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Lactobacillus Plantarum, Linoelic acid metabolites, GC MS
Summary. Objective: Lactobacillus Plantarum has the capability to convert polyunsaturated fatty acids, e.g. linoleic acid (LA) into bioactive and less toxic free saturated fatty acids. In this study we focused to evaluate the ability of six probiotic strains of L. Plantarum strains to produce linoleic acid (LA) metabolites in the medium supplemented with differential concentrations of LA. Method: L. Plantarum strains were grown in MRS medium containing LA from 1% to 10%, and the LA metabolites formed in the medium were identified and quantitated by GC-MS. Result: In respect to the identified LA metabolites, a total of 4 metabolites were detected including linoelaidic acid, 9,12-octadecadienoic acid (Z, Z), 9,12-Octadecadienoic acid, methyl ester, and trans,trans-9,12-octadecadienoic acid, propyl ester. In which, the later one was produced by the all six strains of L. Plantarum, and the linoelaidic acid isomer was produced by four of the tested strains (i.e L. Plantarum 1-2, 2-3, 12-4, and 12-5). Whereas, 9,12-Octadecadienoic acid (Z, Z) was only produced by the L. Plantarum 4-5, and 9,12-Octadecadienoic acid, methyl ester was also produced only by L. Plantarum 12-4. Among all the six L. Plantarum strains the best reported strain was L. Plantarum 2-3 which showed maximum growth and conversion of Linoleic Acid to different metabolites from 1% to 10 % of LA supplied while L. Plantarum 12-5 showed maximum growth and conversion at higher concentration of LA supplied and showed minimal growth and conversion at lower concentration of MRS LA. Conclusion: Among all the six L. Plantarum strains, the best reported strain was L. Plantarum 2-3 which showed maximum growth and conversion of Linoleic Acid to different metabolites.
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