Study on the chemical composition, antioxidant and antimicrobial activity of essential oils obtained from leaves of Tunisian Anethum graveolens L.

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Marwa Khammassi
Mouna Souihi
Oumayma Kochti
Sophia Loupasaki
Ismail Amri
Bassem Jamoussi
Abdelhamid Khaldi


essential oil, Apiaceae, biological activity


Anethum graveolens L. is a very widespread aromatic and medicinal plant which is widely used as a spice for culinary preparations and has been used for several application that medicine and industry. Most of phytochemical and biological activity studies have focused on molecules produced by A. graveolens seeds and little reports were carried out particularly on essential oils (EOs) produced by leaves. Indeed, to our knowledge, no reports conducted on the essential oils produced by Tunisian A. graveolens. In the current study, EOs of A. graveolens leaves collected from two different origins were extracted by hydrodistillation. Then, EOs were identified by using gas chromatography and mass spectrometry. In addition, the antimicrobial activity of EOs were evaluated against six pathogenic bacteria: Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus and tow fungus strain: Candida albicans and Aspergilus niger. The antioxidant potential of tested EOs was evaluated by four different tests: Total antioxidant activity (TAA), DPPH, ABTS and reducing power assay (RPA). GC analysis indicated the presence of twelve compounds that the most predominant compounds were p-cymene (41.51-62.07%), followed by limonene (27-28%) and α-phellandrene (1.43-21.45%). The two oils showed differences related to their provenance. both oils have shown antioxidant potential which reflects their ability to scavenge free radicals. Likewise, very important and remarkable antimicrobial activities have been observed. All these properties make the oils obtained from the leaves of A. graveolens used for several applications, in particular a source of biological molecules with antioxidant and antimicrobial potential.


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