Characteristics of disinfectants activity against multidrug-resistant clinical isolates
Disinfectants activity against multidrug resistant clinical isolates
Keywords:
health-care associated infections, multidrug resistance to antibiotics, sensitivity to antiseptics and disinfectantsAbstract
Background and aim: health-care associated infections (HCAI) significantly impair the quality and efficiency of medical care. Medical equipment`s parts are contaminated with opportunistic hospital strains leading to their spreading. Therefore, effective disinfection of medical equipment is one of the ways to solve a problem of HCAI. Methods: the research includes results of antimicrobial activity of biocides of different chemical structure (aqueous solutions of quaternary ammonium compounds, biguanides, oxidizers), which are used in medical practice as disinfectants for hospital environment, devices and equipment. Multidrug resistant clinical isolates with genetic markers of resistance were employed for comparative evaluation of ready-in-use germicides. Next microorganisms were used as test ones: clinical strains of methicillin-resistant S. aureus carrying mec A genes, carbapenemase-producing K. pneumoniae carrying blaOXA-48 gene, A. baumannii with blaOXA-72 gene, metallobetalactamase-producing P. aeruginosa carrying NDM-1 gene, strains of enterobacteria and non-fermenting gram-negative bacteria with a wide range of genes responsible for the production of aminotransferases that destroy aminoglycoside molecules. A quantitative suspension test was used to assess the activity of the tested biocides on some species of resistant microorganisms. Experimental study also includes antimicrobial efficacy testing of the disinfectant solutions, which was carried out on artificially contaminated with multidrug resistant clinical isolates fragments of polymer tubes of the respiratory circuit. Results: gram-negative bacteria showed a variable level of resistance to the studied germicides action, depending on the type of disinfectant solution and test microorganisms. Conclusions: the obtained results demonstrated that antibiotic-resistant strains of Gram-positive non-spore-forming bacteria were high susceptible to studied disinfectant solutions.
References
World Health Organization. The evolving threat of antimicrobial resistance: options for action. World Health Organization 2012 https://apps.who.int/iris/handle/10665/44812.
Sexton J, Tanner B, Maxwell S, et al. Reduction in the microbial load on high-touch surfaces in hospital rooms by treatment with a portable saturated steam vapor disinfection system. Am J Infect Control 2011 Oct; 39(8): 655-662. doi:10.1016/j.ajic.2010.11.009.
Agodi A, Barchitta M, Quattrocchi A, et al. Preventable proportion of intubation-associated pneumonia: Role of adherence to a care bundle. PLoS One. 2017 Sep 6;12(9):e0181170. doi: 10.1371/journal.pone.0181170.
Greenwood B. A global action plan for the prevention and control of pneumonia. Bull World Health Organ. 2008 May;86(5):322-2A. doi: 10.2471/blt.08.053348.
Zaragoza R., Vidal-Cortés P., Aguilar G. et al. Update of the treatment of nosocomial pneumonia in the ICU. Crit Care 24, 383 (2020). doi: 10.1186/s13054-020-03091-2.
Blot S, Koulenti D, Dimopoulos G, et al. Prevalence, risk factors, and mortality for ventilator-associated pneumonia in middle-aged, old, and very old critically ill patients. Crit Care Med. 2014; 42, 601–609. doi: 10.1097/01.ccm.0000435665.07446.50.
Nazarchuk O, Dmytriiev D, Dmytriiev K, Nazarchuk H, Zaletskiy B. Characteristics of infectious complications in critically ill patients. Wiad Lek 2018; 71(9): 1784-1792. PMID: 30737942.
Lansbury L, Lim B, Baskaran V, Lim Wei S. Co-infections in people with COVID-19: a systematic review and meta-analysis. J Infect. 2020 Aug; 81(2), 266-275. doi: 10.1016/j.jinf.2020.05.046.
Garcia-Vidal C, Sanjuan G, Moreno-García E, et al. Incidence of co-infections and superinfections in hospitalized patients with COVID-19: a retrospective cohort study. Clin Microbiol Infect. 2021;27(1):83-88. doi:10.1016/j.cmi.2020.07.041.
Levchenko B, Nazarchuk O, Dmytriiev D, Bahniuk N, Melnychenko M, Dmytriiev K. Adjunctive inhaled amikacin in infants with Ventilator-Associated Pneumonia optimizes the complex antimicrobial therapy: a pilot study. Acta Biomed 2023 Apr; 94(2): e2023084. doi: 10.23750/abm.v94i2.13910.
De Macedo V, Santos GS, Silva RN, et al. Healthcare-associated infections: a threat to the survival of patients with COVID-19 in intensive care units. J Hosp Infect. 2022;126:109-115. doi:10.1016/j.jhin.2022.05.013.
Boucher HW, Talbot GH, Bradley JS, et al. Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clin Infect Dis. 2009;48(1):1-12. doi:10.1086/595011.
Natham Н, Kondagadapu S, Kadiyala V, Mohan A, Chaudhury A, Samantaray A. Bacterial Colonisation and Antibiotic Sensitivity Profile of Endotracheal Tubes in Mechanically Ventilated Patients. J Clin Diagn Res. 2019;13(1): UC01-UC06. doi:10.7860/JCDR/2019/37402.12457.
Boisvert A, Cheng M.P, Sheppard D.C, Nguyen D. Microbial Biofilms in Pulmonary and Critical Care Diseases. Ann Am Thorac Soc. 2016; 13(9), 1615-1623. doi: 10.1513/AnnalsATS.201603-194FR.
Kovalchuk VP, Nazarchuk OA, Burkot VM, Fomina NS, Prokopchuk ZM, Dobrovanov O. Biofilm forming activity of non-fermenting gram-negative bacteria. Wiad Lek. 2021;74(2):252-256. PMID: 33813481.
Synetar EO. Biofilm formation by microorganisms and their importance in medicine. Bull of Probl of Biol and Med. 2018; 2(144), 59-63. doi:10.29254/2077-4214-2018-2-144-59-63.
Danin P, Girou Е, Legrand P, Louis B, Fodil R, Christov C. Description and microbiology of endotracheal tube biofilm in mechanically ventilated subjects. Respiratory Care 2015; 60(1), 21-29. doi:10.4187/respcare.02722.
Kampf G, Todt D, Pfaender S, Steinmann E. Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents. J Hosp Infect 2020; 104 (3): 246-251. doi:10.1016/j.jhin.2020.01.022.
Nahaichuk VI, Nazarchuk OA, Osadchuk NI, Dmytriiev DV, Nazarchuk HH. The analytical prognosis of the susceptibility to aminoglycosides and doxycycline in acinetobacter baumannii isolated from burns of intensive care unit patients. Wiad Lek 2018;71(3):705-709. PMID: 29783252.
Palii GK, Pavlyuk SV, Dudar AO, Palii DV, Nazarchuk OA, Agafonov KV. Justification of the use of antiseptic drugs in the system of preventive and curative measures (literature review). Bukovyna Medical Bulletin 2018; 422 (22): 138-146.
Zamudio R, Oggioni MR, Gould IM, Hijazi K. Time for biocide stewardship? Nature Microbiology 2019 May; 4(5): 732–733. doi:10.1038/s41564-019-0360-6.
Derkach NM, Shtrigol’ SY, Laryanovska YB, Koshova OY, Kovaleva EO. Specific toxicity of the drug "Dekasan". Clin and Exp Path. 2016; 15:2(56): 1: 59-66. doi:10.24061/1727-4338.XV.2.56.2016.14
Li Z, Liu H, Ma L, Shang S, Song Z. Surface modification of silicone elastomer with rosin acid-based quaternary ammonium salt for antimicrobial and biocompatible properties. Mater and Des. 2020: 189(2):108493. doi:10.1016/j.matdes.2020.108493.
Mathew RT, Cooney RP, Doyle CC, Swift S, Haessler C. Anchored quaternary ammonium salts adsorbed on polyurethane film surfaces. Progr in Org Coat. 2020; 138: 105343. doi:10.1016/j.porgcoat.2019.105343
Salmanov AG, Marievsky VF, Khobzei MK. Bacterial resistance to antiseptics and disinfectants. Ukrainian Medical Journal 2010: 6 (80): XI-XII.
Wand ME, Bock LJ, Bonney LC, Sutton JM. Mechanisms of increased resistance to chlorhexidine and cross-resistance to colistin following exposure of klebsiella pneumonia clinical isolates to chlorhexidine. Antimicrob Agents Chemother 2017; 61(1): e01162–16. doi: 10.1128/AAC.01162-16.
Buxser S. Has resistance to chlorhexidine increased among clinically-relevant bacteria? A systematic review of time course and subpopulation data. PLoS ONE 2021 Aug; 16 (8): e0256336. doi.org/10.1371/journal.pone.0256336.
M100: Antimicrobial Susceptibility Testing Standards [Internet]. Clinical & Laboratory Standards Institute. CLSI; 2019. Available from: https://clsi.org/standards/products/microbiology/documents/m100/
National Center for Biotechnology Information (NCBI) [Internet]. Available from: https://www.ncbi.nlm.nih.gov/genbank/
Basic Local Alignment Search Tool [Internet]. Available from: https://blast.ncbi.nlm.nih.gov/Blast.cgi
EN 13727:2012+A2:2015 Chemical disinfectants and antiseptics —Quantitative suspension test for the evaluation of bactericidal activity in the medical area —Test method and requirements (phase 2, step 1) [Internet] Available from: https://www.en-standard.eu
Fraise AP. Choosing disinfectants. J Hosp Infect 1999 Dec; 43(1): 255–64. doi:10.1016/s0195-6701(99)90421-8.
Dmytriiev D, Melnychenko M, Dobrovanov O, Nazarchuk O, Vidiscak M. Perioperative hemodynamic protective assessment of adaptive support ventilation usage in pediatric surgical patients. Acute and Crit Care 2022;37(4):636-643. doi:10.4266/acc.2022.00297.
Аbreu АС, Tavares RR, Borges A, Mergulhão F, Simões M. Current and emergent strategies for disinfection of hospital environments. J. Antimicrob. Chemother. 2013; 68(12): 2718–2732. doi:10.1093/jac/dkt281.
Kovalchuk V.P., Kondratiuk V.M., Kovalenko I.M., Burkot V.M. Phenotypic and genotypic determinants of antibiotic resistance of gram-negative bacteria-etiological factors of infectious complications of war wounds. Mikrobiolohichnyi Zhurnal 2019; 81(1): 61-71. doi:10.15407/microbiolj81.01.061.
Kondratiuk V, Jones BT, Kovalchuk V, et al. Phenotypic and genotypic characterization of antibiotic resistance in military hospital-associated bacteria from war injuries in the Eastern Ukraine conflict between 2014 and 2020. J Hosp Infect. 2021;112:69-76. doi:10.1016/j.jhin.2021.03.020.
Rutala WA, Weber DJ. Guideline for Disinfection and Sterilization in Healthcare Facilities, Healthcare Infection Control Practices Advisory Committee (HICPAC). Atlanta, GA, USA: CDC. 2008. https://www.cdc.gov/infectioncontrol/guidelines/disinfection/.
Falagas ME, Thomaidis PC, Kotsantis IK, Sgouros K, Samonis G, Karageorgopoulos DE. Airborne hydrogen peroxide for disinfection of the hospital environment and infection control: a systematic review. J Hosp Infect. 2011 Jul;78(3):171-7. doi: 10.1016/j.jhin.2010.12.006.
Vijayakumar R, Saleh Al-Aboody M, AlFonaisan MK, Alturaiki W, Mickymaray S, Premanathan M, et al. Determination of minimum inhibitory concentrations of common biocides to multidrug-resistant gram-negative bacteria. Applied Medical Research 2016; 2(3): 56-62, doi: 10.5455/amr.20161012082036.
Тheraud M, Bedouin Y, Guiguen C, Gangneux J-P. Efficacy of antiseptics and disinfectants on clinical and environmental yeast isolates in planktonic and biofilm conditions. J Med Microbiol 2004; 53(Pt10): 1013 –1018. doi: 10.1099/jmm.0.05474-0.
Humenyuk MI, Humenyuk GL, Opimakh SG. Effectiveness of decamethoxine against complex viruses, regardless of their antigenic structure: prospects for use in modern viral diseases of the respiratory tract. Current Infectology 2020; 8(1): 25-33. doi.org/10.22141/2312-413x.8.1.2020.196168.
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