Cell culture developing and the imaging of total protein product changing with SDS-PAGE in Saccharomyces cerevisiae
Keywords:
S. cerevisiae, mulberry juice, hidrogen peroxide, protein expression, SDS-PAGEAbstract
Five groups were created in this work. i: Control group, ii: H2O2 group, iii: H2O2 + %10 Mulberry Juice (MBJ) group, iv: H2O2 + %15 MBJ, v: H2O2 + %25 MBJ group. After sterilization, H2O2 and fruit juice were inserted different concentration to Saccharomyces cerevisiae (S. cerevisiae) cultures and the cultures were developed at 30°C for 1h, 3h, 5h and 24 hours (overnight). S. cerevisiae cell growth was computed by spectrophotometer, total protein alteration was analysed by SDS-PAGE electrophoresis and reckoned with bradford method. our studies results indicated that; cell developing increased in MBJ groups in proportion to the positive control (H2O2) group at different growing times (1, 3, 5 and 24 hours) (p<0,05). As a result MBJ has a preservative role for reduce the oxidative damage and expanded cell developing and encourage protein synthesis in S. cerevisiae.
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