Interleukin 9 serum level and single nucleotide polymorphism in patients with asthma

Main Article Content

Seyed Alireza Mahdaviani
Mahsa Eskian
Mirhojjat Khorasanizadeh
Bahram Bashardoost
Sabereh Tashayoie Nejad
Hamid Reza Jamaati
Arezou Rezaei
Maryam Sadr
Zahra Aryan
Nima Rezaei

Keywords

ASTHMA, INTERLEUKIN-9, POLYMORPHISM

Abstract

Background:  Asthma is a chronic inflammatory disease of airways which accounts for a huge economic, morbidity and mortality burden. There are different cytokines that contribute to asthma pathophysiology. Learning about these cytokines leads to attaining novel anti-inflammatory treatments for asthma control.


Objectives: The objective of this study is to investigate the association between interleukin-9 serum level and gene polymorphism with asthma susceptibility.


Methods: This was a case-control study of 70 asthmatic patients and 77 healthy control adults aged 18-60. Asthma diagnosis and severity were based on physician diagnosis, pulmonary function test (PFT) and 2016 guild line of Global Initiative for Asthma (GINA). Interleukin 9(IL -9) serum level was measured using sandwich enzyme linked immunosorbent assay.  IL9 promoter single nucleotide polymorphism (SNP) (rs2069882) was also assessed using Real-Time PCR System.


Results: There was no significant association between IL-9 SNP polymorphism and asthma. IL-9 serum level was significantly associated with asthma susceptibility (p value= 0.016) and absolute eosinophil count (AEC) (P value=0.033) however its corelation with atopic asthma type, asthma sivierity and Immunoglubin E serum level were not statistically significant.


Conclusion: Although there was no association between IL-9 SNP and asthma, but IL-9 serum level was significantly correlated with asthma susceptibility and AEC.

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