Identification of bioactive compounds and determination of totalphenolic and flavonoid contents in leaf extracts originated from the Algerian desert Olea europaea subsp. laperrinei and Olea europaea subsp. europaea var. Sylvestris and evaluation of their potential as antioxidants
Main Article Content
Keywords
Olea europaea L, subsp. laperrinei, var. sylvestris, phytochemical profile, total phenolic, flavonoids contents, antioxidant potential
Abstract
Since ancient times, Olea europaea L. Notably, the olive leaf has been used in ethnopharmacology to treat fevers and malaria. Currently, this aerial part aroused the interest of researchers around the world in the fields of medicine and pharmacology due to their beneficial effects on human health, including anti-hypertensive hypoglycemic, hypocholesterol, antimicrobial properties, as well as utilized to prevent Alzheimer's disease and to provide protection from colon, breast, and ovarian cancers. Additionally,has a great antioxidant potential due to their high phenolic content.Despite the knowledge of bioactivities of olives that have been frequently reported, the majority of them were related to cultivated olives, whereas wild olives are even less recognized or unknown like our case subsp. laperrinei. The purpose of this work was to realize a comparative evaluation of the phytochemical profile, total phenolic, and flavonoids contents, as well as the antioxidant potential of both aqueous and methanolic extract from two subspecies such as Olea europea subsp.laperrinei from Sahara and Olea europaea var. sylvestris from north of Algeria has been done. In order to understand how environmental stress exerted on these wild species affects the capacity to synthesizesecondarymetabolites as well as antioxidant potential. HPLC analysis showed that Oleuropein is the main compound in all extracts in which the Saharan extracts showed a height level (276.157 mg/g), furthermore, all the extracts obtained showed reasonably high total phenolic and flavonoid contents and good radical scavenging activity notably those from subsp.laperrinei were more important. Furthermore, the highest values were obtained using methanol as solvents than water. The results also showed that wild olives have very high antioxidant potentials andit could be deduced that their leaves can under stress conditions increase the synthesis of bioactive as in the case of subsp. laperrinei compared to var. sylvestris from no stressful condition.
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22. Olmo-García L, Bajoub A, Benlamaalam, S, et al. Establishing the phenolic composition of Olea europaea L. leaves from cultivars grown in Morocco as a crucial step towards their subsequent exploitation. Molecules. 2018; 23: 2524. doi:10.3390/molecules23102524.
23. Nicolì F, Negro C, Vergine M, et al. Evaluation of phytochemical and antioxidant properties of 15 Italian Olea europaea L. cultivar leaves. Molecules. 2019;24, 1998. doi:10.3390/molecules24101998 .
24. Abaza L, Taamalli A, Nsir H, ZarroukM. Olive Tree (Olea europeae L.) Leaves: Importance and Advances in the Analysis of PhenolicCompounds.Antioxidants. 2015; 4: 682-698; doi:10.3390/antiox4040682 .
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30. Nadhala R A, Moyo M, Staden VJ. Natural Antioxidants: Fascinating or Mythical Biomolecules. Molecules. 2010; 15:6905-6930.doi: 10.3390/molecules15106905.
31. Azwanida N N. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation.MedAromat Plants. 2015; 4: 196. doi: 10.4172/2167-0412.1000196.
32. Bouaziz M, Fki I, Jemai H, Ayadi M, Sayadi S. Effect of storage on refined and husk olive oils composition: Stabilization by addition of natural antioxidants from Chemlali olive leaves. Food Chem.2008 ;10: 253–262.doi: 10.1016/j.foodchem.2007.10.074.
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34. Handa S, Khanuja S P, Longo GRakesh D D.Extraction Technologies for Medicinal and Aromatic Plants. UNIDO and ICS , Trieste, Italy .2008; 260 : 21–54.
35. Vongsaka B, Sithisarna P, Mangmool S, Thongpraditchotec S, Wongkrajangc Y, Gritsanapana W. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind Crops Prod. 2013;44: 566–571. doi: 10.1016/j.indcrop.2012.09.021.
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Bouchoucha S, Boukhebti H, Belhadj H, Oulmi A, Chaker AN. Identification of bioactive compounds and determination of totalphenolic and flavonoid contents in leaf extracts originated from the Algerian desert Olea europaea subsp. laperrinei and Olea europaea subsp. europaea var. Sylvestris and evaluation of their potential as antioxidants. Progr Nutr [Internet]. 2023 Sep. 27 [cited 2024 Nov. 27];25(3):e2023032. Available from: https://mattioli1885journals.com/index.php/progressinnutrition/article/view/14253
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Bouchoucha S, Boukhebti H, Belhadj H, Oulmi A, Chaker AN. Identification of bioactive compounds and determination of totalphenolic and flavonoid contents in leaf extracts originated from the Algerian desert Olea europaea subsp. laperrinei and Olea europaea subsp. europaea var. Sylvestris and evaluation of their potential as antioxidants. Progr Nutr [Internet]. 2023 Sep. 27 [cited 2024 Nov. 27];25(3):e2023032. Available from: https://mattioli1885journals.com/index.php/progressinnutrition/article/view/14253
