Production of linoleic acid metabolites by different probiotic strains of Lactobacillus plantarum

Main Article Content

Tariq Aziz
Abid Sarwar
Sam Al Dalali
Zia Ud Din
Sarah Megrous
Jalal Ud Din
Xin Zou
Yang Zhennai

Keywords

Lactobacillus Plantarum, Linoelic acid metabolites, GC MS

Abstract

Objective: To evaluate the capability of Lactobacillus plantarum to grow and convert polyunsaturated free fatty acids, i.e. linoleic acid (LA) into bioactive and less toxic conjugated fatty acids.  Method: Six L. plantarum strains were grown in MRS medium containing LA from 1% to 10% (w/v), and the LA metabolites formed in the medium were identified and quantitated by GC-MS. Result: In respect to the identified LA metabolites, a total of 4 metabolites were detected including linoelaidic acid, 9,12-octadecadienoic acid (Z, Z), 9,12-Octadecadienoic acid, methyl ester, and trans,trans-9,12-octadecadienoic acid, propyl ester. Among all the six L. plantarum strains, the best one was L. plantarum 2-3 which showed maximum growth and conversion of linoleic acid to different metabolites from 1% to 10 % (w/v) of LA supplied, while L. plantarum 12-5 showed maximum growth and conversion at higher concentration of LA supplied and showed minimal growth and conversion at lower concentration of LA. Conclusion: Among all the six L. plantarum strains, the best one was L. plantarum 2-3 which showed maximum growth and conversion of LA to different metabolites.

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