Serum lipidomics in diagnostics of sarcoidosis

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Beata Toczylowska
Dariusz Jastrzebski
Sabina Kostorz
Elzbieta Zieminska
Dariusz Ziora

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Abstract

The aim of this study was to determine the use of the lipid profile of patients with sarcoidosis and compare it with healthy subjects. We assume that lipid profile of serum in sarcoidosis differs from the lipid profile of control subjects. Serum was collected from 14 patients with II stage of sarcoidosis and 14 control subjects (healthy volunteers). Proton NMR spectroscopy combined with discriminant analyses, OPLS-DA (orthogonal partial least squares projections to latent structures discriminant analysis), was used. Thirty four NMR signals of lipid compounds were selected. OPLS-DA model consisted of three components and very good explain the data and also predict the data. Discriminant analysis correctly classified patients according to their groups for 92.9% of sarcoidose and 100% of control. From multivariate discriminant analysis we obtain a list of potential biomarkers which are statistically significant and which separate one class from another. These biomarkers are statistically significant, but not necessarily biochemically significant. They may have biochemical significance and they may be the biomarkers we are interested in, however, this must be established through extensive testing. Presented method allows distinguishing between healthy subject and sarcoidosis patients.

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