Do Hıgh Doses Of Proteın Supplements Affect Serum Lıpıd Profıles, Bıochemıcal And Glucose Metabolısm Markers?

Main Article Content

levent Cerit
Oguzhan Edebal
Hatice Kemal
Tugce Kıvılcım Bengisu Eken
Hamza Duygu


Protein supplement; serum creatinine; serum sodium; glucose metabolism; lipid profile


Aim: The aim of this study was to investigate the effects of protein supplements (PS) on blood biochemical, haematological, inflammatory parameters and lipid profile. Methods: Sixty-nine healthy subjects going to the gym for at least three months were included in the study. The subjects were divided into two groups according to their PS usage status. Fasting blood samples were taken from all of the subjects. Results: Among 69 subjects, 30(43%) were using PS, so were included in group 1, and 39 (57%) never used PS, therefore they consisted of group 2. The blood biochemical, haematological, inflammatory, glucose metabolism markers and lipid profiles were not different between the two groups. But, the subjects who used > 55 g PS/day had significantly higher serum creatinine values than subjects who never used PS. Similarly, the subjects who used  ≥ 30 g PS/day had significantly higher serum sodium levels than subjects who never used PS. Moreover, the subjects using PS for more than 10 months had significantly higher serum sodium levels when compared with subjects who did not use PS. Conclusion: Blood lipid profiles were not different in subjects using PS and in subjects who never used PS and they were not related to PS dosage.


Download data is not yet available.
Abstract 57 | PDF Downloads 34


1. Arciero PJ, Baur D, Connelly S, et al. Timed-daily ingestion of whey protein and exercise training reduces visceral adipose tissue mass and improves insulin resistance: the PRISE study. J Appl Physiol (1985) 2014 Jul 1;117(1):1-10.
2. Kemmler W, Wittke A, Bebenek M, et al. High-Intensity Resistance Training Methods with and without Protein Supplementation to Fight Cardiometabolic Risk in Middle-Aged Males: A Randomized Controlled Trial. Biomed Res Int. 2016;2016:9705287. doi: 10.1155/2016/9705287. Epub 2016 Jan 18.
3. Nicastro H, Gualano B, de Moraes WM, et al. Effects of creatinine supplementation on muscle wasting and glucose homeostasis in rats treated with dexamethasone. Amino Acids. 2012 May;42(5):1695-701.
4. Salgueiro RB, Gerlinger-Romero F, Guimaraes-Ferreira L, et al. Exercise training reverses the negative effects of chronic L-arginine supplementation on insulin sensitivity. Life Sci. 2017 Dec 15; 191:17-23.
5. Ohtani M, Maruyama K, Sugita M, et al. Amino acid supplementation affects hematological and biochemical parameters in elite rugby players. Biosci Biotechnol Biochem 2001 Sep;65(9):1970-6.
6. Ormsbee MJ, Rawal SR, Baur DA, et al. The effects of a multi-ingredient dietary supplement on body composition, adipokines, blood lipids, and metabolic health in overweight and obese men and women: a randomized controlled trial. J Int Soc Sports Nutr. 2014 Jul 26;11:37. doi: 10.1186/1550-2783-11-37. eCollection 2014.
7. Sheikholeslami Vatani D, Ahmadi Kani Golzar F. Changes in antioxidant status and cardiovascular risk factors of overweight young men after six weeks supplementation of whey protein isolate and resistance training. Appetite. 2012 Dec;59(3):673-8.
8. Rafraf M, Karimi M, Jafari A. Effect of L-carnitine supplementation in comparison with moderate aerobic training on serum inflammatory parameters in healthy obese women. J Sports Med Phys Fitness. 2015 Nov;55(11):1363-70.
9. Deminice R, Rosa FT, Franco GS, et al. Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition. 2013 Sep;29(9):1127-32.
10. Pala R, Genc E, Tuzcu M, et al. L-carnitine supplementation increases expression of PPAR-¥ and glucose transporters in skeletal muscle of chronically and acutely exercised rats. Cell Mol Biol(Noisy-le-grand). 2018 Jan 31;64(1):1-6.
11. Costa Junior JM, Rosa MR, Protzek AO, et al. Leucine supplementation does not affect protein turnover and impairs the beneficial effects of endurance training on glucose homeostasis in healthy mice. Amino Acids.2015 Apr;47(4):745-55.
12. Nicastro H, Zanchi NE, da Luz CR, et al. Effects of leucine supplementation and resistance exercise on dexamethasone-induced muscle atrophy and insulin resistance in rats. Nutrition. 2012 Apr;28(4):465-71.
13. Alvares TS, Conte-Junior CA, Silva JT, et al. L-arginine does not improve biochemical and hormonal response in trained runners after 4 weeks of supplementation. Nutr Res. 2014 Jan;34(1):31-9.
14. Cogan KE, Egan B. Effects of acute ingestion of whey protein with or without prior aerobic exercise on postprandial glycemia in type 2 diabetics. Eur J Appl Physiol. 2018 Sep;118(9):1959-1968.
15. Natarajan Sulochana K, Lakshmi S, Punitham R, et al. Effect of oral supplementation of free amino acids in type 2 diabetic patients- a pilot clinical trial. Med Sci Monit. 2002 Mar;8(3):CR131-7.
16. He Q, Kong X, Wu G, et al. Metabolomic analysis of the growing pigs to dietary L-arginine supplementation. Amino Acids. 2009 May;37(1):199-208.
17. Chen YM, Lin CL, Wei L, et al. Sake protein supplementation affects exercise performance and biochemical profiles in power-exercise-trained mice. Nutrients. 2016 Feb 20;8(2):106.doi:10.3390/nu8020106.
18. Schwingshackl L, Hoffmann G. Comparison of high vs. normal/low protein diets on renal function in subjects without chronic kidney disease: a systematic review and meta-analysis. PLoS ONE 2014;9(5):e97656.
19. Abuelgassim AO, Al-showayman SI. The effect of pumpkin (Cucurbitapepo L) seeds and L-arginine supplementation on serum lipid concentrations in atherogenic rats. Afr J Tradit Complement Altern Med. 2011 Oct 2;9(1):131-7.
20. Nabuco HCG, Tomeleri CM, Sugihara Junior P, et al.Effects of pre- or post-exercise whey PSation on body fat and metabolic and inflammatory profile in pre-conditioned older women: A randomized, double-blind, placebo-controlled trial. Nutr Metab Cardiovasc Dis. 2019 Mar;29(3):290-300.
21. Hayward S, Wilborn CD, Taylor LW, et al. Effects of a high protein and omega-3-enriched diet with or without creatinine supplementation on markers of soreness and inflammation during 5 consecutive days of high volume resistance exercise in females. J Sports Sci Med. 2016 Dec 1;15(4):704-14.

Most read articles by the same author(s)