Determination and evaluation of the pyridoxal, pyridoxine, and pyridoxamine forms of vitamin B6 in plant-based foods in terms of healthy vegetarian nutrition Vitamin B6 profile in plant baded foods

Main Article Content

Sabiha Zeynep Aydenk Koseoglu

Keywords

Vitamin B6, pyridoxal, pyridoxine, pyridoxamine, vegetarian nutrition, plant-based foods, HPLC.

Abstract

Background and Objective: Vitamin B6 deficiency is observed in vegetarians. In many studies, vitamin B6 is determined as the sum of the pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) forms. Because the bioavailability of the PL, PN and PM forms of vitamin B6 are different, knowing the amounts of these forms in foods is important for healthy nutrition. The aim of this study was to determine the PL, PN and PM forms of vitamin B6 in plant-derived foods and to evaluate these forms in terms of vegetarian and nonvegetarian nutrition. Methodology: In this study, a total of 61 foods from the vegetable (26), fruit (19), grain (10), legume (3) and nut (3) categories were examined using high-performance liquid chromatography (HPLC). Results: It was found that vegetables, fruits and grains contained more of the PN form along with the PL and PM forms, while the PL form was found in high levels in nuts. The PL form was found at an average rate of 29.6% in vegetables and 19.4% in fruits. The PN form was found predominantly in legumes and grains. Conclusions: When we evaluated the results, vegetables, fruits and grains contained more of the PN form along with the PL and PM forms, while the PL form was found in high amounts in nuts. Many vegetables contain low amounts of protein, but they contain high amounts of vitamin B6 in the PLP form. These foods can be consumed together with foods that contain high levels of protein (legumes, grains) and low levels of vitamin B6 in the context of vegetarian nutrition. This profile determination study will be an important source of information for the planning of various healthy diets for vegetarians as well as nonvegetarians.

Abstract 1549 | PDF Downloads 2085

References

1. Phillips F. Vegetarian nutrition. Nutr Bull 2005; 30:132–167.
2. Vudhivai N, Ali A, Pongpaew P, Changbumrung S, Vorasanta S, Kwanbujan K, Charoenlarp P, Migasena P, Schelp FP. Vitamin B1, B2 and B6 status of vegetarians. J Medical Assoc Thailand 1991; 74:465-470.
3. Huang YC, Chang SJ, Chiu YT, Chang HH, Cheng CH.. The status of plasma homocysteine and related B-vitamins in healthy young vegetarians and nonvegetarians. European J Nutr 2003; 42:84–90.
4. Majchrzak D, I Singer, Männer M, Rust P, Genser D, Wagner KH, Elmadfa I. B-Vitamin Status and Concentrations of Homocysteine in Austrian Omnivores, Vegetarians and Vegans. AnnnNutr Metabolism 2006; 50:485–491.
5. Eittenmiller RR, Lin YWO, Landen Jr. Vitamin B6. Vitamin Analysis for Food the Health and Food Sciences, Second Edition, CRC Press, New York, 2008, pp. 471-488.
6. Ball GFM. Vitamin B6. Vitamins: Their Role in the Human Body, First Edition, Blackwell Publishing Ltd, Oxford, UK, 2004, pp. 310-325.
7. Miller JW., Ribaya-Mercado JD, Russell RM. Shepard DC, Morrow FD , Cochary EF, Sadowski JA, Gershoff SN, J Selhub. Effect of vitamin B-6 deficiency on fasting plasma homocysteine concentrations. Am J Clinic Nutr.1992; 55:1154–60.
8. Miller LT, Leklem JE, Shultz TD. The effect of dietary protein on the metabolism of vitamin B-6 in humans. J Nutr 1985; 115:1663–1672.
9. Hansen, CM, Leklem JE, Miller LT. Vitamin B-6 status of women with a constant intake of vitamin B-6 changes with three levels of dietary protein. J Nutr 1996; 126:1891–901.
10. Food and Nutrition Board. Vitamin B6: Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline, National Academy Press, Washington, DC., 1998, pp.150–195.
11. United States Department of Agriculture. USDA Food Composition Databases. Available at: https://ndb.nal.usda.gov/ndb/, Accessed April, 21, 2018
12. Gregory JFIII, Trumbo PR, Bailey LB, Toth JP, Baumgartner TG, Cerda J. Bioavailability of pyridoxine-5´-β-D glucoside determined in humans by stable-isotopic methods. J Nutr 1991; 121:177–186.
13. Rossouw JE, Labadarios D, Davis M, R. Williams. Vitamin B6 and aspartate aminotransferase activity in chronic liver disease. South Afric Medical J 1978; 53:436-438.
14. Mann DL, Ware GM, Bonnin E, Eitenmiller RR. Liquid Chromatographic Analysis of Vitamin B6 in Reconstituted Infant Formula: Collaborative Study. J AOAC Int 2001; 88:30-37.
15. Kall M. A. Determination of total vitamin B6 in foods by isocratic HPLC: a comparison with microbiological analysis. Food Chem 2003; 82:315-327.
16. Ceylan Z, Yaman M, Sağdıç O, Karabulut E, Yilmaz MT.. Effect of electrospun thymol-loaded nanofiber coating on vitamin B profile of gilthead sea bream fillets (Sparus aurata). LWT 2018; 98:162-169.
17. Gregory JFIII, Ink SL. Identification and quantification of pyridoxine-β-glucoside as a major form of vitamin B6 in plant-derived foods. J Agric Food Chem 1987; 35:76–82.
18. Sampson DA, Eoff LA, Yan XL, Lorenz K.. Analysis of Free and Glycosylated Vitamin B6 in Wheat by High-Performance Liquid Chromatography. Cereal Chem 1995; 72: 217-221.
19. Sauberlich HE. Bioavailability of vitamins. Progress Food Nutr Science 1985; 9:1–33.
20. Reynolds RD. Bioavailability of vitamin B-6 from plant foods. Am J Clinic Nutr 1988; 48:863–867.
21. Su-Yen G, Mark EC. The Role of Advanced Glycation End Products in Progression and Complications of Diabetes. J Clinic Endocr Metabolism 2008; 93:1143–1152.