Antinociceptive activity investigation of the methanolic crude extract of Costus speciosus in Mice

Shofiul Azam, Prawej Ansari, Shahnaz Jalil, Abdunasir Hussain Ibrahim, Nasrin Sultana, Mohammad Mosharraf Hossain, Jaysee Muhammad Naveed, Mohammed Forhad Hossain


Goal: This study was designed to investigate the anti-inflammatory and analgesic activity of one of common Bangladeshi medicinal plant, Costus speciosus. Methods & materials: We have included the phytochemical investigation before proceeding to our major objective. The seeds of Costus speciosus (MECS) were extracted using methanol. MECS have been used in in vitro and in vivo investigation for possible analgesic and anti-inflammatory effect. In this pre-designed study Formalin-induced writhing test and Tail immersion test was included. Results: The methanol extract of C. speciosus (at 500 mg/kg) showed significant (P<0.01) anti-inflammatory activity (51.09±1.67% and 75.93±2.58% shown by standard) on red blood cell membrane stabilization study, and the crude extract have included 75.15±2.33% (p<0.05) of protection against protein denaturation. In tail immersion study the extract at the dose of 500 mg/kg increases the response time up to 5.97±0.11 second, which was close enough to reference group (8.51±0.21 second) at 90 min of drug administration. C. speciosus relapse the writhing, up to 53.97% during first phase of inflammation induced by formalin, it also reduced pain substantially (59.89%; p<0.05) at second phase of study, where standard group showed 73.78% pain reduction. Conclusion: This study presents a very good potentiality in inflammatory mediators inhibition. From the result of our study, it is clear that it possess analgesic and anti-inflammatory activity. Further study is suggested for active compound isolation and adjustment.


analgesic, anti-inflammatory, C. speciosus, HRBC, inflammation, protein denaturation

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ISSN: 1129-8723