Extraction, purification and macrophage stimulatory activity of polysaccharide isolated from leaves of Perilla frutescens BRITTON var. crispa

Yu Jeong Kim; Jung Soon Hwang; Ki Han Kwon

doi:10.23751/pn.v21i1-S.5525

Extraction, purification and macrophage stimulatory activity of polysaccharide isolated from leaves of Perilla frutescens BRITTON var. crispa

Authors

Yu Jeong Kim Department of Nursing, Chosun Nursing College, Gwangju 61453, South Korea
Jung Soon Hwang Division of Beauty Care Arts, Department of Practical Arts, Graduate School of Culture and Arts, Dongguk University, Seoul 04620, South Korea
Ki Han Kwon Department of Food Science and Nutrition, College of Health, Welfare and Education, Gwangju University, Gwangju 61743, South Korea

Keywords:

immunostimulatory activity, macrophage, Perilla frutescens BRITTON var. crispa, polysaccharide, purification

Abstract

The crude polysaccharide portion of Perilla frutescens BRITTON var. crispa (PFB-1) was prepared by following steps, hot water extraction (PFB-0), decolorization with methanol reflux and ethanol precipitation. The PFB-1 was separated by anion-exchange column chromatography (fraction: PFB-1-0). Purified PFB-1-0-ii was obtained from gel permeation column chromatography purification steps of PFB-1-0. The PFB-1-0-ii was shown to be one polysaccharide with molecular mass of 10 kDa (kilodalton). PFB-1-0-ii consists of mannose, galactose and glucose as major sugars with 82.8% carbohydrate. Next, this study investigated the effects of PFB-0, PFB-1, PFB-1-0, and PFB-1-0-ii on macrophage stimulatory activity in primary macrophages. PFB-0, PFB-1, PFB-1-0 and PFB-1-0-ii increased macrophage cellular lysosomal enzyme activities. Among the samples tested, PFB-1-0-ii exhibited the highest macrophage lysosomal enzyme activities. The macrophage-stimulating activity was significantly enhanced during the purification step. The results support the utility of purified polysaccharide from Perilla as immunostimulatory activity agent.

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Published

06-05-2019

Issue

Vol. 21 No. 1-S (2019): Supplement 1/2019: Community nutrition from childhood to old age

Section

Original articles

License

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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How to Cite

Kim YJ, Hwang JS, Kwon KH. Extraction, purification and macrophage stimulatory activity of polysaccharide isolated from leaves of Perilla frutescens BRITTON var. crispa. Progr Nutr [Internet]. 2019 May 6 [cited 2025 Mar. 4];21(1-S):399-405. Available from: https://mattioli1885journals.com/index.php/progressinnutrition/article/view/5525