Shofiul Azam
Department of Pharmaceutical Sciences, North South University, Block-B, Bashundhara R/A, Dhaka-1229, Bangladesh.
Prawej Ansari
Department of Pharmaceutical Sciences, North South University, Block-B, Bashundhara R/A, Dhaka-1229, Bangladesh;
Department of Pharmacy, International Islamic University Chittagong, 154/A, college road,
Chittagong-4203, Bangladesh
Shahnaz Jalil
Department of Pharmaceutical Sciences, North South University, Block-B, Bashundhara R/A, Dhaka-1229, Bangladesh
Abdunasir Hussain Ibrahim
Department of Pharmaceutical Sciences, North South University, Block-B, Bashundhara R/A, Dhaka-1229, Bangladesh
Nasrin Sultana
Department of Pharmaceutical Sciences, North South University, Block-B, Bashundhara R/A, Dhaka-1229, Bangladesh
Mohammad Mosharraf Hossain
Department of Pharmacy, University of Science and Technology Chittagong (USTC), Bangladesh
Jaysee Muhammad Naveed
Department of Pharmacy, Southeast University, 24 Kamal Ataturk Avenue, Dhaka-1213, Bangladesh
Mohammed Forhad Hossain
Department of Pharmacy, International Islamic University Chittagong, 154/A, college road,
Chittagong-4203, Bangladesh
Keywords
analgesic, anti-inflammatory, C. speciosus, HRBC, inflammation, protein denaturation
Abstract
Goal: This study was designed to investigate the anti-inflammatory and analgesic activity of one of common Bangladeshi medicinal plant, Costus speciosus. Methods & materials: We have included the phytochemical investigation before proceeding to our major objective. The seeds of Costus speciosus (MECS) were extracted using methanol. MECS have been used in in vitro and in vivo investigation for possible analgesic and anti-inflammatory effect. In this pre-designed study Formalin-induced writhing test and Tail immersion test was included. Results: The methanol extract of C. speciosus (at 500 mg/kg) showed significant (P<0.01) anti-inflammatory activity (51.09±1.67% and 75.93±2.58% shown by standard) on red blood cell membrane stabilization study, and the crude extract have included 75.15±2.33% (p<0.05) of protection against protein denaturation. In tail immersion study the extract at the dose of 500 mg/kg increases the response time up to 5.97±0.11 second, which was close enough to reference group (8.51±0.21 second) at 90 min of drug administration. C. speciosus relapse the writhing, up to 53.97% during first phase of inflammation induced by formalin, it also reduced pain substantially (59.89%; p<0.05) at second phase of study, where standard group showed 73.78% pain reduction. Conclusion: This study presents a very good potentiality in inflammatory mediators inhibition. From the result of our study, it is clear that it possess analgesic and anti-inflammatory activity. Further study is suggested for active compound isolation and adjustment.
Abstract 
