A pilot study on the preventative potential of alpha-cyclodextrin and hydroxytyrosol against SARS-CoV-2 transmission

A pilot study on the preventative potential of alpha-cyclodextrin and hydroxytyrosol against SARS-CoV-2 transmission

Authors

  • Mahmut Cerkez Ergoren Department of Medical Biology, Faculty of Medicine, Near East University, Nicosia, Cyprus; DESAM Institute, Near East University, Nicosia, Cyprus
  • Stefano Paolacci MAGI'S LAB
  • Elena Manara MAGI EUREGIO, Bolzano, Italy
  • Astrit Dautaj EBTNA-LAB, Rovereto (TN), Italy
  • Kristjana Dhuli EBTNA-LAB, Rovereto (TN), Italy
  • Kyrylo Anpilogov EBTNA-LAB, Rovereto (TN), Italy
  • Giorgio Camilleri EBTNA-LAB, Rovereto (TN), Italy
  • Huseyin Kaya Suer Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Near East University, Nicosia, Cyprus
  • Murat Sayan DESAM Institute, Near East University, Nicosia, Cyprus; PCR Unit, Kocaeli University Hospital, Kocaeli, Turkey
  • Gulten Tuncel Department of Medical Biology, Faculty of Medicine, Near East University, Nicosia, Cyprus; DESAM Institute, Near East University, Nicosia, Cyprus
  • Nazife Sultanoglu DESAM Institute, Near East University, Nicosia, Cyprus
  • Marco Farronato Department of Biomedical, Surgical and Dental Sciences, School of Dentistry, University of Milan; Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, Milan, Italy
  • Gianluca Martino Tartaglia Department of Biomedical, Surgical and Dental Sciences, School of Dentistry, University of Milan; Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, Milan, Italy
  • Munis Dundar Department of Medical Genetics, Erciyes University Faculty of Medicine, Kayseri, Turkey
  • Giampietro Farronato Department of Biomedical, Surgical and Dental Sciences, School of Dentistry, University of Milan; Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, Milan, Italy
  • Irfan Suat Gunsel Near East University, Nicosia, Cyprus
  • Matteo Bertelli MAGI’S LAB, Rovereto (TN), Italy; MAGI EUREGIO, Bolzano, Italy; EBTNA-LAB, Rovereto (TN), Italy
  • Tamer Sanlidag DESAM Institute, Near East University, Nicosia, Cyprus

Keywords:

SARS-CoV-2, ACE2, hydroxytyrosol, α-cyclodextrin

Abstract

Background and aim of the work: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the current pandemics. This virus attacks the cells by binding to the transmembrane angiotensin I converting enzyme 2. In this study, we experimented a food supplement containing alpha-cyclodextrin and hydroxytyrosol for the improvement of the defenses against the SARS-CoV-2. Hydroxytyrosol has anti-viral properties and is able to reduce the serum lipids in mice. α-cyclodextrin has the ability to deplete sphingolipids and phospholipids from the cellular membranes. The aim of the present preliminary open non-controlled interventional study was to evaluate the efficacy of alpha-cyclodextrin and hydroxytyrosol in improving defenses against SARS-CoV-2. Methods: Fifty healthy volunteers at a higher risk of SARS-CoV-2 infection from Northern Cyprus and six positive individuals for SARS-CoV-2 were enrolled in this study. The in silico prediction was performed using D3DOCKING to evaluate the interactions of hydroxytyrosol and alpha-cyclodextrin with proteins involved in the SARS-CoV-2 endocytosis. Results: The 50 volunteers did not become positive in 15 days for SARS-CoV-2 after the administration of the compound for two weeks, despite they were at higher risk of infection than the general population. Interestingly, in the cohort of six positive patients, two patients were administered the spray and became negative after five days, despite the viral load was higher in the treated subjects than the untreated patients who became negative after ten days. In addition, we identified possible interactions among hydroxytyrosol and alpha-cyclodextrin with the protein Spike and the human proteins ACE2 and TMPRSS2. Conclusions: We reported on the results of the possible role of alpha-cyclodextrin and hydroxytyrosol in improving defenses against SARS-CoV-2. The next step will be the administration of the compound to a larger cohort in a controlled study to confirm the reduction of the infection rate of SARS-CoV-2 in the treated subjects.

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Published

09-11-2020

How to Cite

1.
A pilot study on the preventative potential of alpha-cyclodextrin and hydroxytyrosol against SARS-CoV-2 transmission. Acta Biomed [Internet]. 2020 Nov. 9 [cited 2024 May 25];91(13-S):e2020022. Available from: https://mattioli1885journals.com/index.php/actabiomedica/article/view/10817

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