GG (p=0.037, p=0.001, p=0.034 respectively) which is probative for the dissemination at the market through human handling and is also indicating this as a useful/practical prevention tool. Shiga toxin-producing E. coli (STEC-O157:H7) was isolated only from one lettuce sample in TG. The highest resistance rate was observed for ampicillin (44%), followed by cephalothin (40%), tetracycline (24%) and amoxicillin-clavulanic (24%). Here, we demonstrate that human handling of FP in the market stage is a significant factor on E. coli contamination which may include STEC. Furthermore, our findings suggest that, this contamination is preventable by using gloves or similar practical materials.
2. Hussain M, Gooneratne R. Understanding the Fresh Produce Safety Challenges. Foods. 2017;6(3):23.
3. World Health Organization. WHO estimates of the global burden of foodborne diseases: foodborne disease burden epidemiology reference group 2007-2015. World Health Organization; 2015.
4. Warriner K, Huber A, Namvar A, Fan W, Dunfield K. Recent advances in the microbial safety of fresh fruits and vegetables. Adv Food Nutr Res. 2009;57:155–208.
5. Centers for disease control and prevention. Multistate outbreak of listeriosis linked to packaged salads produced at Springfield, Ohio Dole processing facility (final update). Centers Dis Control Prev Atlanta, GA http//www cdc gov/listeria/outbreaks/bagged-salads-01-16/index html Accessed August. 2016;
6. Olaimat AN, Holley RA. Factors influencing the microbial safety of fresh produce: A review. Food Microbiol [Internet]. 2012;32(1):1–19. Available from: http://dx.doi.org/10.1016/j.fm.2012.04.016
7. Mritunjay SK, Kumar V. A study on prevalence of microbial contamination on the surface of raw salad vegetables. 3 Biotech. 2017;7(1):1–9.
8. Food and Drug Administration. Guidance for industry: Guide to minimize microbial food safety hazards for fresh fruits and vegetables. Fed Regist Oct [Internet]. 2008;20204(FDA-2008-D-0108):49. Available from: http://scholar.google.com/scholar?hl=en&btnG=Search&q=intitle:Guidance+for+Industry+Guide+to+Minimize+Microbial+Food+Safety+Hazards+for+Fresh+Fruits+and+Vegetables#1%5Cnhttp://scholar.google.com/scholar?hl=en&btnG=Search&q=intitle:Guidance+for+industry:+G
9. Van Pelt AE, Quiñones B, Lofgren HL, Bartz FE, Newman KL, Leon JS. Low Prevalence of Human Pathogens on Fresh Produce on Farms and in Packing Facilities: A Systematic Review. Front Public Heal. 2018;6(February):1–10.
10. I S O (International Organization for Standardization). ISO 16654/2001. Microbiology of food and animal feeding stuffs—Horizontal method for the detection of Escherichia coli O157. 2001;
11. EUCAST. European Committee on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters European Committee on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters. Eur Comm Antimicrob Susceptibility Testing Break tables Interpret MICs Zo diameters Version 80, 2018 http//www.eucast.org [Internet]. 2015;0–77. Available from: http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_8.0_Breakpoint_Tables.pdf
12. Cardamone C, Aleo A, Mammina C, Oliveri G, Di Noto AM. Assessment of the microbiological quality of fresh produce on sale in Sicily, Italy: preliminary results. J Biol Res. 2015;22(1):1–6.
13. Seow J, ágoston R, Phua L, Yuk HG. Microbiological quality of fresh vegetables and fruits sold in Singapore. Food Control. 2012;25(1):39–44.
14. Denis N, Zhang H, Leroux A, Trudel R, Bietlot H. Prevalence and trends of bacterial contamination in fresh fruits and vegetables sold at retail in Canada. Food Control. 2016;67:225–34.
15. Pagadala S, Marine SC, Micallef SA, Wang F, Pahl DM, Melendez M V., et al. Assessment of region, farming system, irrigation source and sampling time as food safety risk factors for tomatoes. Int J Food Microbiol [Internet]. 2015;196:98–108. Available from: http://dx.doi.org/10.1016/j.ijfoodmicro.2014.12.005
16. Dharmarha V, Pulido N, Boyer RR, Pruden A, Strawn LK, Ponder MA. Effect of post-harvest interventions on surficial carrot bacterial community dynamics, pathogen survival, and antibiotic resistance. Int J Food Microbiol [Internet]. 2019;291(October 2018):25–34. Available from: https://doi.org/10.1016/j.ijfoodmicro.2018.11.006
17. EUROPEIA C. Commission regulation (EC No 2073/2005 of 15 November 2005 on microbial criteria for foodstuffs. Off J Eur Union. 2005;L338:1–26.
18. Al-Kharousi ZS, Guizani N, Al-Sadi AM, Al-Bulushi IM. Antibiotic Resistance of Enterobacteriaceae Isolated from Fresh Fruits and Vegetables and Characterization of their AmpC beta-Lactamases. J Food Prot. 2019 Nov;82(11):1857–63.
19. Fairbrother JM, Vounba P, Arsenault J, Bada-alambe R. Prevalence of antimicrobial resistance and potential pathogenicity , and possible spread of third generation cephalosporin resistance , in Escherichia coli isolated from healthy chicken farms in the region of Dakar , Senegal. 2019;1–23.
20. Schwaiger K, Helmke K, Hölzel CS, Bauer J. Antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket). Int J Food Microbiol. 2011;148(3):191–6.